Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
- Autores
- Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.
Fil: Goldstein Raij, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Silberstein, Claudia Marcela. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina - Materia
-
ADENYLYL CYLASE
COS-7 CELLS
CYCLIC AMP
NITRIC OXIDE
SIGNAL TRANSDUCTION
SODIUM NITROPRUSSIDE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/152212
Ver los metadatos del registro completo
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spelling |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxideGoldstein Raij, JorgeIbarra, Cristina AdrianaSilberstein, Claudia MarcelaADENYLYL CYLASECOS-7 CELLSCYCLIC AMPNITRIC OXIDESIGNAL TRANSDUCTIONSODIUM NITROPRUSSIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.Fil: Goldstein Raij, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaFil: Silberstein, Claudia Marcela. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaAssociação Brasileira de Divulgação Científica2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152212Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela; Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 35; 2; 12-2002; 145-1510100-879X1414-431XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/bjmbr/a/DkM6kWr5mgTkc6wrYGycngH/?lang=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2002000200002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:58:01Zoai:ri.conicet.gov.ar:11336/152212instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:58:01.567CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
title |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
spellingShingle |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide Goldstein Raij, Jorge ADENYLYL CYLASE COS-7 CELLS CYCLIC AMP NITRIC OXIDE SIGNAL TRANSDUCTION SODIUM NITROPRUSSIDE |
title_short |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
title_full |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
title_fullStr |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
title_full_unstemmed |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
title_sort |
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide |
dc.creator.none.fl_str_mv |
Goldstein Raij, Jorge Ibarra, Cristina Adriana Silberstein, Claudia Marcela |
author |
Goldstein Raij, Jorge |
author_facet |
Goldstein Raij, Jorge Ibarra, Cristina Adriana Silberstein, Claudia Marcela |
author_role |
author |
author2 |
Ibarra, Cristina Adriana Silberstein, Claudia Marcela |
author2_role |
author author |
dc.subject.none.fl_str_mv |
ADENYLYL CYLASE COS-7 CELLS CYCLIC AMP NITRIC OXIDE SIGNAL TRANSDUCTION SODIUM NITROPRUSSIDE |
topic |
ADENYLYL CYLASE COS-7 CELLS CYCLIC AMP NITRIC OXIDE SIGNAL TRANSDUCTION SODIUM NITROPRUSSIDE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V. Fil: Goldstein Raij, Jorge. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Silberstein, Claudia Marcela. Universidad de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina |
description |
Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3′,5′-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (∼98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (∼76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/152212 Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela; Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 35; 2; 12-2002; 145-151 0100-879X 1414-431X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/152212 |
identifier_str_mv |
Goldstein Raij, Jorge; Ibarra, Cristina Adriana; Silberstein, Claudia Marcela; Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 35; 2; 12-2002; 145-151 0100-879X 1414-431X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/bjmbr/a/DkM6kWr5mgTkc6wrYGycngH/?lang=en info:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2002000200002 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846782293588312064 |
score |
13.199325 |