Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli

Autores
Hasperué, Héctor Joaquín; Gómez Lobato, María Eugenia; Chaves, Alicia Raquel; Civello, Pedro Marcos; Martinez, Gustavo A
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of the day at which the broccoli is harvested can influence in the rate of chlorophyll degradation. This report describes changes of expression of chlorophyll degrading related genes during postharvest storage of heads harvested at different moments of day. At harvest, the content of chlorophyll did not change among samples but the expression of many genes associated with chlorophyll degradation did. The level of chlorophyll diminished during storage, but samples harvested at 18:00 h had a lower rate of degradationincomparison withheadsharvestedat 08:00 h.Most of genes that werepreviously associated with chlorophyll degradation during senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed a lower expression or a delay in their mRNA level increments in samples harvested at 18:00 h. Other genes related to chlorophyll degradation during senescence, like BoNYC or BoRCCR, showed an increased expression during senescence but there was no positive correlation between chlorophyll catabolism and expression of these genes. In conclusion, changes in the metabolism due to time of day at harvest not only influence the expression of genes during the day but also may cause different patterns of expression during postharvest.
Fil: Hasperué, Héctor Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Chaves, Alicia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Martinez, Gustavo A. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Materia
Broccoli
Postharvest
Senescence
Daytime
Chlorophyll Degrading Genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10237
Acceder
id CONICETDig_0147dc95fb7d458270fee5180740c26d
oai_identifier_str oai:ri.conicet.gov.ar:11336/10237
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoliHasperué, Héctor JoaquínGómez Lobato, María EugeniaChaves, Alicia RaquelCivello, Pedro MarcosMartinez, Gustavo ABroccoliPostharvestSenescenceDaytimeChlorophyll Degrading Geneshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of the day at which the broccoli is harvested can influence in the rate of chlorophyll degradation. This report describes changes of expression of chlorophyll degrading related genes during postharvest storage of heads harvested at different moments of day. At harvest, the content of chlorophyll did not change among samples but the expression of many genes associated with chlorophyll degradation did. The level of chlorophyll diminished during storage, but samples harvested at 18:00 h had a lower rate of degradationincomparison withheadsharvestedat 08:00 h.Most of genes that werepreviously associated with chlorophyll degradation during senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed a lower expression or a delay in their mRNA level increments in samples harvested at 18:00 h. Other genes related to chlorophyll degradation during senescence, like BoNYC or BoRCCR, showed an increased expression during senescence but there was no positive correlation between chlorophyll catabolism and expression of these genes. In conclusion, changes in the metabolism due to time of day at harvest not only influence the expression of genes during the day but also may cause different patterns of expression during postharvest.Fil: Hasperué, Héctor Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Chaves, Alicia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Martinez, Gustavo A. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaElsevier Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10237Hasperué, Héctor Joaquín; Gómez Lobato, María Eugenia; Chaves, Alicia Raquel; Civello, Pedro Marcos; Martinez, Gustavo A; Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli; Elsevier Science; Postharvest Biology And Technology; 82; 8-2013; 22-270925-5214enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2013.02.021info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521413000653info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:38Zoai:ri.conicet.gov.ar:11336/10237instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:39.256CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
spellingShingle Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
Hasperué, Héctor Joaquín
Broccoli
Postharvest
Senescence
Daytime
Chlorophyll Degrading Genes
title_short Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_full Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_fullStr Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_full_unstemmed Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
title_sort Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli
dc.creator.none.fl_str_mv Hasperué, Héctor Joaquín
Gómez Lobato, María Eugenia
Chaves, Alicia Raquel
Civello, Pedro Marcos
Martinez, Gustavo A
author Hasperué, Héctor Joaquín
author_facet Hasperué, Héctor Joaquín
Gómez Lobato, María Eugenia
Chaves, Alicia Raquel
Civello, Pedro Marcos
Martinez, Gustavo A
author_role author
author2 Gómez Lobato, María Eugenia
Chaves, Alicia Raquel
Civello, Pedro Marcos
Martinez, Gustavo A
author2_role author
author
author
author
dc.subject.none.fl_str_mv Broccoli
Postharvest
Senescence
Daytime
Chlorophyll Degrading Genes
topic Broccoli
Postharvest
Senescence
Daytime
Chlorophyll Degrading Genes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of the day at which the broccoli is harvested can influence in the rate of chlorophyll degradation. This report describes changes of expression of chlorophyll degrading related genes during postharvest storage of heads harvested at different moments of day. At harvest, the content of chlorophyll did not change among samples but the expression of many genes associated with chlorophyll degradation did. The level of chlorophyll diminished during storage, but samples harvested at 18:00 h had a lower rate of degradationincomparison withheadsharvestedat 08:00 h.Most of genes that werepreviously associated with chlorophyll degradation during senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed a lower expression or a delay in their mRNA level increments in samples harvested at 18:00 h. Other genes related to chlorophyll degradation during senescence, like BoNYC or BoRCCR, showed an increased expression during senescence but there was no positive correlation between chlorophyll catabolism and expression of these genes. In conclusion, changes in the metabolism due to time of day at harvest not only influence the expression of genes during the day but also may cause different patterns of expression during postharvest.
Fil: Hasperué, Héctor Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Gómez Lobato, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Chaves, Alicia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Martinez, Gustavo A. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
description Broccoli is an important component of the human diet with a high nutritional value. This vegetable is harvested when its development is not complete, triggering a quick senescence that is accompanied by chlorophyll degradation and a shortening of shelf life. Recently it was shown that the time of the day at which the broccoli is harvested can influence in the rate of chlorophyll degradation. This report describes changes of expression of chlorophyll degrading related genes during postharvest storage of heads harvested at different moments of day. At harvest, the content of chlorophyll did not change among samples but the expression of many genes associated with chlorophyll degradation did. The level of chlorophyll diminished during storage, but samples harvested at 18:00 h had a lower rate of degradationincomparison withheadsharvestedat 08:00 h.Most of genes that werepreviously associated with chlorophyll degradation during senescence such as BoSGR, BoCLH2, BoPPH and BoPaO showed a lower expression or a delay in their mRNA level increments in samples harvested at 18:00 h. Other genes related to chlorophyll degradation during senescence, like BoNYC or BoRCCR, showed an increased expression during senescence but there was no positive correlation between chlorophyll catabolism and expression of these genes. In conclusion, changes in the metabolism due to time of day at harvest not only influence the expression of genes during the day but also may cause different patterns of expression during postharvest.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10237
Hasperué, Héctor Joaquín; Gómez Lobato, María Eugenia; Chaves, Alicia Raquel; Civello, Pedro Marcos; Martinez, Gustavo A; Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli; Elsevier Science; Postharvest Biology And Technology; 82; 8-2013; 22-27
0925-5214
url http://hdl.handle.net/11336/10237
identifier_str_mv Hasperué, Héctor Joaquín; Gómez Lobato, María Eugenia; Chaves, Alicia Raquel; Civello, Pedro Marcos; Martinez, Gustavo A; Time of day at harvest affects the expression of chlorophyll degrading genes during postharvest storage of broccoli; Elsevier Science; Postharvest Biology And Technology; 82; 8-2013; 22-27
0925-5214
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2013.02.021
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521413000653
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270053752373248
score 13.13397

Publicaciones similares