Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase

Autores
Torres, Sebastián; Baigori, Mario Domingo; Swathy, S. L.; Ashok Pandey; Castro, Guillermo Raul
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
New carboxylesterase from organic-solvent-tolerant Bacillus licheniformis S-86 strain was characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60–65 °C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1 h at 50 °C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0–11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1% ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28 °C) and with a very low amount of enzyme (4.6 × 10−5 mg ml−1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes.
Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Swathy, S. L.. Indian Institute of Technology; India
Fil: Ashok Pandey. Indian Institute of Technology; India
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Tufts University; Estados Unidos
Materia
Esterases
Bacillus Licheniformis Esterase
Transesterification
Isoamyl Acetate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43396
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43396
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esteraseTorres, SebastiánBaigori, Mario DomingoSwathy, S. L.Ashok PandeyCastro, Guillermo RaulEsterasesBacillus Licheniformis EsteraseTransesterificationIsoamyl Acetatehttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2New carboxylesterase from organic-solvent-tolerant Bacillus licheniformis S-86 strain was characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60–65 °C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1 h at 50 °C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0–11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1% ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28 °C) and with a very low amount of enzyme (4.6 × 10−5 mg ml−1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes.Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Swathy, S. L.. Indian Institute of Technology; IndiaFil: Ashok Pandey. Indian Institute of Technology; IndiaFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Tufts University; Estados UnidosElsevier Science2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43396Torres, Sebastián; Baigori, Mario Domingo; Swathy, S. L.; Ashok Pandey; Castro, Guillermo Raul; Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase; Elsevier Science; Food Research International; 42; 4; 12-2009; 454-4600963-9969CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0963996908002469info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2008.12.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:32:53Zoai:ri.conicet.gov.ar:11336/43396instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:32:53.3CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
title Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
spellingShingle Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
Torres, Sebastián
Esterases
Bacillus Licheniformis Esterase
Transesterification
Isoamyl Acetate
title_short Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
title_full Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
title_fullStr Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
title_full_unstemmed Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
title_sort Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase
dc.creator.none.fl_str_mv Torres, Sebastián
Baigori, Mario Domingo
Swathy, S. L.
Ashok Pandey
Castro, Guillermo Raul
author Torres, Sebastián
author_facet Torres, Sebastián
Baigori, Mario Domingo
Swathy, S. L.
Ashok Pandey
Castro, Guillermo Raul
author_role author
author2 Baigori, Mario Domingo
Swathy, S. L.
Ashok Pandey
Castro, Guillermo Raul
author2_role author
author
author
author
dc.subject.none.fl_str_mv Esterases
Bacillus Licheniformis Esterase
Transesterification
Isoamyl Acetate
topic Esterases
Bacillus Licheniformis Esterase
Transesterification
Isoamyl Acetate
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv New carboxylesterase from organic-solvent-tolerant Bacillus licheniformis S-86 strain was characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60–65 °C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1 h at 50 °C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0–11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1% ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28 °C) and with a very low amount of enzyme (4.6 × 10−5 mg ml−1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes.
Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Swathy, S. L.. Indian Institute of Technology; India
Fil: Ashok Pandey. Indian Institute of Technology; India
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Tufts University; Estados Unidos
description New carboxylesterase from organic-solvent-tolerant Bacillus licheniformis S-86 strain was characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60–65 °C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1 h at 50 °C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0–11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1% ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28 °C) and with a very low amount of enzyme (4.6 × 10−5 mg ml−1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43396
Torres, Sebastián; Baigori, Mario Domingo; Swathy, S. L.; Ashok Pandey; Castro, Guillermo Raul; Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase; Elsevier Science; Food Research International; 42; 4; 12-2009; 454-460
0963-9969
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43396
identifier_str_mv Torres, Sebastián; Baigori, Mario Domingo; Swathy, S. L.; Ashok Pandey; Castro, Guillermo Raul; Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase; Elsevier Science; Food Research International; 42; 4; 12-2009; 454-460
0963-9969
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0963996908002469
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2008.12.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606689813626880
score 13.000565

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