Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide

Autores
Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.
Fil: Goldstein Raij, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Silberstein, Claudia Marcela. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Materia
ADENYLYL CYLASE
COS-7 CELLS
CYCLIC AMP
NITRIC OXIDE
SIGNAL TRANSDUCTION
SODIUM NITROPRUSSIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/152212

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network_name_str CONICET Digital (CONICET)
spelling Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxideGoldstein Raij, JorgeIbarra, Cristina AdrianaSilberstein, Claudia MarcelaADENYLYL CYLASECOS-7 CELLSCYCLIC AMPNITRIC OXIDESIGNAL TRANSDUCTIONSODIUM NITROPRUSSIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.Fil: Goldstein Raij, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaFil: Silberstein, Claudia Marcela. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaAssociação Brasileira de Divulgação Científica2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152212Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela; Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 35; 2; 12-2002; 145-1510100-879X1414-431XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/bjmbr/a/DkM6kWr5mgTkc6wrYGycngH/?lang=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2002000200002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:58:01Zoai:ri.conicet.gov.ar:11336/152212instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:58:01.567CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
title Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
spellingShingle Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
Goldstein Raij, Jorge
ADENYLYL CYLASE
COS-7 CELLS
CYCLIC AMP
NITRIC OXIDE
SIGNAL TRANSDUCTION
SODIUM NITROPRUSSIDE
title_short Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
title_full Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
title_fullStr Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
title_full_unstemmed Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
title_sort Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
dc.creator.none.fl_str_mv Goldstein Raij, Jorge
Ibarra, Cristina Adriana
Silberstein, Claudia Marcela
author Goldstein Raij, Jorge
author_facet Goldstein Raij, Jorge
Ibarra, Cristina Adriana
Silberstein, Claudia Marcela
author_role author
author2 Ibarra, Cristina Adriana
Silberstein, Claudia Marcela
author2_role author
author
dc.subject.none.fl_str_mv ADENYLYL CYLASE
COS-7 CELLS
CYCLIC AMP
NITRIC OXIDE
SIGNAL TRANSDUCTION
SODIUM NITROPRUSSIDE
topic ADENYLYL CYLASE
COS-7 CELLS
CYCLIC AMP
NITRIC OXIDE
SIGNAL TRANSDUCTION
SODIUM NITROPRUSSIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.
Fil: Goldstein Raij, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Silberstein, Claudia Marcela. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
description Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.
publishDate 2002
dc.date.none.fl_str_mv 2002-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152212
Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela; Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 35; 2; 12-2002; 145-151
0100-879X
1414-431X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152212
identifier_str_mv Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela; Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 35; 2; 12-2002; 145-151
0100-879X
1414-431X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/bjmbr/a/DkM6kWr5mgTkc6wrYGycngH/?lang=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2002000200002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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