Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium

Autores
Riggs, Ryan M.; Bocca, Silvina; Anderson, Sandra; Franchi, Nilda Anahi; Rhavi, Bhaskara S.; Oehninger, Sergio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule-epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression. Design: Primary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs). Setting: Academic center. Patient(s): Ovulatory women aged 21-30 years. Intervention(s): Treatment with MFG-E8 and hCG. Main Outcome Measure(s): Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription-polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins. Result(s): Endometrial epithelial cells were cytokeratin+, vimentin-, MFG-E8+, and hCG-R+, whereas ESC were vimentin+, cytokeratin -, MFG-E8-, and hCG-R+. Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC. Conclusion(s): Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells.
Fil: Riggs, Ryan M.. Eastern Virginia Medical School; Estados Unidos
Fil: Bocca, Silvina. Eastern Virginia Medical School; Estados Unidos
Fil: Anderson, Sandra. Eastern Virginia Medical School; Estados Unidos
Fil: Franchi, Nilda Anahi. Eastern Virginia Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rhavi, Bhaskara S.. Old Dominion University; Estados Unidos
Fil: Oehninger, Sergio. Eastern Virginia Medical School; Estados Unidos
Materia
Apoptosis
Caspase Activity
Endometrium
Hcg
Human
Mfg-E8
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76768
Acceder
id CONICETDig_08d2e866fc778871fb687ee2a0bc6b5b
oai_identifier_str oai:ri.conicet.gov.ar:11336/76768
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometriumRiggs, Ryan M.Bocca, SilvinaAnderson, SandraFranchi, Nilda AnahiRhavi, Bhaskara S.Oehninger, SergioApoptosisCaspase ActivityEndometriumHcgHumanMfg-E8https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule-epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression. Design: Primary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs). Setting: Academic center. Patient(s): Ovulatory women aged 21-30 years. Intervention(s): Treatment with MFG-E8 and hCG. Main Outcome Measure(s): Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription-polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins. Result(s): Endometrial epithelial cells were cytokeratin+, vimentin-, MFG-E8+, and hCG-R+, whereas ESC were vimentin+, cytokeratin -, MFG-E8-, and hCG-R+. Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC. Conclusion(s): Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells.Fil: Riggs, Ryan M.. Eastern Virginia Medical School; Estados UnidosFil: Bocca, Silvina. Eastern Virginia Medical School; Estados UnidosFil: Anderson, Sandra. Eastern Virginia Medical School; Estados UnidosFil: Franchi, Nilda Anahi. Eastern Virginia Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Rhavi, Bhaskara S.. Old Dominion University; Estados UnidosFil: Oehninger, Sergio. Eastern Virginia Medical School; Estados UnidosElsevier Science Inc2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76768Riggs, Ryan M.; Bocca, Silvina; Anderson, Sandra; Franchi, Nilda Anahi; Rhavi, Bhaskara S.; et al.; Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium; Elsevier Science Inc; Fertility and Sterility; 98; 6; 12-20120015-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22921913info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2012.07.1127info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:06Zoai:ri.conicet.gov.ar:11336/76768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:06.317CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
title Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
spellingShingle Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
Riggs, Ryan M.
Apoptosis
Caspase Activity
Endometrium
Hcg
Human
Mfg-E8
title_short Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
title_full Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
title_fullStr Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
title_full_unstemmed Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
title_sort Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium
dc.creator.none.fl_str_mv Riggs, Ryan M.
Bocca, Silvina
Anderson, Sandra
Franchi, Nilda Anahi
Rhavi, Bhaskara S.
Oehninger, Sergio
author Riggs, Ryan M.
author_facet Riggs, Ryan M.
Bocca, Silvina
Anderson, Sandra
Franchi, Nilda Anahi
Rhavi, Bhaskara S.
Oehninger, Sergio
author_role author
author2 Bocca, Silvina
Anderson, Sandra
Franchi, Nilda Anahi
Rhavi, Bhaskara S.
Oehninger, Sergio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Caspase Activity
Endometrium
Hcg
Human
Mfg-E8
topic Apoptosis
Caspase Activity
Endometrium
Hcg
Human
Mfg-E8
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule-epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression. Design: Primary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs). Setting: Academic center. Patient(s): Ovulatory women aged 21-30 years. Intervention(s): Treatment with MFG-E8 and hCG. Main Outcome Measure(s): Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription-polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins. Result(s): Endometrial epithelial cells were cytokeratin+, vimentin-, MFG-E8+, and hCG-R+, whereas ESC were vimentin+, cytokeratin -, MFG-E8-, and hCG-R+. Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC. Conclusion(s): Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells.
Fil: Riggs, Ryan M.. Eastern Virginia Medical School; Estados Unidos
Fil: Bocca, Silvina. Eastern Virginia Medical School; Estados Unidos
Fil: Anderson, Sandra. Eastern Virginia Medical School; Estados Unidos
Fil: Franchi, Nilda Anahi. Eastern Virginia Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rhavi, Bhaskara S.. Old Dominion University; Estados Unidos
Fil: Oehninger, Sergio. Eastern Virginia Medical School; Estados Unidos
description To study the regulation of apoptosis in human endometrial cells. The specific aims were to determine whether milk fat globule-epidermal growth factor 8 (MFG-E8), a novel endometrial epithelial protein, modulates caspase activation and DNA fragmentation; and to examine whether hCG, an early embryonic product, regulates Bax and Bcl-2 equilibrium, as well as MFG-E8 expression. Design: Primary cultures of human endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs). Setting: Academic center. Patient(s): Ovulatory women aged 21-30 years. Intervention(s): Treatment with MFG-E8 and hCG. Main Outcome Measure(s): Apoptotic activity was quantified using a luciferase assay. Deoxyribonucleic acid fragmentation was detected by TUNEL assay. Bax, Bcl-2, and MFG-E8 messenger RNA expression levels were determined by quantitative reverse transcription-polymerase chain reaction. Immunocytochemistry was used to establish cell purity and presence of MFG-E8 and hCG-R (receptor) proteins. Result(s): Endometrial epithelial cells were cytokeratin+, vimentin-, MFG-E8+, and hCG-R+, whereas ESC were vimentin+, cytokeratin -, MFG-E8-, and hCG-R+. Treatment of ESC with MFG-E8 resulted in a 13-fold increase in caspase activity and a 30-fold increase in TUNEL. On the other hand, hCG decreased messenger RNA expression of Bax in ESC. Conclusion(s): Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. Conversely, pregnancy levels of hCG has opposite effects on stromal cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76768
Riggs, Ryan M.; Bocca, Silvina; Anderson, Sandra; Franchi, Nilda Anahi; Rhavi, Bhaskara S.; et al.; Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium; Elsevier Science Inc; Fertility and Sterility; 98; 6; 12-2012
0015-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76768
identifier_str_mv Riggs, Ryan M.; Bocca, Silvina; Anderson, Sandra; Franchi, Nilda Anahi; Rhavi, Bhaskara S.; et al.; Epithelial cell protein milk fat globule-epidermal growth factor 8 and human chorionic gonadotropin regulate stromal cell apoptosis in the human endometrium; Elsevier Science Inc; Fertility and Sterility; 98; 6; 12-2012
0015-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22921913
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fertnstert.2012.07.1127
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083500239749120
score 13.22299

Publicaciones similares