Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil

Autores
Loto, Flavia del Valle; Romero, Cintia Mariana; Carrizo, Alfonso Emanuel; Baigori, Mario Domingo; Pera, Licia Maria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel esterase producer strain named Bacillus sp. A60 was isolated from a soil sample contaminated with hydrocarbons. It was found to belong to Bacillus subtilis species through morphological, biochemical and 16S rRNA gene sequence analyses. This strain which can tolerate 15% (w/v) NaCl and growth at 55°C, produced an interesting esterase activity in Luria-Bertani medium. Two different molecular weight esterase activities were detected in zymographic assays. Culture supernatant and whole cells showed specific hydrolytic activities of 2.67 ± 0.11 U/mg of protein and 7.07 ± 0.09 U/mg of dry weight, respectively. Concerning ethyl acetate production, conversions of 88.00 ± 0 and 55.58 ± 0.78% were obtained with culture supernatant entrapped in polyacrylamine gel and whole cells, respectively. In addition, the effect of different concentration of LB medium components on both growth and extracellular esterase hydrolytic activity was also discussed.
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
BACILLUS SP. A60
ESTERASES
HYDROLYTIC ACTIVITY
SYNTHETIC ACTIVITY
ETHYL ACETATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1569
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1569
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soilLoto, Flavia del ValleRomero, Cintia MarianaCarrizo, Alfonso EmanuelBaigori, Mario DomingoPera, Licia MariaBACILLUS SP. A60ESTERASESHYDROLYTIC ACTIVITYSYNTHETIC ACTIVITYETHYL ACETATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A novel esterase producer strain named Bacillus sp. A60 was isolated from a soil sample contaminated with hydrocarbons. It was found to belong to Bacillus subtilis species through morphological, biochemical and 16S rRNA gene sequence analyses. This strain which can tolerate 15% (w/v) NaCl and growth at 55°C, produced an interesting esterase activity in Luria-Bertani medium. Two different molecular weight esterase activities were detected in zymographic assays. Culture supernatant and whole cells showed specific hydrolytic activities of 2.67 ± 0.11 U/mg of protein and 7.07 ± 0.09 U/mg of dry weight, respectively. Concerning ethyl acetate production, conversions of 88.00 ± 0 and 55.58 ± 0.78% were obtained with culture supernatant entrapped in polyacrylamine gel and whole cells, respectively. In addition, the effect of different concentration of LB medium components on both growth and extracellular esterase hydrolytic activity was also discussed.Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaAcademic Journals2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1569Loto, Flavia del Valle; Romero, Cintia Mariana; Carrizo, Alfonso Emanuel; Baigori, Mario Domingo; Pera, Licia Maria; Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil; Academic Journals; African Journal of Biotechnology; 12; 11-2013; 6625-66311684-5315enginfo:eu-repo/semantics/altIdentifier/doi/10.5897/AJB2013.12123info:eu-repo/semantics/altIdentifier/url/http://www.academicjournals.org/article/article1385139810_Loto%20et%20al.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:15Zoai:ri.conicet.gov.ar:11336/1569instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:15.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
title Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
spellingShingle Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
Loto, Flavia del Valle
BACILLUS SP. A60
ESTERASES
HYDROLYTIC ACTIVITY
SYNTHETIC ACTIVITY
ETHYL ACETATE
title_short Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
title_full Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
title_fullStr Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
title_full_unstemmed Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
title_sort Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
dc.creator.none.fl_str_mv Loto, Flavia del Valle
Romero, Cintia Mariana
Carrizo, Alfonso Emanuel
Baigori, Mario Domingo
Pera, Licia Maria
author Loto, Flavia del Valle
author_facet Loto, Flavia del Valle
Romero, Cintia Mariana
Carrizo, Alfonso Emanuel
Baigori, Mario Domingo
Pera, Licia Maria
author_role author
author2 Romero, Cintia Mariana
Carrizo, Alfonso Emanuel
Baigori, Mario Domingo
Pera, Licia Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv BACILLUS SP. A60
ESTERASES
HYDROLYTIC ACTIVITY
SYNTHETIC ACTIVITY
ETHYL ACETATE
topic BACILLUS SP. A60
ESTERASES
HYDROLYTIC ACTIVITY
SYNTHETIC ACTIVITY
ETHYL ACETATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A novel esterase producer strain named Bacillus sp. A60 was isolated from a soil sample contaminated with hydrocarbons. It was found to belong to Bacillus subtilis species through morphological, biochemical and 16S rRNA gene sequence analyses. This strain which can tolerate 15% (w/v) NaCl and growth at 55°C, produced an interesting esterase activity in Luria-Bertani medium. Two different molecular weight esterase activities were detected in zymographic assays. Culture supernatant and whole cells showed specific hydrolytic activities of 2.67 ± 0.11 U/mg of protein and 7.07 ± 0.09 U/mg of dry weight, respectively. Concerning ethyl acetate production, conversions of 88.00 ± 0 and 55.58 ± 0.78% were obtained with culture supernatant entrapped in polyacrylamine gel and whole cells, respectively. In addition, the effect of different concentration of LB medium components on both growth and extracellular esterase hydrolytic activity was also discussed.
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description A novel esterase producer strain named Bacillus sp. A60 was isolated from a soil sample contaminated with hydrocarbons. It was found to belong to Bacillus subtilis species through morphological, biochemical and 16S rRNA gene sequence analyses. This strain which can tolerate 15% (w/v) NaCl and growth at 55°C, produced an interesting esterase activity in Luria-Bertani medium. Two different molecular weight esterase activities were detected in zymographic assays. Culture supernatant and whole cells showed specific hydrolytic activities of 2.67 ± 0.11 U/mg of protein and 7.07 ± 0.09 U/mg of dry weight, respectively. Concerning ethyl acetate production, conversions of 88.00 ± 0 and 55.58 ± 0.78% were obtained with culture supernatant entrapped in polyacrylamine gel and whole cells, respectively. In addition, the effect of different concentration of LB medium components on both growth and extracellular esterase hydrolytic activity was also discussed.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1569
Loto, Flavia del Valle; Romero, Cintia Mariana; Carrizo, Alfonso Emanuel; Baigori, Mario Domingo; Pera, Licia Maria; Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil; Academic Journals; African Journal of Biotechnology; 12; 11-2013; 6625-6631
1684-5315
url http://hdl.handle.net/11336/1569
identifier_str_mv Loto, Flavia del Valle; Romero, Cintia Mariana; Carrizo, Alfonso Emanuel; Baigori, Mario Domingo; Pera, Licia Maria; Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil; Academic Journals; African Journal of Biotechnology; 12; 11-2013; 6625-6631
1684-5315
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5897/AJB2013.12123
info:eu-repo/semantics/altIdentifier/url/http://www.academicjournals.org/article/article1385139810_Loto%20et%20al.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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