Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging

Autores
Arreche, Noelia Daniela; Cernadas, Gustavo; Puchulu, María Bernardita; Fellet, Andrea L.; Balaszczuk, Ana María
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Caveolin-1 is a negative regulator of endothelial nitric oxide synthase (eNOS), as its interaction and binding suppress the activityof eNOS by making caveolin–eNOS complex. The critical role of caveolin-1 and eNOS in the renal medulla during hypovolemic state and aging isunclear.Objective: To investigate changes in the renal medulla the activity and protein levels of the different NOS forms, changes in the abundance ofcaveolin-1 and caveolin-1/eNOS association in response to acute hemorrhage in young and middle-aged rats.Methods: In the present study we determined: i) NOS activity and protein levels of the different NOS isoforms, ii) the abundance of caveolin-1and, iii) caveolin-1/eNOS association during hypovolemic state of young and middle-aged rats in the kidney.Results: We have demonstrated that caveolin-1 is involved in the regulation of renal NOS activity in response to acute hemorrhage and aging.Conclusion: Our data showed that association/dissociation of caveolin-1 and eNOS in renal medulla play a critical role in preserving the renalfunction during hypovolemic state in young and middle-aged rats.
Fil: Arreche, Noelia Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cernadas, Gustavo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Puchulu, María Bernardita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Balaszczuk, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia
CAVEOLIN 1
NITRIC OXIDE
RENAL MEDULLA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212576
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212576
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and AgingArreche, Noelia DanielaCernadas, GustavoPuchulu, María BernarditaFellet, Andrea L.Balaszczuk, Ana MaríaCAVEOLIN 1NITRIC OXIDERENAL MEDULLAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Caveolin-1 is a negative regulator of endothelial nitric oxide synthase (eNOS), as its interaction and binding suppress the activityof eNOS by making caveolin–eNOS complex. The critical role of caveolin-1 and eNOS in the renal medulla during hypovolemic state and aging isunclear.Objective: To investigate changes in the renal medulla the activity and protein levels of the different NOS forms, changes in the abundance ofcaveolin-1 and caveolin-1/eNOS association in response to acute hemorrhage in young and middle-aged rats.Methods: In the present study we determined: i) NOS activity and protein levels of the different NOS isoforms, ii) the abundance of caveolin-1and, iii) caveolin-1/eNOS association during hypovolemic state of young and middle-aged rats in the kidney.Results: We have demonstrated that caveolin-1 is involved in the regulation of renal NOS activity in response to acute hemorrhage and aging.Conclusion: Our data showed that association/dissociation of caveolin-1 and eNOS in renal medulla play a critical role in preserving the renalfunction during hypovolemic state in young and middle-aged rats.Fil: Arreche, Noelia Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Cernadas, Gustavo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Puchulu, María Bernardita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Balaszczuk, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaBiomedGrid2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212576Arreche, Noelia Daniela; Cernadas, Gustavo ; Puchulu, María Bernardita; Fellet, Andrea L.; Balaszczuk, Ana María ; Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging; BiomedGrid; American Journal of Biomedical Science & Research; 11; 4; 1-2021; 332-3382642-1747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biomedgrid.com/fulltext/volume11/association-dissociation-of-caveolin-1-and-nitric-oxide-synthase-in-preserving-renal-medulla-function-during-hemorrhage-and-aging.001655.phpinfo:eu-repo/semantics/altIdentifier/doi/10.34297/AJBSR.2021.11.001655info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:36Zoai:ri.conicet.gov.ar:11336/212576instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:36.472CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
title Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
spellingShingle Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
Arreche, Noelia Daniela
CAVEOLIN 1
NITRIC OXIDE
RENAL MEDULLA
title_short Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
title_full Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
title_fullStr Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
title_full_unstemmed Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
title_sort Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging
dc.creator.none.fl_str_mv Arreche, Noelia Daniela
Cernadas, Gustavo
Puchulu, María Bernardita
Fellet, Andrea L.
