Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection

Autores
Aparicio, Jose Luis; Ottobre Saborido, Macarena Aylén; Duhalde Vega, Maite; Coutelier, Jean-Paul; Van Snick, Jacques; Retegui, Lilia Alicia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouse MHV-A59-infection. MAb anti IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59-infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not affect Ig levels.Serum IFNγ was detected in mice infected with MHV-A59, and its concentration increased after MAb anti-IL-17A administration. Besides, MAb anti-IFNγ greatly augmented transaminase plasmatic levels. IL-17A neutralization did not affect MHV-A59-induction of HMGB1 liberation and slightly augmented plasmatic uric acid concentration. However, mice treated with the MAb failed to produce autoAb to FAH. The above results suggest a reciprocal regulation of Th1 and Th17 cells acting on the different MHV-A59 effects. In addition, it is proposed that IL-17A is involved in alarmins adjuvant effects leading to autoAb expression.
Fil: Aparicio, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ottobre Saborido, Macarena Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Duhalde Vega, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Coutelier, Jean-Paul. Université Catholique de Louvain; Bélgica
Fil: Van Snick, Jacques. Ludwig Institute for Cancer Research; Bélgica
Fil: Retegui, Lilia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
IL-17
IFNg
MHV
autoAb
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47349
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47349
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infectionAparicio, Jose LuisOttobre Saborido, Macarena AylénDuhalde Vega, MaiteCoutelier, Jean-PaulVan Snick, JacquesRetegui, Lilia AliciaIL-17IFNgMHVautoAbhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouse MHV-A59-infection. MAb anti IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59-infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not affect Ig levels.Serum IFNγ was detected in mice infected with MHV-A59, and its concentration increased after MAb anti-IL-17A administration. Besides, MAb anti-IFNγ greatly augmented transaminase plasmatic levels. IL-17A neutralization did not affect MHV-A59-induction of HMGB1 liberation and slightly augmented plasmatic uric acid concentration. However, mice treated with the MAb failed to produce autoAb to FAH. The above results suggest a reciprocal regulation of Th1 and Th17 cells acting on the different MHV-A59 effects. In addition, it is proposed that IL-17A is involved in alarmins adjuvant effects leading to autoAb expression.Fil: Aparicio, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Ottobre Saborido, Macarena Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Duhalde Vega, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Coutelier, Jean-Paul. Université Catholique de Louvain; BélgicaFil: Van Snick, Jacques. Ludwig Institute for Cancer Research; BélgicaFil: Retegui, Lilia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaJohn Libbey Eurotext Ltd2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47349Aparicio, Jose Luis; Ottobre Saborido, Macarena Aylén; Duhalde Vega, Maite; Coutelier, Jean-Paul; Van Snick, Jacques ; et al.; Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection; John Libbey Eurotext Ltd; European Cytokine Network; 28; 3; 9-2017; 111-1171148-5493CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jle.com/fr/revues/ecn/e-docs/effects_of_interleukin_17a_il_17a_neutralization_on_murine_hepatitis_virus_mhv_a59_infection_310871/article.phtmlinfo:eu-repo/semantics/altIdentifier/doi/10.1684/ecn.2017.0399info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:32Zoai:ri.conicet.gov.ar:11336/47349instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:32.338CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
title Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
spellingShingle Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
Aparicio, Jose Luis
IL-17
IFNg
MHV
autoAb
title_short Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
title_full Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
title_fullStr Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
title_full_unstemmed Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
title_sort Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection
dc.creator.none.fl_str_mv Aparicio, Jose Luis
Ottobre Saborido, Macarena Aylén
Duhalde Vega, Maite
Coutelier, Jean-Paul
Van Snick, Jacques
Retegui, Lilia Alicia
author Aparicio, Jose Luis
author_facet Aparicio, Jose Luis
Ottobre Saborido, Macarena Aylén
Duhalde Vega, Maite
Coutelier, Jean-Paul
Van Snick, Jacques
Retegui, Lilia Alicia
author_role author
author2 Ottobre Saborido, Macarena Aylén
Duhalde Vega, Maite
Coutelier, Jean-Paul
Van Snick, Jacques
Retegui, Lilia Alicia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv IL-17
IFNg
MHV
autoAb
topic IL-17
IFNg
MHV
autoAb
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouse MHV-A59-infection. MAb anti IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59-infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not affect Ig levels.Serum IFNγ was detected in mice infected with MHV-A59, and its concentration increased after MAb anti-IL-17A administration. Besides, MAb anti-IFNγ greatly augmented transaminase plasmatic levels. IL-17A neutralization did not affect MHV-A59-induction of HMGB1 liberation and slightly augmented plasmatic uric acid concentration. However, mice treated with the MAb failed to produce autoAb to FAH. The above results suggest a reciprocal regulation of Th1 and Th17 cells acting on the different MHV-A59 effects. In addition, it is proposed that IL-17A is involved in alarmins adjuvant effects leading to autoAb expression.
Fil: Aparicio, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ottobre Saborido, Macarena Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Duhalde Vega, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Coutelier, Jean-Paul. Université Catholique de Louvain; Bélgica
Fil: Van Snick, Jacques. Ludwig Institute for Cancer Research; Bélgica
Fil: Retegui, Lilia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouse MHV-A59-infection. MAb anti IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59-infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not affect Ig levels.Serum IFNγ was detected in mice infected with MHV-A59, and its concentration increased after MAb anti-IL-17A administration. Besides, MAb anti-IFNγ greatly augmented transaminase plasmatic levels. IL-17A neutralization did not affect MHV-A59-induction of HMGB1 liberation and slightly augmented plasmatic uric acid concentration. However, mice treated with the MAb failed to produce autoAb to FAH. The above results suggest a reciprocal regulation of Th1 and Th17 cells acting on the different MHV-A59 effects. In addition, it is proposed that IL-17A is involved in alarmins adjuvant effects leading to autoAb expression.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47349
Aparicio, Jose Luis; Ottobre Saborido, Macarena Aylén; Duhalde Vega, Maite; Coutelier, Jean-Paul; Van Snick, Jacques ; et al.; Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection; John Libbey Eurotext Ltd; European Cytokine Network; 28; 3; 9-2017; 111-117
1148-5493
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47349
identifier_str_mv Aparicio, Jose Luis; Ottobre Saborido, Macarena Aylén; Duhalde Vega, Maite; Coutelier, Jean-Paul; Van Snick, Jacques ; et al.; Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection; John Libbey Eurotext Ltd; European Cytokine Network; 28; 3; 9-2017; 111-117
1148-5493
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jle.com/fr/revues/ecn/e-docs/effects_of_interleukin_17a_il_17a_neutralization_on_murine_hepatitis_virus_mhv_a59_infection_310871/article.phtml
info:eu-repo/semantics/altIdentifier/doi/10.1684/ecn.2017.0399
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Libbey Eurotext Ltd
publisher.none.fl_str_mv John Libbey Eurotext Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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