Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus

Autores
Rodrigues dos Santos, Julio; Effting, Carmeane; Serbent, María Pilar; Matsuda, Josie Budag
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study evaluated the viability of the bacterium Bacillus pumilus as a biological agent in the process of calcium carbonate precipitation on a concrete surface. This evaluation was carried out in a curing tank of concrete samples, applying a nutrient solution enriched with B. pumilus for 48 h. During the experimental period, a urease test was performed to determine whether the microorganisms could hydrolyze urea by the action of the urease enzyme. The results revealed that B. pumilus is susceptible to converting urea into ammonium and increasing the medium pH. There was also a 0.03 g cm-2 reduction in water absorption by capillarity in specimens biomineralized with B. pumilus compared to conventional specimens. Due to the degree of surface protection, microorganisms have reduced the material’s porosity, causing an increase in tensile strength by diametric compression of approximately 9.0 MPa. The lower height of capillary rise observed was 1.83 cm in biomineralized specimens and 3.83 cm for conventional specimens. The results obtained with the scanning electron microscope and energy dispersive spectroscopy indicate the presence of CaCO3 precipitated by the bacteria. In general, the results obtained in this study show that B. pumilus may improve its mechanical properties when it is applied superficially to concrete.
Fil: Rodrigues dos Santos, Julio. Universidade Federal de Santa Catarina; Brasil
Fil: Effting, Carmeane. Universidade Federal de Santa Catarina; Brasil
Fil: Serbent, María Pilar. Universidade Federal de Santa Catarina; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigación y Tecnología Química. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina
Fil: Matsuda, Josie Budag. Centro Universitário para o Desenvolvimento do Alto Vale do Itajaí; Brasil
Materia
SELF-HEALING
BACILLUS PUMILUS
CALCIUM CARBONATE
BIOMINERALIZATION
BIOCONCRETE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229943
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/229943
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilusBiomineralização de carbonato de cálcio no concreto pela ação do Bacillus pumilusRodrigues dos Santos, JulioEffting, CarmeaneSerbent, María PilarMatsuda, Josie BudagSELF-HEALINGBACILLUS PUMILUSCALCIUM CARBONATEBIOMINERALIZATIONBIOCONCRETEhttps://purl.org/becyt/ford/2.1https://purl.org/becyt/ford/2The present study evaluated the viability of the bacterium Bacillus pumilus as a biological agent in the process of calcium carbonate precipitation on a concrete surface. This evaluation was carried out in a curing tank of concrete samples, applying a nutrient solution enriched with B. pumilus for 48 h. During the experimental period, a urease test was performed to determine whether the microorganisms could hydrolyze urea by the action of the urease enzyme. The results revealed that B. pumilus is susceptible to converting urea into ammonium and increasing the medium pH. There was also a 0.03 g cm-2 reduction in water absorption by capillarity in specimens biomineralized with B. pumilus compared to conventional specimens. Due to the degree of surface protection, microorganisms have reduced the material’s porosity, causing an increase in tensile strength by diametric compression of approximately 9.0 MPa. The lower height of capillary rise observed was 1.83 cm in biomineralized specimens and 3.83 cm for conventional specimens. The results obtained with the scanning electron microscope and energy dispersive spectroscopy indicate the presence of CaCO3 precipitated by the bacteria. In general, the results obtained in this study show that B. pumilus may improve its mechanical properties when it is applied superficially to concrete.Fil: Rodrigues dos Santos, Julio. Universidade Federal de Santa Catarina; BrasilFil: Effting, Carmeane. Universidade Federal de Santa Catarina; BrasilFil: Serbent, María Pilar. Universidade Federal de Santa Catarina; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigación y Tecnología Química. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; ArgentinaFil: Matsuda, Josie Budag. Centro Universitário para o Desenvolvimento do Alto Vale do Itajaí; BrasilAssoc Brasileira Engenharia Sanitaria Ambiental2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229943Rodrigues dos Santos, Julio; Effting, Carmeane; Serbent, María Pilar; Matsuda, Josie Budag; Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus; Assoc Brasileira Engenharia Sanitaria Ambiental; Engenharia Sanitaria E Ambiental; 28; 10-2023; 1-81413-41521809-4457CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-41522023000100138&tlng=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/s1413-415220220277info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:36:48Zoai:ri.conicet.gov.ar:11336/229943instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:36:48.299CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
Biomineralização de carbonato de cálcio no concreto pela ação do Bacillus pumilus
title Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
spellingShingle Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
Rodrigues dos Santos, Julio
SELF-HEALING
BACILLUS PUMILUS
CALCIUM CARBONATE
BIOMINERALIZATION
