The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer

Autores
Roulet, Maria Emilia; Ceriotti, Luis Federico; Sánchez Puerta, María Virginia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Plant vascular connections established in a host-parasite relationship allow the passage of water, nutrients, and nucleic acids. This intimate contact promotes the exchange of genetic information between unrelated species, a process named Horizontal Gene Transfer (HGT). Plant mitochondrial genomes (mtDNAs) are frequently impacted by HGT. We deciphered the mtDNA of the holoparasitic angiosperm Lophophytum leandri (Balanophoraceae). This plant grows in Argentina parasitizing roots of the mimosoid legume Parapiptadenia rigida (Fabaceae). The L. leandri mtDNA is 806,114 bp long and has a multichromosomal structure consisting of 50 circular chromosomes (5-72 kb in length). This multipartite genome was recently described in the relatives L. mirabile and Ombrophytum subterraneum. Similarity searches revealed 46.37% of shared regions between the mtDNA of L. mirabile and L. leandri. The L. leandri mtDNA carries 3 ribosomal RNA, 6 transfer RNA, and 35 protein genes representing 7.25% of the genome and are distributed across 23 chromosomes. The remaining 27 chromosomes are devoid of any identifiable coding region. Comprehensive phylogenetic analyses showed that L. leandri harbors 5 foreign and 15 chimeric mitochondrial protein genes as a result of HGT from its legume hosts. To unveil the overall impact of the HGT in the L. leandri mtDNA, we performed BLAST searches against angiosperm mtDNAs. The L. leandri mtDNA presents 48.3% foreign sequences acquired from legumes and 31.9% native DNA, while 19.6% remains undetermined, as it is not similar to any other angiosperm mtDNA. The availability of the L. leandri mtDNA permitted a genus-wide evolutionary assessment. The analyses uncovered the presence of 19 chimeric genes in L. mirabile, representing almost half (43.18%) of the protein genes. Besides, we found evidence for ancient HGT events, in which foreign mitochondrial genes or regions were acquired before the divergence of L. mirabile and L. leandri.
Fil: Roulet, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
II Virtual Meeting of Systematics, Biogeography, and Evolution: The Research of Biodiversity and the Diversity of Researchers
.
Estados Unidos
SBE Meeting 2021’s Organizing Committee
Materia
BALANOPHORACEAE
MITOCHONDRIAL
GENOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192761
Acceder
id CONICETDig_950bc3ec4857066ed0b464b72ee18b4f
oai_identifier_str oai:ri.conicet.gov.ar:11336/192761
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transferRoulet, Maria EmiliaCeriotti, Luis FedericoSánchez Puerta, María VirginiaBALANOPHORACEAEMITOCHONDRIALGENOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant vascular connections established in a host-parasite relationship allow the passage of water, nutrients, and nucleic acids. This intimate contact promotes the exchange of genetic information between unrelated species, a process named Horizontal Gene Transfer (HGT). Plant mitochondrial genomes (mtDNAs) are frequently impacted by HGT. We deciphered the mtDNA of the holoparasitic angiosperm Lophophytum leandri (Balanophoraceae). This plant grows in Argentina parasitizing roots of the mimosoid legume Parapiptadenia rigida (Fabaceae). The L. leandri mtDNA is 806,114 bp long and has a multichromosomal structure consisting of 50 circular chromosomes (5-72 kb in length). This multipartite genome was recently described in the relatives L. mirabile and Ombrophytum subterraneum. Similarity searches revealed 46.37% of shared regions between the mtDNA of L. mirabile and L. leandri. The L. leandri mtDNA carries 3 ribosomal RNA, 6 transfer RNA, and 35 protein genes representing 7.25% of the genome and are distributed across 23 chromosomes. The remaining 27 chromosomes are devoid of any identifiable coding region. Comprehensive phylogenetic analyses showed that L. leandri harbors 5 foreign and 15 chimeric mitochondrial protein genes as a result of HGT from its legume hosts. To unveil the overall impact of the HGT in the L. leandri mtDNA, we performed BLAST searches against angiosperm mtDNAs. The L. leandri mtDNA presents 48.3% foreign sequences acquired from legumes and 31.9% native DNA, while 19.6% remains undetermined, as it is not similar to any other angiosperm mtDNA. The availability of the L. leandri mtDNA permitted a genus-wide evolutionary assessment. The analyses uncovered the presence of 19 chimeric genes in L. mirabile, representing almost half (43.18%) of the protein genes. Besides, we found evidence for ancient HGT events, in which foreign mitochondrial genes or regions were acquired before the divergence of L. mirabile and L. leandri.Fil: Roulet, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaII Virtual Meeting of Systematics, Biogeography, and Evolution: The Research of Biodiversity and the Diversity of Researchers.Estados UnidosSBE Meeting 2021’s Organizing CommitteeSystematics, Biogeography, and Evolution meeting 2021's organizing committe2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192761The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer; II Virtual Meeting of Systematics, Biogeography, and Evolution: The Research of Biodiversity and the Diversity of Researchers; .; Estados Unidos; 2021; 103-104CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://zenodo.org/record/5109989#.YRxqWdNKhTYinfo:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.5109989Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:11Zoai:ri.conicet.gov.ar:11336/192761instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:11.889CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
title The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
spellingShingle The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
Roulet, Maria Emilia
BALANOPHORACEAE
MITOCHONDRIAL
GENOME
title_short The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
