Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant

Autores
Garcia, Laura Evangelina; Edera, Alejandro; Palmer, Jeffrey D.; Sato, Hector Arnaldo; Sánchez Puerta, María Virginia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although horizontal gene transfer (HGT) is common in angiosperm mitochondrial DNAs (mtDNAs), few cases of functional foreign genes have been identified. The one outstanding candidate for large-scale functional HGT is the holoparasite Lophophytum mirabile, whose mtDNA has lost most native genes but contains intact foreign homologs acquired from legume host plants. To investigate the extent to which this situation results from functional replacement of native by foreign genes, functional mitochondrial gene transfer to the nucleus, and/or loss of mitochondrial biochemical function in the context of extreme parasitism, we examined the Lophophytum mitochondrial and nuclear transcriptomes by deep paired-end RNA sequencing. Most foreign mitochondrial genes in Lophophytum are highly transcribed, accurately spliced, and efficiently RNA edited. By contrast, we found no evidence for functional gene transfer to the nucleus or loss of mitochondrial functions in Lophophytum. Many functional replacements occurred via the physical replacement of native genes by foreign genes. Some of these events probably occurred as the final act of HGT itself. Lophophytum mtDNA has experienced an unprecedented level of functional replacement of native genes by foreign copies. This raises important questions concerning population-genetic and molecular regimes that underlie such a high level of foreign gene takeover.
Fil: Garcia, Laura Evangelina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Edera, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Palmer, Jeffrey D.. Indiana University; Estados Unidos
Fil: Sato, Hector Arnaldo. Universidad Nacional de Jujuy; Argentina
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
BALANOPHORACEAE
HOLOPARASITE
LOPHOPHYTUM
MITOCHONDRIAL DNA
RNA EDITING
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128704
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128704
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plantGarcia, Laura EvangelinaEdera, AlejandroPalmer, Jeffrey D.Sato, Hector ArnaldoSánchez Puerta, María VirginiaBALANOPHORACEAEHOLOPARASITELOPHOPHYTUMMITOCHONDRIAL DNARNA EDITINGTRANSCRIPTOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although horizontal gene transfer (HGT) is common in angiosperm mitochondrial DNAs (mtDNAs), few cases of functional foreign genes have been identified. The one outstanding candidate for large-scale functional HGT is the holoparasite Lophophytum mirabile, whose mtDNA has lost most native genes but contains intact foreign homologs acquired from legume host plants. To investigate the extent to which this situation results from functional replacement of native by foreign genes, functional mitochondrial gene transfer to the nucleus, and/or loss of mitochondrial biochemical function in the context of extreme parasitism, we examined the Lophophytum mitochondrial and nuclear transcriptomes by deep paired-end RNA sequencing. Most foreign mitochondrial genes in Lophophytum are highly transcribed, accurately spliced, and efficiently RNA edited. By contrast, we found no evidence for functional gene transfer to the nucleus or loss of mitochondrial functions in Lophophytum. Many functional replacements occurred via the physical replacement of native genes by foreign genes. Some of these events probably occurred as the final act of HGT itself. Lophophytum mtDNA has experienced an unprecedented level of functional replacement of native genes by foreign copies. This raises important questions concerning population-genetic and molecular regimes that underlie such a high level of foreign gene takeover.Fil: Garcia, Laura Evangelina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Edera, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Palmer, Jeffrey D.. Indiana University; Estados UnidosFil: Sato, Hector Arnaldo. Universidad Nacional de Jujuy; ArgentinaFil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaWiley Blackwell Publishing, Inc2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128704Garcia, Laura Evangelina; Edera, Alejandro; Palmer, Jeffrey D.; Sato, Hector Arnaldo; Sánchez Puerta, María Virginia; Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 3; 2-2021; 1701-17140028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/nph.16926info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16926info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.16926info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:03Zoai:ri.conicet.gov.ar:11336/128704instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:03.624CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
title Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
spellingShingle Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
Garcia, Laura Evangelina
BALANOPHORACEAE
HOLOPARASITE
LOPHOPHYTUM
MITOCHONDRIAL DNA
RNA EDITING
TRANSCRIPTOME
title_short Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
title_full Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
title_fullStr Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
title_full_unstemmed Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
title_sort Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant
dc.creator.none.fl_str_mv Garcia, Laura Evangelina
Edera, Alejandro
Palmer, Jeffrey D.
