Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms

Autores
Longhi, Silvia Andrea; Brandariz, Silvia B.; Lafon, Sonia Olga; Niborski, Leticia Laura; Luquetti, Alejandro O.; Schijman, Alejandro Gabriel; Levin, Mariano Jorge; Gomez, Karina Andrea
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease.
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; Brasil
Fil: Brandariz, Silvia B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; Brasil
Fil: Lafon, Sonia Olga. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Niborski, Leticia Laura. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Luquetti, Alejandro O.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; Brasil
Fil: Levin, Mariano Jorge. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gomez, Karina Andrea. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Trypanosoma Cruzi
Diagnosis
Elisa Test
Chagas Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79363
Acceder
id CONICETDig_95a8d92c0bf16da905be29283756eb76
oai_identifier_str oai:ri.conicet.gov.ar:11336/79363
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical formsLonghi, Silvia AndreaBrandariz, Silvia B.Lafon, Sonia OlgaNiborski, Leticia LauraLuquetti, Alejandro O.Schijman, Alejandro GabrielLevin, Mariano JorgeGomez, Karina AndreaTrypanosoma CruziDiagnosisElisa TestChagas Diseasehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease.Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; BrasilFil: Brandariz, Silvia B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; BrasilFil: Lafon, Sonia Olga. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Niborski, Leticia Laura. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Luquetti, Alejandro O.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; BrasilFil: Levin, Mariano Jorge. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Gomez, Karina Andrea. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaAmerican Society of Tropical Medicine and Hygiene2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79363Longhi, Silvia Andrea; Brandariz, Silvia B.; Lafon, Sonia Olga; Niborski, Leticia Laura; Luquetti, Alejandro O.; et al.; Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 87; 2; 8-2012; 267-2710002-9637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.2012.11-0533info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2012.11-0533info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414563/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:52:28Zoai:ri.conicet.gov.ar:11336/79363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:52:28.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
title Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
spellingShingle Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
Longhi, Silvia Andrea
Trypanosoma Cruzi
Diagnosis
Elisa Test
Chagas Disease
title_short Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
title_full Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
title_fullStr Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
title_full_unstemmed Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
title_sort Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms
dc.creator.none.fl_str_mv Longhi, Silvia Andrea
Brandariz, Silvia B.
Lafon, Sonia Olga
Niborski, Leticia Laura
Luquetti, Alejandro O.
Schijman, Alejandro Gabriel
Levin, Mariano Jorge
Gomez, Karina Andrea
author Longhi, Silvia Andrea
author_facet Longhi, Silvia Andrea
Brandariz, Silvia B.
Lafon, Sonia Olga
Niborski, Leticia Laura
Luquetti, Alejandro O.
Schijman, Alejandro Gabriel
Levin, Mariano Jorge
Gomez, Karina Andrea
author_role author
author2 Brandariz, Silvia B.
Lafon, Sonia Olga
Niborski, Leticia Laura
Luquetti, Alejandro O.
Schijman, Alejandro Gabriel
Levin, Mariano Jorge
Gomez, Karina Andrea
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma Cruzi
Diagnosis
Elisa Test
Chagas Disease
topic Trypanosoma Cruzi
Diagnosis
Elisa Test
Chagas Disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease.
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; Brasil
Fil: Brandariz, Silvia B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; Brasil
Fil: Lafon, Sonia Olga. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Niborski, Leticia Laura. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Luquetti, Alejandro O.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidade Federal de Goiás; Brasil
Fil: Levin, Mariano Jorge. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gomez, Karina Andrea. Universidade Federal de Goiás; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0b as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79363
Longhi, Silvia Andrea; Brandariz, Silvia B.; Lafon, Sonia Olga; Niborski, Leticia Laura; Luquetti, Alejandro O.; et al.; Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 87; 2; 8-2012; 267-271
0002-9637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79363
identifier_str_mv Longhi, Silvia Andrea; Brandariz, Silvia B.; Lafon, Sonia Olga; Niborski, Leticia Laura; Luquetti, Alejandro O.; et al.; Evaluation of in-house ELISA using Trypanosoma cruzi lysate and recombinant antigens for diagnosis of chagas disease and discrimination of its clinical forms; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 87; 2; 8-2012; 267-271
0002-9637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.2012.11-0533
info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2012.11-0533
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414563/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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