Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage

Autores
Barrios, Melina Elizabeth; Blanco Fernandez, Maria Dolores; Cammarata, Robertina Viviana; Torres, Carolina; Mbayed, Viviana Andrea
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants. The viable F-RNAPh count showed correlation with viable thermotolerant coliforms but also with human polyomaviruses (HPyV) quantified by a new molecular method. In domestic effluents, F-RNAPh and HPyV indicators significantly correlated with a human viral pathogen, norovirus, while the bacterial indicator did not, being then better predictors of the behavior of enteric pathogenic viruses. In addition, we assessed human, bovine and fowl microbial source tracking markers, based on the molecular detections of human polyomavirus, bovine polyomavirus, and fowl adenovirus, respectively. The techniques implemented extend the range of viruses detected, since they target different viral types simultaneously. These markers could be applied when multiple source pollution is suspected, contributing to making decisions on public health interventions.
Fil: Barrios, Melina Elizabeth. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanco Fernandez, Maria Dolores. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cammarata, Robertina Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Torres, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Mbayed, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
BACTERIOPHAGES
BOVINE POLYOMAVIRUS
FOWL ADENOVIRUS
HUMAN POLYOMAVIRUS
INDICATORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99087
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99087
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewageBarrios, Melina ElizabethBlanco Fernandez, Maria DoloresCammarata, Robertina VivianaTorres, CarolinaMbayed, Viviana AndreaBACTERIOPHAGESBOVINE POLYOMAVIRUSFOWL ADENOVIRUSHUMAN POLYOMAVIRUSINDICATORShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants. The viable F-RNAPh count showed correlation with viable thermotolerant coliforms but also with human polyomaviruses (HPyV) quantified by a new molecular method. In domestic effluents, F-RNAPh and HPyV indicators significantly correlated with a human viral pathogen, norovirus, while the bacterial indicator did not, being then better predictors of the behavior of enteric pathogenic viruses. In addition, we assessed human, bovine and fowl microbial source tracking markers, based on the molecular detections of human polyomavirus, bovine polyomavirus, and fowl adenovirus, respectively. The techniques implemented extend the range of viruses detected, since they target different viral types simultaneously. These markers could be applied when multiple source pollution is suspected, contributing to making decisions on public health interventions.Fil: Barrios, Melina Elizabeth. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blanco Fernandez, Maria Dolores. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cammarata, Robertina Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Torres, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Mbayed, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaElsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99087Barrios, Melina Elizabeth; Blanco Fernandez, Maria Dolores; Cammarata, Robertina Viviana; Torres, Carolina; Mbayed, Viviana Andrea; Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage; Elsevier Science; Journal of Virological Methods; 262; 12-2018; 79-880166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S016609341830394Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2018.10.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:21Zoai:ri.conicet.gov.ar:11336/99087instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:22.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
title Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
spellingShingle Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
Barrios, Melina Elizabeth
BACTERIOPHAGES
BOVINE POLYOMAVIRUS
FOWL ADENOVIRUS
HUMAN POLYOMAVIRUS
INDICATORS
title_short Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
title_full Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
title_fullStr Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
title_full_unstemmed Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
title_sort Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage
dc.creator.none.fl_str_mv Barrios, Melina Elizabeth
Blanco Fernandez, Maria Dolores
Cammarata, Robertina Viviana
Torres, Carolina
Mbayed, Viviana Andrea
author Barrios, Melina Elizabeth
author_facet Barrios, Melina Elizabeth
Blanco Fernandez, Maria Dolores
Cammarata, Robertina Viviana
Torres, Carolina
Mbayed, Viviana Andrea
author_role author
author2 Blanco Fernandez, Maria Dolores
Cammarata, Robertina Viviana
Torres, Carolina
Mbayed, Viviana Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv BACTERIOPHAGES
BOVINE POLYOMAVIRUS
FOWL ADENOVIRUS
HUMAN POLYOMAVIRUS
INDICATORS
topic BACTERIOPHAGES
BOVINE POLYOMAVIRUS
FOWL ADENOVIRUS
HUMAN POLYOMAVIRUS
INDICATORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants. The viable F-RNAPh count showed correlation with viable thermotolerant coliforms but also with human polyomaviruses (HPyV) quantified by a new molecular method. In domestic effluents, F-RNAPh and HPyV indicators significantly correlated with a human viral pathogen, norovirus, while the bacterial indicator did not, being then better predictors of the behavior of enteric pathogenic viruses. In addition, we assessed human, bovine and fowl microbial source tracking markers, based on the molecular detections of human polyomavirus, bovine polyomavirus, and fowl adenovirus, respectively. The techniques implemented extend the range of viruses detected, since they target different viral types simultaneously. These markers could be applied when multiple source pollution is suspected, contributing to making decisions on public health interventions.
Fil: Barrios, Melina Elizabeth. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanco Fernandez, Maria Dolores. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cammarata, Robertina Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Torres, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Mbayed, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants. The viable F-RNAPh count showed correlation with viable thermotolerant coliforms but also with human polyomaviruses (HPyV) quantified by a new molecular method. In domestic effluents, F-RNAPh and HPyV indicators significantly correlated with a human viral pathogen, norovirus, while the bacterial indicator did not, being then better predictors of the behavior of enteric pathogenic viruses. In addition, we assessed human, bovine and fowl microbial source tracking markers, based on the molecular detections of human polyomavirus, bovine polyomavirus, and fowl adenovirus, respectively. The techniques implemented extend the range of viruses detected, since they target different viral types simultaneously. These markers could be applied when multiple source pollution is suspected, contributing to making decisions on public health interventions.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99087
Barrios, Melina Elizabeth; Blanco Fernandez, Maria Dolores; Cammarata, Robertina Viviana; Torres, Carolina; Mbayed, Viviana Andrea; Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage; Elsevier Science; Journal of Virological Methods; 262; 12-2018; 79-88
0166-0934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99087
identifier_str_mv Barrios, Melina Elizabeth; Blanco Fernandez, Maria Dolores; Cammarata, Robertina Viviana; Torres, Carolina; Mbayed, Viviana Andrea; Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage; Elsevier Science; Journal of Virological Methods; 262; 12-2018; 79-88
0166-0934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S016609341830394X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2018.10.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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