Sato, Hector Arnaldo
Sánchez Puerta, María Virginia
author Garcia, Laura Evangelina
author_facet Garcia, Laura Evangelina
Edera, Alejandro
Palmer, Jeffrey D.
Sato, Hector Arnaldo
Sánchez Puerta, María Virginia
author_role author
author2 Edera, Alejandro
Palmer, Jeffrey D.
Sato, Hector Arnaldo
Sánchez Puerta, María Virginia
author2_role author
author
author
author
dc.subject.none.fl_str_mv BALANOPHORACEAE
HOLOPARASITE
LOPHOPHYTUM
MITOCHONDRIAL DNA
RNA EDITING
TRANSCRIPTOME
topic BALANOPHORACEAE
HOLOPARASITE
LOPHOPHYTUM
MITOCHONDRIAL DNA
RNA EDITING
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although horizontal gene transfer (HGT) is common in angiosperm mitochondrial DNAs (mtDNAs), few cases of functional foreign genes have been identified. The one outstanding candidate for large-scale functional HGT is the holoparasite Lophophytum mirabile, whose mtDNA has lost most native genes but contains intact foreign homologs acquired from legume host plants. To investigate the extent to which this situation results from functional replacement of native by foreign genes, functional mitochondrial gene transfer to the nucleus, and/or loss of mitochondrial biochemical function in the context of extreme parasitism, we examined the Lophophytum mitochondrial and nuclear transcriptomes by deep paired-end RNA sequencing. Most foreign mitochondrial genes in Lophophytum are highly transcribed, accurately spliced, and efficiently RNA edited. By contrast, we found no evidence for functional gene transfer to the nucleus or loss of mitochondrial functions in Lophophytum. Many functional replacements occurred via the physical replacement of native genes by foreign genes. Some of these events probably occurred as the final act of HGT itself. Lophophytum mtDNA has experienced an unprecedented level of functional replacement of native genes by foreign copies. This raises important questions concerning population-genetic and molecular regimes that underlie such a high level of foreign gene takeover.
Fil: Garcia, Laura Evangelina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Edera, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Palmer, Jeffrey D.. Indiana University; Estados Unidos
Fil: Sato, Hector Arnaldo. Universidad Nacional de Jujuy; Argentina
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Although horizontal gene transfer (HGT) is common in angiosperm mitochondrial DNAs (mtDNAs), few cases of functional foreign genes have been identified. The one outstanding candidate for large-scale functional HGT is the holoparasite Lophophytum mirabile, whose mtDNA has lost most native genes but contains intact foreign homologs acquired from legume host plants. To investigate the extent to which this situation results from functional replacement of native by foreign genes, functional mitochondrial gene transfer to the nucleus, and/or loss of mitochondrial biochemical function in the context of extreme parasitism, we examined the Lophophytum mitochondrial and nuclear transcriptomes by deep paired-end RNA sequencing. Most foreign mitochondrial genes in Lophophytum are highly transcribed, accurately spliced, and efficiently RNA edited. By contrast, we found no evidence for functional gene transfer to the nucleus or loss of mitochondrial functions in Lophophytum. Many functional replacements occurred via the physical replacement of native genes by foreign genes. Some of these events probably occurred as the final act of HGT itself. Lophophytum mtDNA has experienced an unprecedented level of functional replacement of native genes by foreign copies. This raises important questions concerning population-genetic and molecular regimes that underlie such a high level of foreign gene takeover.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128704
Garcia, Laura Evangelina; Edera, Alejandro; Palmer, Jeffrey D.; Sato, Hector Arnaldo; Sánchez Puerta, María Virginia; Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 3; 2-2021; 1701-1714
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128704
identifier_str_mv Garcia, Laura Evangelina; Edera, Alejandro; Palmer, Jeffrey D.; Sato, Hector Arnaldo; Sánchez Puerta, María Virginia; Horizontal gene transfers dominate the functional mitochondrial gene space of a holoparasitic plant; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 3; 2-2021; 1701-1714
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1111/nph.16926
info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16926
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.16926
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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