Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)

Autores
Blanco Cohene, Tania Katherina; Olea, Gabriela Beatriz; Flores Quintana, Carolina Isabel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study aims to structurally and histochemically characterize the Gymnotus carapo tegument. 30 specimens were captured and slaughtered by spinal section with anesthesia. The observation was carried out with a stereoscopic microscope and the body surface was photographed. Fragments of the dorsal, ventral and lateral region were fixed in Bouin’s solution for 12 hours and subsequently preserved in 70% alcohol. They were subsequently observed in the scanning electron microscope (SEM). The preparation for SEM was performed following the standardized protocol. Histological preparations were made, and the cuts were colored with H-E, PAS and Coomassie Blue. The images were obtained in an Olympus BX41-ENUTV-4 microscope. From the observations in SEM a plain tegument with pores of different sizes could be evidenced. The scales of the different regions of the body have different ornaments. Microscopically it was composed of a stratified non-keratinized epithelium consisting of two types of morphologically distinct cells: epidermal cells and mucous cells (PAS-Commassie Blue positive). Under the epithelium there is a layer of dense irregular connective tissue with associated chromatophores and more deeply scales. These analyzes are the basis for future studies that will focus on elucidating the events related to integumentary healing in this species.
Fil: Blanco Cohene, Tania Katherina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina
Fil: Olea, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina. Universidad Nacional del Chaco Austral; Argentina
Fil: Flores Quintana, Carolina Isabel. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina. Universidad Nacional del Chaco Austral; Argentina
Materia
DERMIS
EPIDERMIS
FISH
MUCOUS GLANDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/223656
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)Blanco Cohene, Tania KatherinaOlea, Gabriela BeatrizFlores Quintana, Carolina IsabelDERMISEPIDERMISFISHMUCOUS GLANDShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The present study aims to structurally and histochemically characterize the Gymnotus carapo tegument. 30 specimens were captured and slaughtered by spinal section with anesthesia. The observation was carried out with a stereoscopic microscope and the body surface was photographed. Fragments of the dorsal, ventral and lateral region were fixed in Bouin’s solution for 12 hours and subsequently preserved in 70% alcohol. They were subsequently observed in the scanning electron microscope (SEM). The preparation for SEM was performed following the standardized protocol. Histological preparations were made, and the cuts were colored with H-E, PAS and Coomassie Blue. The images were obtained in an Olympus BX41-ENUTV-4 microscope. From the observations in SEM a plain tegument with pores of different sizes could be evidenced. The scales of the different regions of the body have different ornaments. Microscopically it was composed of a stratified non-keratinized epithelium consisting of two types of morphologically distinct cells: epidermal cells and mucous cells (PAS-Commassie Blue positive). Under the epithelium there is a layer of dense irregular connective tissue with associated chromatophores and more deeply scales. These analyzes are the basis for future studies that will focus on elucidating the events related to integumentary healing in this species.Fil: Blanco Cohene, Tania Katherina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; ArgentinaFil: Olea, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina. Universidad Nacional del Chaco Austral; ArgentinaFil: Flores Quintana, Carolina Isabel. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina. Universidad Nacional del Chaco Austral; ArgentinaAcademia Brasileira de Ciencias2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223656Blanco Cohene, Tania Katherina; Olea, Gabriela Beatriz; Flores Quintana, Carolina Isabel; Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 95; 4; 7-2023; 1-100001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/bzWfhRwBJg3Mbt3485qZT8g/?lang=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202320191259info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:30Zoai:ri.conicet.gov.ar:11336/223656instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:30.975CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
title Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
spellingShingle Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
Blanco Cohene, Tania Katherina
DERMIS
EPIDERMIS
FISH
MUCOUS GLANDS
title_short Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
title_full Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
title_fullStr Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
title_full_unstemmed Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
title_sort Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)
dc.creator.none.fl_str_mv Blanco Cohene, Tania Katherina
Olea, Gabriela Beatriz
Flores Quintana, Carolina Isabel
author Blanco Cohene, Tania Katherina
author_facet Blanco Cohene, Tania Katherina
Olea, Gabriela Beatriz
Flores Quintana, Carolina Isabel
author_role author
author2 Olea, Gabriela Beatriz
Flores Quintana, Carolina Isabel
author2_role author
author
dc.subject.none.fl_str_mv DERMIS
EPIDERMIS
FISH
MUCOUS GLANDS
topic DERMIS
EPIDERMIS
FISH
MUCOUS GLANDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The present study aims to structurally and histochemically characterize the Gymnotus carapo tegument. 30 specimens were captured and slaughtered by spinal section with anesthesia. The observation was carried out with a stereoscopic microscope and the body surface was photographed. Fragments of the dorsal, ventral and lateral region were fixed in Bouin’s solution for 12 hours and subsequently preserved in 70% alcohol. They were subsequently observed in the scanning electron microscope (SEM). The preparation for SEM was performed following the standardized protocol. Histological preparations were made, and the cuts were colored with H-E, PAS and Coomassie Blue. The images were obtained in an Olympus BX41-ENUTV-4 microscope. From the observations in SEM a plain tegument with pores of different sizes could be evidenced. The scales of the different regions of the body have different ornaments. Microscopically it was composed of a stratified non-keratinized epithelium consisting of two types of morphologically distinct cells: epidermal cells and mucous cells (PAS-Commassie Blue positive). Under the epithelium there is a layer of dense irregular connective tissue with associated chromatophores and more deeply scales. These analyzes are the basis for future studies that will focus on elucidating the events related to integumentary healing in this species.
Fil: Blanco Cohene, Tania Katherina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina
Fil: Olea, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina. Universidad Nacional del Chaco Austral; Argentina
Fil: Flores Quintana, Carolina Isabel. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Cs.basicas. Cátedra de Histología y Embriologia; Argentina. Universidad Nacional del Chaco Austral; Argentina
description The present study aims to structurally and histochemically characterize the Gymnotus carapo tegument. 30 specimens were captured and slaughtered by spinal section with anesthesia. The observation was carried out with a stereoscopic microscope and the body surface was photographed. Fragments of the dorsal, ventral and lateral region were fixed in Bouin’s solution for 12 hours and subsequently preserved in 70% alcohol. They were subsequently observed in the scanning electron microscope (SEM). The preparation for SEM was performed following the standardized protocol. Histological preparations were made, and the cuts were colored with H-E, PAS and Coomassie Blue. The images were obtained in an Olympus BX41-ENUTV-4 microscope. From the observations in SEM a plain tegument with pores of different sizes could be evidenced. The scales of the different regions of the body have different ornaments. Microscopically it was composed of a stratified non-keratinized epithelium consisting of two types of morphologically distinct cells: epidermal cells and mucous cells (PAS-Commassie Blue positive). Under the epithelium there is a layer of dense irregular connective tissue with associated chromatophores and more deeply scales. These analyzes are the basis for future studies that will focus on elucidating the events related to integumentary healing in this species.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223656
Blanco Cohene, Tania Katherina; Olea, Gabriela Beatriz; Flores Quintana, Carolina Isabel; Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 95; 4; 7-2023; 1-10
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223656
identifier_str_mv Blanco Cohene, Tania Katherina; Olea, Gabriela Beatriz; Flores Quintana, Carolina Isabel; Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 95; 4; 7-2023; 1-10
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aabc/a/bzWfhRwBJg3Mbt3485qZT8g/?lang=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202320191259
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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