Balaszczuk, Ana María
author Arreche, Noelia Daniela
author_facet Arreche, Noelia Daniela
Cernadas, Gustavo
Puchulu, María Bernardita
Fellet, Andrea L.
Balaszczuk, Ana María
author_role author
author2 Cernadas, Gustavo
Puchulu, María Bernardita
Fellet, Andrea L.
Balaszczuk, Ana María
author2_role author
author
author
author
dc.subject.none.fl_str_mv CAVEOLIN 1
NITRIC OXIDE
RENAL MEDULLA
topic CAVEOLIN 1
NITRIC OXIDE
RENAL MEDULLA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Caveolin-1 is a negative regulator of endothelial nitric oxide synthase (eNOS), as its interaction and binding suppress the activityof eNOS by making caveolin–eNOS complex. The critical role of caveolin-1 and eNOS in the renal medulla during hypovolemic state and aging isunclear.Objective: To investigate changes in the renal medulla the activity and protein levels of the different NOS forms, changes in the abundance ofcaveolin-1 and caveolin-1/eNOS association in response to acute hemorrhage in young and middle-aged rats.Methods: In the present study we determined: i) NOS activity and protein levels of the different NOS isoforms, ii) the abundance of caveolin-1and, iii) caveolin-1/eNOS association during hypovolemic state of young and middle-aged rats in the kidney.Results: We have demonstrated that caveolin-1 is involved in the regulation of renal NOS activity in response to acute hemorrhage and aging.Conclusion: Our data showed that association/dissociation of caveolin-1 and eNOS in renal medulla play a critical role in preserving the renalfunction during hypovolemic state in young and middle-aged rats.
Fil: Arreche, Noelia Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cernadas, Gustavo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Puchulu, María Bernardita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fellet, Andrea L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Balaszczuk, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description Background: Caveolin-1 is a negative regulator of endothelial nitric oxide synthase (eNOS), as its interaction and binding suppress the activityof eNOS by making caveolin–eNOS complex. The critical role of caveolin-1 and eNOS in the renal medulla during hypovolemic state and aging isunclear.Objective: To investigate changes in the renal medulla the activity and protein levels of the different NOS forms, changes in the abundance ofcaveolin-1 and caveolin-1/eNOS association in response to acute hemorrhage in young and middle-aged rats.Methods: In the present study we determined: i) NOS activity and protein levels of the different NOS isoforms, ii) the abundance of caveolin-1and, iii) caveolin-1/eNOS association during hypovolemic state of young and middle-aged rats in the kidney.Results: We have demonstrated that caveolin-1 is involved in the regulation of renal NOS activity in response to acute hemorrhage and aging.Conclusion: Our data showed that association/dissociation of caveolin-1 and eNOS in renal medulla play a critical role in preserving the renalfunction during hypovolemic state in young and middle-aged rats.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212576
Arreche, Noelia Daniela; Cernadas, Gustavo ; Puchulu, María Bernardita; Fellet, Andrea L.; Balaszczuk, Ana María ; Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging; BiomedGrid; American Journal of Biomedical Science & Research; 11; 4; 1-2021; 332-338
2642-1747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212576
identifier_str_mv Arreche, Noelia Daniela; Cernadas, Gustavo ; Puchulu, María Bernardita; Fellet, Andrea L.; Balaszczuk, Ana María ; Association/Dissociation of Caveolin-1 and Nitric Oxide Synthase in Preserving Renal Medulla Function during Hemorrhage and Aging; BiomedGrid; American Journal of Biomedical Science & Research; 11; 4; 1-2021; 332-338
2642-1747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biomedgrid.com/fulltext/volume11/association-dissociation-of-caveolin-1-and-nitric-oxide-synthase-in-preserving-renal-medulla-function-during-hemorrhage-and-aging.001655.php
info:eu-repo/semantics/altIdentifier/doi/10.34297/AJBSR.2021.11.001655
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BiomedGrid
publisher.none.fl_str_mv BiomedGrid
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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