Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation

Autores
Igata, Manami; Islam, M. Aminul; Tada, Asuka; Takagi, Michihiro; Humayun Kober, AKM; Albarracín, Leonardo Miguel; Aso, Hisashi; Ikeda-Ohtsubo, Wakako; Miyazawa, Kenji; Yoda, Kazutoyo; He, Fang; Takahashi, Hideki; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that EGR1, NOTCH1, NOS2, TNFAIP3, TRAF3IP1, INSR, CXCR4, PPARA, MAPK10, and C3 are the top 10 commonly altered genes of TLRs induced transcriptional modification of pMA. However, the protein-protein interaction network of DEGs identified EPOR, C3, STAR, CCL2, and SAA2 as the major hub genes, which were also exhibited higher centrality estimates in the Gene-Transcription factor interaction network. Our results provide new insights of transcriptome modifications associated with TLRs activation in porcine adipocytes and identified key regulatory genes that could be used as biomarkers for the evaluation of treatments having immunomodularoty and/or metabolic functional beneficial effects in porcine adipocytes.
Fil: Igata, Manami. Tohoku University; Japón
Fil: Islam, M. Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Tada, Asuka. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Humayun Kober, AKM. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University; Bangladesh
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Miyazawa, Kenji. Takanashi Milk Products Co.; Japón
Fil: Yoda, Kazutoyo. Takanashi Milk Products Co.; Japón
Fil: He, Fang. Takanashi Milk Products Co.; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
ADIPOCYTES
IMMUNOMETABOLISM
MICROARRAY
PIG
TLRS (TOLL-LIKE RECEPTORS)
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96619
Acceder
id CONICETDig_95df086632123073f5cf38465e55ecc1
oai_identifier_str oai:ri.conicet.gov.ar:11336/96619
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors ActivationIgata, ManamiIslam, M. AminulTada, AsukaTakagi, MichihiroHumayun Kober, AKMAlbarracín, Leonardo MiguelAso, HisashiIkeda-Ohtsubo, WakakoMiyazawa, KenjiYoda, KazutoyoHe, FangTakahashi, HidekiVillena, Julio CesarKitazawa, HarukiADIPOCYTESIMMUNOMETABOLISMMICROARRAYPIGTLRS (TOLL-LIKE RECEPTORS)TRANSCRIPTOMEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that EGR1, NOTCH1, NOS2, TNFAIP3, TRAF3IP1, INSR, CXCR4, PPARA, MAPK10, and C3 are the top 10 commonly altered genes of TLRs induced transcriptional modification of pMA. However, the protein-protein interaction network of DEGs identified EPOR, C3, STAR, CCL2, and SAA2 as the major hub genes, which were also exhibited higher centrality estimates in the Gene-Transcription factor interaction network. Our results provide new insights of transcriptome modifications associated with TLRs activation in porcine adipocytes and identified key regulatory genes that could be used as biomarkers for the evaluation of treatments having immunomodularoty and/or metabolic functional beneficial effects in porcine adipocytes.Fil: Igata, Manami. Tohoku University; JapónFil: Islam, M. Aminul. Tohoku University; Japón. Bangladesh Agricultural University; BangladeshFil: Tada, Asuka. Tohoku University; JapónFil: Takagi, Michihiro. Tohoku University; JapónFil: Humayun Kober, AKM. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University; BangladeshFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; ArgentinaFil: Aso, Hisashi. Tohoku University; JapónFil: Ikeda-Ohtsubo, Wakako. Tohoku University; JapónFil: Miyazawa, Kenji. Takanashi Milk Products Co.; JapónFil: Yoda, Kazutoyo. Takanashi Milk Products Co.; JapónFil: He, Fang. Takanashi Milk Products Co.; JapónFil: Takahashi, Hideki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónFrontiers Media S.A.2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96619Igata, Manami; Islam, M. Aminul; Tada, Asuka; Takagi, Michihiro; Humayun Kober, AKM; et al.; Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation; Frontiers Media S.A.; Frontiers in Immunology; 10; 5-2019; 1-151664-32241664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2019.01180info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2019.01180/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:49Zoai:ri.conicet.gov.ar:11336/96619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:49.773CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
title Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
spellingShingle Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
Igata, Manami
ADIPOCYTES
IMMUNOMETABOLISM
MICROARRAY
PIG
TLRS (TOLL-LIKE RECEPTORS)
TRANSCRIPTOME
title_short Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
title_full Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
title_fullStr Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
title_full_unstemmed Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
title_sort Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation
dc.creator.none.fl_str_mv Igata, Manami
Islam, M. Aminul
Tada, Asuka
Takagi, Michihiro
Humayun Kober, AKM
Albarracín, Leonardo Miguel
Aso, Hisashi
Ikeda-Ohtsubo, Wakako
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Kitazawa, Haruki
author Igata, Manami
author_facet Igata, Manami
Islam, M. Aminul
Tada, Asuka
Takagi, Michihiro
Humayun Kober, AKM
Albarracín, Leonardo Miguel
Aso, Hisashi
Ikeda-Ohtsubo, Wakako
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Islam, M. Aminul
Tada, Asuka
Takagi, Michihiro
Humayun Kober, AKM
Albarracín, Leonardo Miguel
Aso, Hisashi
Ikeda-Ohtsubo, Wakako
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOCYTES
IMMUNOMETABOLISM
MICROARRAY
PIG
TLRS (TOLL-LIKE RECEPTORS)
TRANSCRIPTOME
topic ADIPOCYTES
IMMUNOMETABOLISM
MICROARRAY
PIG
TLRS (TOLL-LIKE RECEPTORS)
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that EGR1, NOTCH1, NOS2, TNFAIP3, TRAF3IP1, INSR, CXCR4, PPARA, MAPK10, and C3 are the top 10 commonly altered genes of TLRs induced transcriptional modification of pMA. However, the protein-protein interaction network of DEGs identified EPOR, C3, STAR, CCL2, and SAA2 as the major hub genes, which were also exhibited higher centrality estimates in the Gene-Transcription factor interaction network. Our results provide new insights of transcriptome modifications associated with TLRs activation in porcine adipocytes and identified key regulatory genes that could be used as biomarkers for the evaluation of treatments having immunomodularoty and/or metabolic functional beneficial effects in porcine adipocytes.
Fil: Igata, Manami. Tohoku University; Japón
Fil: Islam, M. Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Tada, Asuka. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Humayun Kober, AKM. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University; Bangladesh
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Miyazawa, Kenji. Takanashi Milk Products Co.; Japón
Fil: Yoda, Kazutoyo. Takanashi Milk Products Co.; Japón
Fil: He, Fang. Takanashi Milk Products Co.; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that EGR1, NOTCH1, NOS2, TNFAIP3, TRAF3IP1, INSR, CXCR4, PPARA, MAPK10, and C3 are the top 10 commonly altered genes of TLRs induced transcriptional modification of pMA. However, the protein-protein interaction network of DEGs identified EPOR, C3, STAR, CCL2, and SAA2 as the major hub genes, which were also exhibited higher centrality estimates in the Gene-Transcription factor interaction network. Our results provide new insights of transcriptome modifications associated with TLRs activation in porcine adipocytes and identified key regulatory genes that could be used as biomarkers for the evaluation of treatments having immunomodularoty and/or metabolic functional beneficial effects in porcine adipocytes.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96619
Igata, Manami; Islam, M. Aminul; Tada, Asuka; Takagi, Michihiro; Humayun Kober, AKM; et al.; Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation; Frontiers Media S.A.; Frontiers in Immunology; 10; 5-2019; 1-15
1664-3224
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96619
identifier_str_mv Igata, Manami; Islam, M. Aminul; Tada, Asuka; Takagi, Michihiro; Humayun Kober, AKM; et al.; Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation; Frontiers Media S.A.; Frontiers in Immunology; 10; 5-2019; 1-15
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2019.01180
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2019.01180/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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