Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)

Autores
Cáceres, Mariano; Drago, A.; Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The infestations of Cimex lectularius L. (Heteroptera: Cimicidae) registered in the last decades have been influenced by several human activities, including international tourism and commerce. Moreover, the development of insecticide resistance and careless pest control strategies contributed to the dispersal of bed bugs. Given the complexity of the topic, distinguishing physiological and molecular mechanisms involved in resistance can help design proper control tools and limit the resistance spread. Here we determined the susceptibility to deltamethrin and imidacloprid in bed bugs collected in Italy. Also, we assessed the role of esterases and P450 monooxygenases by direct enzymatic activity measurement and inhibition by synergism bioassays. Our results showed that the field-collected colony exhibited high resistance ratios to imidacloprid and deltamethrin (757 and >60,000 times, respectively) compared to the susceptible colony. Moreover, resistant bed bugs showed increased activity of esterases and P450 monooxygenases. The synergistic effect of piperonyl butoxide (PBO) suggests the significant contribution of both enzymatic groups as detoxification pathways implicated in pyrethroid-resistant bed bugs. Further investigations are needed to unravel the biochemical and molecular basis involved in the resistant phenotype for developing novel strategies for pest control.
Fil: Cáceres, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Drago, A.. No especifíca;
Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Materia
INSECTICIDE RESISTANCE
ENZYMATIC ACTIVITY
DELTAMETHRIN
IMIDACLOPRID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/233279
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/233279
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)Cáceres, MarianoDrago, A.Santo Orihuela, Pablo LuisVassena, Claudia VivianaINSECTICIDE RESISTANCEENZYMATIC ACTIVITYDELTAMETHRINIMIDACLOPRIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The infestations of Cimex lectularius L. (Heteroptera: Cimicidae) registered in the last decades have been influenced by several human activities, including international tourism and commerce. Moreover, the development of insecticide resistance and careless pest control strategies contributed to the dispersal of bed bugs. Given the complexity of the topic, distinguishing physiological and molecular mechanisms involved in resistance can help design proper control tools and limit the resistance spread. Here we determined the susceptibility to deltamethrin and imidacloprid in bed bugs collected in Italy. Also, we assessed the role of esterases and P450 monooxygenases by direct enzymatic activity measurement and inhibition by synergism bioassays. Our results showed that the field-collected colony exhibited high resistance ratios to imidacloprid and deltamethrin (757 and >60,000 times, respectively) compared to the susceptible colony. Moreover, resistant bed bugs showed increased activity of esterases and P450 monooxygenases. The synergistic effect of piperonyl butoxide (PBO) suggests the significant contribution of both enzymatic groups as detoxification pathways implicated in pyrethroid-resistant bed bugs. Further investigations are needed to unravel the biochemical and molecular basis involved in the resistant phenotype for developing novel strategies for pest control.Fil: Cáceres, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Drago, A.. No especifíca;Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaWageningen Academic Publishers2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/233279Cáceres, Mariano; Drago, A.; Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae); Wageningen Academic Publishers; Journal of the European Mosquito Control Association; 2-2023; 1-62054-930XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.52004/JEMCA2022.0003info:eu-repo/semantics/altIdentifier/doi/10.52004/JEMCA2022.0003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:57Zoai:ri.conicet.gov.ar:11336/233279instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:57.315CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
title Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
spellingShingle Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
Cáceres, Mariano
INSECTICIDE RESISTANCE
ENZYMATIC ACTIVITY
DELTAMETHRIN
IMIDACLOPRID
title_short Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
title_full Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
title_fullStr Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
title_full_unstemmed Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
title_sort Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)
dc.creator.none.fl_str_mv Cáceres, Mariano
Drago, A.
Santo Orihuela, Pablo Luis
Vassena, Claudia Viviana
author Cáceres, Mariano
author_facet Cáceres, Mariano
Drago, A.
Santo Orihuela, Pablo Luis
Vassena, Claudia Viviana
author_role author
author2 Drago, A.
Santo Orihuela, Pablo Luis
Vassena, Claudia Viviana
author2_role author
author
author
dc.subject.none.fl_str_mv INSECTICIDE RESISTANCE
ENZYMATIC ACTIVITY
DELTAMETHRIN
IMIDACLOPRID
topic INSECTICIDE RESISTANCE
ENZYMATIC ACTIVITY
DELTAMETHRIN
IMIDACLOPRID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The infestations of Cimex lectularius L. (Heteroptera: Cimicidae) registered in the last decades have been influenced by several human activities, including international tourism and commerce. Moreover, the development of insecticide resistance and careless pest control strategies contributed to the dispersal of bed bugs. Given the complexity of the topic, distinguishing physiological and molecular mechanisms involved in resistance can help design proper control tools and limit the resistance spread. Here we determined the susceptibility to deltamethrin and imidacloprid in bed bugs collected in Italy. Also, we assessed the role of esterases and P450 monooxygenases by direct enzymatic activity measurement and inhibition by synergism bioassays. Our results showed that the field-collected colony exhibited high resistance ratios to imidacloprid and deltamethrin (757 and >60,000 times, respectively) compared to the susceptible colony. Moreover, resistant bed bugs showed increased activity of esterases and P450 monooxygenases. The synergistic effect of piperonyl butoxide (PBO) suggests the significant contribution of both enzymatic groups as detoxification pathways implicated in pyrethroid-resistant bed bugs. Further investigations are needed to unravel the biochemical and molecular basis involved in the resistant phenotype for developing novel strategies for pest control.
Fil: Cáceres, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Drago, A.. No especifíca;
Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina
description The infestations of Cimex lectularius L. (Heteroptera: Cimicidae) registered in the last decades have been influenced by several human activities, including international tourism and commerce. Moreover, the development of insecticide resistance and careless pest control strategies contributed to the dispersal of bed bugs. Given the complexity of the topic, distinguishing physiological and molecular mechanisms involved in resistance can help design proper control tools and limit the resistance spread. Here we determined the susceptibility to deltamethrin and imidacloprid in bed bugs collected in Italy. Also, we assessed the role of esterases and P450 monooxygenases by direct enzymatic activity measurement and inhibition by synergism bioassays. Our results showed that the field-collected colony exhibited high resistance ratios to imidacloprid and deltamethrin (757 and >60,000 times, respectively) compared to the susceptible colony. Moreover, resistant bed bugs showed increased activity of esterases and P450 monooxygenases. The synergistic effect of piperonyl butoxide (PBO) suggests the significant contribution of both enzymatic groups as detoxification pathways implicated in pyrethroid-resistant bed bugs. Further investigations are needed to unravel the biochemical and molecular basis involved in the resistant phenotype for developing novel strategies for pest control.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/233279
Cáceres, Mariano; Drago, A.; Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae); Wageningen Academic Publishers; Journal of the European Mosquito Control Association; 2-2023; 1-6
2054-930X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/233279
identifier_str_mv Cáceres, Mariano; Drago, A.; Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae); Wageningen Academic Publishers; Journal of the European Mosquito Control Association; 2-2023; 1-6
2054-930X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.52004/JEMCA2022.0003
info:eu-repo/semantics/altIdentifier/doi/10.52004/JEMCA2022.0003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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