title_full The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
title_fullStr The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
title_full_unstemmed The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
title_sort The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer
dc.creator.none.fl_str_mv Roulet, Maria Emilia
Ceriotti, Luis Federico
Sánchez Puerta, María Virginia
author Roulet, Maria Emilia
author_facet Roulet, Maria Emilia
Ceriotti, Luis Federico
Sánchez Puerta, María Virginia
author_role author
author2 Ceriotti, Luis Federico
Sánchez Puerta, María Virginia
author2_role author
author
dc.subject.none.fl_str_mv BALANOPHORACEAE
MITOCHONDRIAL
GENOME
topic BALANOPHORACEAE
MITOCHONDRIAL
GENOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant vascular connections established in a host-parasite relationship allow the passage of water, nutrients, and nucleic acids. This intimate contact promotes the exchange of genetic information between unrelated species, a process named Horizontal Gene Transfer (HGT). Plant mitochondrial genomes (mtDNAs) are frequently impacted by HGT. We deciphered the mtDNA of the holoparasitic angiosperm Lophophytum leandri (Balanophoraceae). This plant grows in Argentina parasitizing roots of the mimosoid legume Parapiptadenia rigida (Fabaceae). The L. leandri mtDNA is 806,114 bp long and has a multichromosomal structure consisting of 50 circular chromosomes (5-72 kb in length). This multipartite genome was recently described in the relatives L. mirabile and Ombrophytum subterraneum. Similarity searches revealed 46.37% of shared regions between the mtDNA of L. mirabile and L. leandri. The L. leandri mtDNA carries 3 ribosomal RNA, 6 transfer RNA, and 35 protein genes representing 7.25% of the genome and are distributed across 23 chromosomes. The remaining 27 chromosomes are devoid of any identifiable coding region. Comprehensive phylogenetic analyses showed that L. leandri harbors 5 foreign and 15 chimeric mitochondrial protein genes as a result of HGT from its legume hosts. To unveil the overall impact of the HGT in the L. leandri mtDNA, we performed BLAST searches against angiosperm mtDNAs. The L. leandri mtDNA presents 48.3% foreign sequences acquired from legumes and 31.9% native DNA, while 19.6% remains undetermined, as it is not similar to any other angiosperm mtDNA. The availability of the L. leandri mtDNA permitted a genus-wide evolutionary assessment. The analyses uncovered the presence of 19 chimeric genes in L. mirabile, representing almost half (43.18%) of the protein genes. Besides, we found evidence for ancient HGT events, in which foreign mitochondrial genes or regions were acquired before the divergence of L. mirabile and L. leandri.
Fil: Roulet, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
II Virtual Meeting of Systematics, Biogeography, and Evolution: The Research of Biodiversity and the Diversity of Researchers
.
Estados Unidos
SBE Meeting 2021’s Organizing Committee
description Plant vascular connections established in a host-parasite relationship allow the passage of water, nutrients, and nucleic acids. This intimate contact promotes the exchange of genetic information between unrelated species, a process named Horizontal Gene Transfer (HGT). Plant mitochondrial genomes (mtDNAs) are frequently impacted by HGT. We deciphered the mtDNA of the holoparasitic angiosperm Lophophytum leandri (Balanophoraceae). This plant grows in Argentina parasitizing roots of the mimosoid legume Parapiptadenia rigida (Fabaceae). The L. leandri mtDNA is 806,114 bp long and has a multichromosomal structure consisting of 50 circular chromosomes (5-72 kb in length). This multipartite genome was recently described in the relatives L. mirabile and Ombrophytum subterraneum. Similarity searches revealed 46.37% of shared regions between the mtDNA of L. mirabile and L. leandri. The L. leandri mtDNA carries 3 ribosomal RNA, 6 transfer RNA, and 35 protein genes representing 7.25% of the genome and are distributed across 23 chromosomes. The remaining 27 chromosomes are devoid of any identifiable coding region. Comprehensive phylogenetic analyses showed that L. leandri harbors 5 foreign and 15 chimeric mitochondrial protein genes as a result of HGT from its legume hosts. To unveil the overall impact of the HGT in the L. leandri mtDNA, we performed BLAST searches against angiosperm mtDNAs. The L. leandri mtDNA presents 48.3% foreign sequences acquired from legumes and 31.9% native DNA, while 19.6% remains undetermined, as it is not similar to any other angiosperm mtDNA. The availability of the L. leandri mtDNA permitted a genus-wide evolutionary assessment. The analyses uncovered the presence of 19 chimeric genes in L. mirabile, representing almost half (43.18%) of the protein genes. Besides, we found evidence for ancient HGT events, in which foreign mitochondrial genes or regions were acquired before the divergence of L. mirabile and L. leandri.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192761
The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer; II Virtual Meeting of Systematics, Biogeography, and Evolution: The Research of Biodiversity and the Diversity of Researchers; .; Estados Unidos; 2021; 103-104
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192761
identifier_str_mv The mitochondrial genome of holoparasites of the family Balanophoraceae has been severely impacted by horizontal gene transfer; II Virtual Meeting of Systematics, Biogeography, and Evolution: The Research of Biodiversity and the Diversity of Researchers; .; Estados Unidos; 2021; 103-104
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://zenodo.org/record/5109989#.YRxqWdNKhTY
info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.5109989
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Systematics, Biogeography, and Evolution meeting 2021's organizing committe
publisher.none.fl_str_mv Systematics, Biogeography, and Evolution meeting 2021's organizing committe
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980316554199040
score 12.993085

Publicaciones similares