BIOCONCRETE
title_short Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
title_full Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
title_fullStr Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
title_full_unstemmed Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
title_sort Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus
dc.creator.none.fl_str_mv Rodrigues dos Santos, Julio
Effting, Carmeane
Serbent, María Pilar
Matsuda, Josie Budag
author Rodrigues dos Santos, Julio
author_facet Rodrigues dos Santos, Julio
Effting, Carmeane
Serbent, María Pilar
Matsuda, Josie Budag
author_role author
author2 Effting, Carmeane
Serbent, María Pilar
Matsuda, Josie Budag
author2_role author
author
author
dc.subject.none.fl_str_mv SELF-HEALING
BACILLUS PUMILUS
CALCIUM CARBONATE
BIOMINERALIZATION
BIOCONCRETE
topic SELF-HEALING
BACILLUS PUMILUS
CALCIUM CARBONATE
BIOMINERALIZATION
BIOCONCRETE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.1
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The present study evaluated the viability of the bacterium Bacillus pumilus as a biological agent in the process of calcium carbonate precipitation on a concrete surface. This evaluation was carried out in a curing tank of concrete samples, applying a nutrient solution enriched with B. pumilus for 48 h. During the experimental period, a urease test was performed to determine whether the microorganisms could hydrolyze urea by the action of the urease enzyme. The results revealed that B. pumilus is susceptible to converting urea into ammonium and increasing the medium pH. There was also a 0.03 g cm-2 reduction in water absorption by capillarity in specimens biomineralized with B. pumilus compared to conventional specimens. Due to the degree of surface protection, microorganisms have reduced the material’s porosity, causing an increase in tensile strength by diametric compression of approximately 9.0 MPa. The lower height of capillary rise observed was 1.83 cm in biomineralized specimens and 3.83 cm for conventional specimens. The results obtained with the scanning electron microscope and energy dispersive spectroscopy indicate the presence of CaCO3 precipitated by the bacteria. In general, the results obtained in this study show that B. pumilus may improve its mechanical properties when it is applied superficially to concrete.
Fil: Rodrigues dos Santos, Julio. Universidade Federal de Santa Catarina; Brasil
Fil: Effting, Carmeane. Universidade Federal de Santa Catarina; Brasil
Fil: Serbent, María Pilar. Universidade Federal de Santa Catarina; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigación y Tecnología Química. Universidad Tecnológica Nacional. Facultad Regional Córdoba. Centro de Investigación y Tecnología Química; Argentina
Fil: Matsuda, Josie Budag. Centro Universitário para o Desenvolvimento do Alto Vale do Itajaí; Brasil
description The present study evaluated the viability of the bacterium Bacillus pumilus as a biological agent in the process of calcium carbonate precipitation on a concrete surface. This evaluation was carried out in a curing tank of concrete samples, applying a nutrient solution enriched with B. pumilus for 48 h. During the experimental period, a urease test was performed to determine whether the microorganisms could hydrolyze urea by the action of the urease enzyme. The results revealed that B. pumilus is susceptible to converting urea into ammonium and increasing the medium pH. There was also a 0.03 g cm-2 reduction in water absorption by capillarity in specimens biomineralized with B. pumilus compared to conventional specimens. Due to the degree of surface protection, microorganisms have reduced the material’s porosity, causing an increase in tensile strength by diametric compression of approximately 9.0 MPa. The lower height of capillary rise observed was 1.83 cm in biomineralized specimens and 3.83 cm for conventional specimens. The results obtained with the scanning electron microscope and energy dispersive spectroscopy indicate the presence of CaCO3 precipitated by the bacteria. In general, the results obtained in this study show that B. pumilus may improve its mechanical properties when it is applied superficially to concrete.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229943
Rodrigues dos Santos, Julio; Effting, Carmeane; Serbent, María Pilar; Matsuda, Josie Budag; Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus; Assoc Brasileira Engenharia Sanitaria Ambiental; Engenharia Sanitaria E Ambiental; 28; 10-2023; 1-8
1413-4152
1809-4457
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229943
identifier_str_mv Rodrigues dos Santos, Julio; Effting, Carmeane; Serbent, María Pilar; Matsuda, Josie Budag; Biomineralization of calcium carbonate in concrete by the action of Bacillus pumilus; Assoc Brasileira Engenharia Sanitaria Ambiental; Engenharia Sanitaria E Ambiental; 28; 10-2023; 1-8
1413-4152
1809-4457
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-41522023000100138&tlng=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/s1413-415220220277
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Assoc Brasileira Engenharia Sanitaria Ambiental
publisher.none.fl_str_mv Assoc Brasileira Engenharia Sanitaria Ambiental
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782011685994496
score 12.982451

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