Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.

Autores
Wertheimer Hermitte, Eva Victoria; Krapf, Dario; de la Vega Beltran, José L.; Sánchez Cárdenas, Claudia; Navarrete, Felipe; Haddad, Douglas; Escoffier, Jessica; Salicioni, Ana M.; Levin, Lonny R.; Buck, Jochen; Mager, Jesse; Darszon, Alberto; Visconti, Pablo E.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum.
Fil: Wertheimer Hermitte, Eva Victoria. University Of Massachussets; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Krapf, Dario. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: de la Vega Beltran, José L.. Universidad Nacional Autónoma de México; México
Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; México
Fil: Navarrete, Felipe. University Of Massachussets; Estados Unidos
Fil: Haddad, Douglas. University Of Massachussets; Estados Unidos
Fil: Escoffier, Jessica. University Of Massachussets; Estados Unidos
Fil: Salicioni, Ana M.. University Of Massachussets; Estados Unidos
Fil: Levin, Lonny R.. Cornell University; Estados Unidos
Fil: Buck, Jochen. Cornell University; Estados Unidos
Fil: Mager, Jesse. University Of Massachussets; Estados Unidos
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México
Fil: Visconti, Pablo E.. University Of Massachussets; Estados Unidos
Materia
acrosome reaction
adenilate cyclase (adenylyl cyclase)
calcium imaging
cell signaling
cyclic AMP (cAMP)
forskolin
heterotrimeric G proteins
protein kinase A (PKA)
signal transduction
sperm capacitation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13822
Acceder
id CONICETDig_639bf2a109e52731dd1e3e86a79f4d2c
oai_identifier_str oai:ri.conicet.gov.ar:11336/13822
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.Wertheimer Hermitte, Eva VictoriaKrapf, Dariode la Vega Beltran, José L.Sánchez Cárdenas, ClaudiaNavarrete, FelipeHaddad, DouglasEscoffier, JessicaSalicioni, Ana M.Levin, Lonny R.Buck, JochenMager, JesseDarszon, AlbertoVisconti, Pablo E.acrosome reactionadenilate cyclase (adenylyl cyclase)calcium imagingcell signalingcyclic AMP (cAMP)forskolinheterotrimeric G proteinsprotein kinase A (PKA)signal transductionsperm capacitationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum.Fil: Wertheimer Hermitte, Eva Victoria. University Of Massachussets; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Krapf, Dario. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: de la Vega Beltran, José L.. Universidad Nacional Autónoma de México; MéxicoFil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; MéxicoFil: Navarrete, Felipe. University Of Massachussets; Estados UnidosFil: Haddad, Douglas. University Of Massachussets; Estados UnidosFil: Escoffier, Jessica. University Of Massachussets; Estados UnidosFil: Salicioni, Ana M.. University Of Massachussets; Estados UnidosFil: Levin, Lonny R.. Cornell University; Estados UnidosFil: Buck, Jochen. Cornell University; Estados UnidosFil: Mager, Jesse. University Of Massachussets; Estados UnidosFil: Darszon, Alberto. Universidad Nacional Autónoma de México; MéxicoFil: Visconti, Pablo E.. University Of Massachussets; Estados UnidosAmerican Society for Biochemistry and Molecular Biology2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13822Wertheimer Hermitte, Eva Victoria; Krapf, Dario; de la Vega Beltran, José L.; Sánchez Cárdenas, Claudia; Navarrete, Felipe; et al.; Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.; American Society for Biochemistry and Molecular Biology; Journal Of Biological Chemistry (online); 288; 49; 12-2013; 35307-353201083-351Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/early/2013/10/15/jbc.M113.489476info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M113.489476info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:53Zoai:ri.conicet.gov.ar:11336/13822instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:54.191CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
title Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
spellingShingle Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
Wertheimer Hermitte, Eva Victoria
acrosome reaction
adenilate cyclase (adenylyl cyclase)
calcium imaging
cell signaling
cyclic AMP (cAMP)
forskolin
heterotrimeric G proteins
protein kinase A (PKA)
signal transduction
sperm capacitation
title_short Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
title_full Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
title_fullStr Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
title_full_unstemmed Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
title_sort Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
dc.creator.none.fl_str_mv Wertheimer Hermitte, Eva Victoria
Krapf, Dario
de la Vega Beltran, José L.
Sánchez Cárdenas, Claudia
Navarrete, Felipe
Haddad, Douglas
Escoffier, Jessica
Salicioni, Ana M.
Levin, Lonny R.
Buck, Jochen
Mager, Jesse
Darszon, Alberto
Visconti, Pablo E.
author Wertheimer Hermitte, Eva Victoria
author_facet Wertheimer Hermitte, Eva Victoria
Krapf, Dario
de la Vega Beltran, José L.
Sánchez Cárdenas, Claudia
Navarrete, Felipe
Haddad, Douglas
Escoffier, Jessica
Salicioni, Ana M.
Levin, Lonny R.
Buck, Jochen
Mager, Jesse
Darszon, Alberto
Visconti, Pablo E.
author_role author
author2 Krapf, Dario
de la Vega Beltran, José L.
Sánchez Cárdenas, Claudia
Navarrete, Felipe
Haddad, Douglas
Escoffier, Jessica
Salicioni, Ana M.
Levin, Lonny R.
Buck, Jochen
Mager, Jesse
Darszon, Alberto
Visconti, Pablo E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv acrosome reaction
adenilate cyclase (adenylyl cyclase)
calcium imaging
cell signaling
cyclic AMP (cAMP)
forskolin
heterotrimeric G proteins
protein kinase A (PKA)
signal transduction
sperm capacitation
topic acrosome reaction
adenilate cyclase (adenylyl cyclase)
calcium imaging
cell signaling
cyclic AMP (cAMP)
forskolin
heterotrimeric G proteins
protein kinase A (PKA)
signal transduction
sperm capacitation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum.
Fil: Wertheimer Hermitte, Eva Victoria. University Of Massachussets; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Krapf, Dario. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: de la Vega Beltran, José L.. Universidad Nacional Autónoma de México; México
Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; México
Fil: Navarrete, Felipe. University Of Massachussets; Estados Unidos
Fil: Haddad, Douglas. University Of Massachussets; Estados Unidos
Fil: Escoffier, Jessica. University Of Massachussets; Estados Unidos
Fil: Salicioni, Ana M.. University Of Massachussets; Estados Unidos
Fil: Levin, Lonny R.. Cornell University; Estados Unidos
Fil: Buck, Jochen. Cornell University; Estados Unidos
Fil: Mager, Jesse. University Of Massachussets; Estados Unidos
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México
Fil: Visconti, Pablo E.. University Of Massachussets; Estados Unidos
description Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13822
Wertheimer Hermitte, Eva Victoria; Krapf, Dario; de la Vega Beltran, José L.; Sánchez Cárdenas, Claudia; Navarrete, Felipe; et al.; Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.; American Society for Biochemistry and Molecular Biology; Journal Of Biological Chemistry (online); 288; 49; 12-2013; 35307-35320
1083-351X
url http://hdl.handle.net/11336/13822
identifier_str_mv Wertheimer Hermitte, Eva Victoria; Krapf, Dario; de la Vega Beltran, José L.; Sánchez Cárdenas, Claudia; Navarrete, Felipe; et al.; Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.; American Society for Biochemistry and Molecular Biology; Journal Of Biological Chemistry (online); 288; 49; 12-2013; 35307-35320
1083-351X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/early/2013/10/15/jbc.M113.489476
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M113.489476
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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