Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apopt...

Autores
Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; Bondt, Albert; Wuhrer, Manfred; Costa, Serban Dan; Zenclussen, Ana Claudia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins.
Fil: Fettke, Franziska. Otto-von-Guericke-Universität Magdeburg; Alemania
Fil: Schumacher, Anne. Otto-von-Guericke-Universität Magdeburg; Alemania
Fil: Canellada, Andrea Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Toledo, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Bekeredjian Ding, Isabelle. Paul-Ehrlich-Institute; Alemania
Fil: Bondt, Albert. Leiden University; Países Bajos
Fil: Wuhrer, Manfred. Leiden University; Países Bajos
Fil: Costa, Serban Dan. Otto-von-Guericke University. University Women’s Clinic; Alemania
Fil: Zenclussen, Ana Claudia. Otto-von-Guericke-Universität Magdeburg; Alemania
Materia
AFP
B CELLS
HCG
HORMONES
IL-10
PLACENTA
PREGNANCY
TOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59265

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosisFettke, FranziskaSchumacher, AnneCanellada, Andrea MercedesToledo, NataliaBekeredjian Ding, IsabelleBondt, AlbertWuhrer, ManfredCosta, Serban DanZenclussen, Ana ClaudiaAFPB CELLSHCGHORMONESIL-10PLACENTAPREGNANCYTOLERANCEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins.Fil: Fettke, Franziska. Otto-von-Guericke-Universität Magdeburg; AlemaniaFil: Schumacher, Anne. Otto-von-Guericke-Universität Magdeburg; AlemaniaFil: Canellada, Andrea Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Toledo, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Bekeredjian Ding, Isabelle. Paul-Ehrlich-Institute; AlemaniaFil: Bondt, Albert. Leiden University; Países BajosFil: Wuhrer, Manfred. Leiden University; Países BajosFil: Costa, Serban Dan. Otto-von-Guericke University. University Women’s Clinic; AlemaniaFil: Zenclussen, Ana Claudia. Otto-von-Guericke-Universität Magdeburg; AlemaniaFrontiers Research Foundation2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/59265Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; et al.; Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis; Frontiers Research Foundation; Frontiers in Immunology; 7; DEC; 10-2016; 1-131664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2016.00495info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2016.00495/fullinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144100/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:18Zoai:ri.conicet.gov.ar:11336/59265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:19.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
title Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
spellingShingle Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
Fettke, Franziska
AFP
B CELLS
HCG
HORMONES
IL-10
PLACENTA
PREGNANCY
TOLERANCE
title_short Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
title_full Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
title_fullStr Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
title_full_unstemmed Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
title_sort Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis
dc.creator.none.fl_str_mv Fettke, Franziska
Schumacher, Anne
Canellada, Andrea Mercedes
Toledo, Natalia
Bekeredjian Ding, Isabelle
Bondt, Albert
Wuhrer, Manfred
Costa, Serban Dan
Zenclussen, Ana Claudia
author Fettke, Franziska
author_facet Fettke, Franziska
Schumacher, Anne
Canellada, Andrea Mercedes
Toledo, Natalia
Bekeredjian Ding, Isabelle
Bondt, Albert
Wuhrer, Manfred
Costa, Serban Dan
Zenclussen, Ana Claudia
author_role author
author2 Schumacher, Anne
Canellada, Andrea Mercedes
Toledo, Natalia
Bekeredjian Ding, Isabelle
Bondt, Albert
Wuhrer, Manfred
Costa, Serban Dan
Zenclussen, Ana Claudia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AFP
B CELLS
HCG
HORMONES
IL-10
PLACENTA
PREGNANCY
TOLERANCE
topic AFP
B CELLS
HCG
HORMONES
IL-10
PLACENTA
PREGNANCY
TOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins.
Fil: Fettke, Franziska. Otto-von-Guericke-Universität Magdeburg; Alemania
Fil: Schumacher, Anne. Otto-von-Guericke-Universität Magdeburg; Alemania
Fil: Canellada, Andrea Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Toledo, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Bekeredjian Ding, Isabelle. Paul-Ehrlich-Institute; Alemania
Fil: Bondt, Albert. Leiden University; Países Bajos
Fil: Wuhrer, Manfred. Leiden University; Países Bajos
Fil: Costa, Serban Dan. Otto-von-Guericke University. University Women’s Clinic; Alemania
Fil: Zenclussen, Ana Claudia. Otto-von-Guericke-Universität Magdeburg; Alemania
description Maternal immune tolerance toward the fetus is an essential requisite for pregnancy. While T cell functions are well documented, little is known about the participation of B cells. We have previously suggested that IL-10-producing B cells are involved in pregnancy tolerance in mice and humans. By employing murine and human systems, we report now that fetal trophoblasts positively regulate the generation of IL-10-producing B cells. We next studied the participation of hormones produced by the placenta as well as the fetal protein alpha-fetoprotein (AFP) in B cell modulation. Human chorionic gonadotropin (hCG), but not progesterone, estrogen, or a combination of both, was able to promote changes in B cell phenotype and boost their IL-10 production, which was abolished after blocking hCG. The hCG-induced B cell phenotype was not associated with augmented galactosylation, sialylation, or fucosylation of IgG subclasses in their Fc. In vitro, hCG induced the synthesis of asymmetrically glycosylated antibodies in their Fab region. Interestingly, AFP had dual effects depending on the concentration. At concentrations corresponding to maternal serum levels, it did not modify the phenotype or IL-10 secretion of B cells. At fetal concentrations, however, AFP was able to drive B cells into apoptosis, which may indicate a protective mechanism to avoid maternal B cells to reach the fetus. Our data suggest that the fetus secrete factors that promote a pregnancy-friendly B cell phenotype, unraveling interesting aspects of B cell function, and modulation by pregnancy hormones and fetal proteins.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59265
Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; et al.; Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis; Frontiers Research Foundation; Frontiers in Immunology; 7; DEC; 10-2016; 1-13
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59265
identifier_str_mv Fettke, Franziska; Schumacher, Anne; Canellada, Andrea Mercedes; Toledo, Natalia; Bekeredjian Ding, Isabelle; et al.; Maternal and fetal mechanisms of B cell regulation during pregnancy: Human chorionic gonadotropin stimulates B cells to produce IL-10 while alpha-fetoprotein drives them into apoptosis; Frontiers Research Foundation; Frontiers in Immunology; 7; DEC; 10-2016; 1-13
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2016.00495
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2016.00495/full
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144100/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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