Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation

Autores
Zenker, Jennifer; White, Melanie D.; Gasnier, Maxime; Alvarez, Yanina Daniela; Lim, Hui Yi Grace; Bissiere, Stephanie; Biro, Maté; Plachta, Nicolas
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity.
Fil: Zenker, Jennifer. Institute Of Molecular And Cell Biology; Singapur
Fil: White, Melanie D.. Institute Of Molecular And Cell Biology; Singapur
Fil: Gasnier, Maxime. Institute Of Molecular And Cell Biology; Singapur
Fil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Lim, Hui Yi Grace. Institute Of Molecular And Cell Biology; Singapur
Fil: Bissiere, Stephanie. Institute Of Molecular And Cell Biology; Singapur
Fil: Biro, Maté. Institute Of Molecular And Cell Biology; Singapur
Fil: Plachta, Nicolas. Institute Of Molecular And Cell Biology; Singapur
Materia
ACTIN DYNAMICS
BLASTOCYST
CORTICAL FLOW
EPITHELIA
LIVE IMAGING
MAMMALIAN DEVELOPMENT
MICROTUBULES
MORPHOGENESIS
PREIMPLANTATION MOUSE EMBRYO
TIGHT JUNCTIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94046
Acceder
id CONICETDig_97d885e39a68b18e4056c673f5533ddc
oai_identifier_str oai:ri.conicet.gov.ar:11336/94046
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst FormationZenker, JenniferWhite, Melanie D.Gasnier, MaximeAlvarez, Yanina DanielaLim, Hui Yi GraceBissiere, StephanieBiro, MatéPlachta, NicolasACTIN DYNAMICSBLASTOCYSTCORTICAL FLOWEPITHELIALIVE IMAGINGMAMMALIAN DEVELOPMENTMICROTUBULESMORPHOGENESISPREIMPLANTATION MOUSE EMBRYOTIGHT JUNCTIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity.Fil: Zenker, Jennifer. Institute Of Molecular And Cell Biology; SingapurFil: White, Melanie D.. Institute Of Molecular And Cell Biology; SingapurFil: Gasnier, Maxime. Institute Of Molecular And Cell Biology; SingapurFil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Lim, Hui Yi Grace. Institute Of Molecular And Cell Biology; SingapurFil: Bissiere, Stephanie. Institute Of Molecular And Cell Biology; SingapurFil: Biro, Maté. Institute Of Molecular And Cell Biology; SingapurFil: Plachta, Nicolas. Institute Of Molecular And Cell Biology; SingapurCell Press2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94046Zenker, Jennifer; White, Melanie D.; Gasnier, Maxime; Alvarez, Yanina Daniela; Lim, Hui Yi Grace; et al.; Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation; Cell Press; Cell; 173; 3; 4-2018; 776-791.e170092-8674CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0092867418302125info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cell.2018.02.035info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:49:06Zoai:ri.conicet.gov.ar:11336/94046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:49:06.521CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
title Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
spellingShingle Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
Zenker, Jennifer
ACTIN DYNAMICS
BLASTOCYST
CORTICAL FLOW
EPITHELIA
LIVE IMAGING
MAMMALIAN DEVELOPMENT
MICROTUBULES
MORPHOGENESIS
PREIMPLANTATION MOUSE EMBRYO
TIGHT JUNCTIONS
title_short Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
title_full Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
title_fullStr Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
title_full_unstemmed Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
title_sort Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation
dc.creator.none.fl_str_mv Zenker, Jennifer
White, Melanie D.
Gasnier, Maxime
Alvarez, Yanina Daniela
Lim, Hui Yi Grace
Bissiere, Stephanie
Biro, Maté
Plachta, Nicolas
author Zenker, Jennifer
author_facet Zenker, Jennifer
White, Melanie D.
Gasnier, Maxime
Alvarez, Yanina Daniela
Lim, Hui Yi Grace
Bissiere, Stephanie
Biro, Maté
Plachta, Nicolas
author_role author
author2 White, Melanie D.
Gasnier, Maxime
Alvarez, Yanina Daniela
Lim, Hui Yi Grace
Bissiere, Stephanie
Biro, Maté
Plachta, Nicolas
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTIN DYNAMICS
BLASTOCYST
CORTICAL FLOW
EPITHELIA
LIVE IMAGING
MAMMALIAN DEVELOPMENT
MICROTUBULES
MORPHOGENESIS
PREIMPLANTATION MOUSE EMBRYO
TIGHT JUNCTIONS
topic ACTIN DYNAMICS
BLASTOCYST
CORTICAL FLOW
EPITHELIA
LIVE IMAGING
MAMMALIAN DEVELOPMENT
MICROTUBULES
MORPHOGENESIS
PREIMPLANTATION MOUSE EMBRYO
TIGHT JUNCTIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity.
Fil: Zenker, Jennifer. Institute Of Molecular And Cell Biology; Singapur
Fil: White, Melanie D.. Institute Of Molecular And Cell Biology; Singapur
Fil: Gasnier, Maxime. Institute Of Molecular And Cell Biology; Singapur
Fil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Lim, Hui Yi Grace. Institute Of Molecular And Cell Biology; Singapur
Fil: Bissiere, Stephanie. Institute Of Molecular And Cell Biology; Singapur
Fil: Biro, Maté. Institute Of Molecular And Cell Biology; Singapur
Fil: Plachta, Nicolas. Institute Of Molecular And Cell Biology; Singapur
description Transformation from morula to blastocyst is a defining event of preimplantation embryo development. During this transition, the embryo must establish a paracellular permeability barrier to enable expansion of the blastocyst cavity. Here, using live imaging of mouse embryos, we reveal an actin-zippering mechanism driving this embryo sealing. Preceding blastocyst stage, a cortical F-actin ring assembles at the apical pole of the embryo's outer cells. The ring structure forms when cortical actin flows encounter a network of polar microtubules that exclude F-actin. Unlike stereotypical actin rings, the actin rings of the mouse embryo are not contractile, but instead, they expand to the cell-cell junctions. Here, they couple to the junctions by recruiting and stabilizing adherens and tight junction components. Coupling of the actin rings triggers localized myosin II accumulation, and it initiates a tension-dependent zippering mechanism along the junctions that is required to seal the embryo for blastocyst formation. Expanding actin rings zipper the mouse embryo to seal it and allow formation of the blastocyst cavity.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94046
Zenker, Jennifer; White, Melanie D.; Gasnier, Maxime; Alvarez, Yanina Daniela; Lim, Hui Yi Grace; et al.; Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation; Cell Press; Cell; 173; 3; 4-2018; 776-791.e17
0092-8674
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94046
identifier_str_mv Zenker, Jennifer; White, Melanie D.; Gasnier, Maxime; Alvarez, Yanina Daniela; Lim, Hui Yi Grace; et al.; Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation; Cell Press; Cell; 173; 3; 4-2018; 776-791.e17
0092-8674
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0092867418302125
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cell.2018.02.035
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848598051046817792
score 12.976206

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