Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apopt...
- Autores
- Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; Bondt, Albert; Wuhrer, Manfred; Costa, Serban Dan; Zenclussen, Ana Claudia
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins.
Fil: Fettke, Franziska. Otto-von-Guericke-Universität Magdeburg; Alemania
Fil: Schumacher, Anne. Otto-von-Guericke-Universität Magdeburg; Alemania
Fil: Canellada, Andrea Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Toledo, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Bekeredjian Ding, Isabelle. Paul-Ehrlich-Institute; Alemania
Fil: Bondt, Albert. Leiden University; Países Bajos
Fil: Wuhrer, Manfred. Leiden University; Países Bajos
Fil: Costa, Serban Dan. Otto-von-Guericke University. University Women’s Clinic; Alemania
Fil: Zenclussen, Ana Claudia. Otto-von-Guericke-Universität Magdeburg; Alemania - Materia
-
AFP
B CELLS
HCG
HORMONES
IL-10
PLACENTA
PREGNANCY
TOLERANCE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/59265
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Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosisFettke, FranziskaSchumacher, AnneCanellada, Andrea MercedesToledo, NataliaBekeredjian Ding, IsabelleBondt, AlbertWuhrer, ManfredCosta, Serban DanZenclussen, Ana ClaudiaAFPB CELLSHCGHORMONESIL-10PLACENTAPREGNANCYTOLERANCEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins.Fil: Fettke, Franziska. Otto-von-Guericke-Universität Magdeburg; AlemaniaFil: Schumacher, Anne. Otto-von-Guericke-Universität Magdeburg; AlemaniaFil: Canellada, Andrea Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Toledo, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Bekeredjian Ding, Isabelle. Paul-Ehrlich-Institute; AlemaniaFil: Bondt, Albert. Leiden University; Países BajosFil: Wuhrer, Manfred. Leiden University; Países BajosFil: Costa, Serban Dan. Otto-von-Guericke University. University Women’s Clinic; AlemaniaFil: Zenclussen, Ana Claudia. Otto-von-Guericke-Universität Magdeburg; AlemaniaFrontiers Research Foundation2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/59265Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; et al.; Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis; Frontiers Research Foundation; Frontiers in Immunology; 7; DEC; 10-2016; 1-131664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2016.00495info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2016.00495/fullinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144100/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:18Zoai:ri.conicet.gov.ar:11336/59265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:19.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
title |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
spellingShingle |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis Fettke, Franziska AFP B CELLS HCG HORMONES IL-10 PLACENTA PREGNANCY TOLERANCE |
title_short |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
title_full |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
title_fullStr |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
title_full_unstemmed |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
title_sort |
Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis |
dc.creator.none.fl_str_mv |
Fettke, Franziska Schumacher, Anne Canellada, Andrea Mercedes Toledo, Natalia Bekeredjian Ding, Isabelle Bondt, Albert Wuhrer, Manfred Costa, Serban Dan Zenclussen, Ana Claudia |
author |
Fettke, Franziska |
author_facet |
Fettke, Franziska Schumacher, Anne Canellada, Andrea Mercedes Toledo, Natalia Bekeredjian Ding, Isabelle Bondt, Albert Wuhrer, Manfred Costa, Serban Dan Zenclussen, Ana Claudia |
author_role |
author |
author2 |
Schumacher, Anne Canellada, Andrea Mercedes Toledo, Natalia Bekeredjian Ding, Isabelle Bondt, Albert Wuhrer, Manfred Costa, Serban Dan Zenclussen, Ana Claudia |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
AFP B CELLS HCG HORMONES IL-10 PLACENTA PREGNANCY TOLERANCE |
topic |
AFP B CELLS HCG HORMONES IL-10 PLACENTA PREGNANCY TOLERANCE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins. Fil: Fettke, Franziska. Otto-von-Guericke-Universität Magdeburg; Alemania Fil: Schumacher, Anne. Otto-von-Guericke-Universität Magdeburg; Alemania Fil: Canellada, Andrea Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina Fil: Toledo, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina Fil: Bekeredjian Ding, Isabelle. Paul-Ehrlich-Institute; Alemania Fil: Bondt, Albert. Leiden University; Países Bajos Fil: Wuhrer, Manfred. Leiden University; Países Bajos Fil: Costa, Serban Dan. Otto-von-Guericke University. University Women’s Clinic; Alemania Fil: Zenclussen, Ana Claudia. Otto-von-Guericke-Universität Magdeburg; Alemania |
description |
Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/59265 Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; et al.; Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis; Frontiers Research Foundation; Frontiers in Immunology; 7; DEC; 10-2016; 1-13 1664-3224 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/59265 |
identifier_str_mv |
Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; et al.; Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis; Frontiers Research Foundation; Frontiers in Immunology; 7; DEC; 10-2016; 1-13 1664-3224 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2016.00495 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2016.00495/full info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144100/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/zip application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Research Foundation |
publisher.none.fl_str_mv |
Frontiers Research Foundation |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.22299 |