Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431

Autores
de Moreno, Maria Alejandra; Aragón, Félix Fernando; Carino, Silvia; Perdigón, Gabriela del Valle
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Breast cancer is the second cause of death in women, who are especially related to uncontrolled metastasis. It was previously demonstrated that the administration of milk fermented by Lactobacillus casei CRL 431 [fermented milk (FM)] delayed the tumor growth in a murine breast cancer model. In this work we evaluated if the administration of FM to mice, starting when the tumor was measurable, can affect not only the tumor growth, but also the extravasation of tumor cells and the lung metastasis. The evaluation of immune cells-infiltrating tumors and lungs was also performed. Tumor volume was calculated. Whole blood, lungs, and liver were processed to count the number of colonies formed by tumor cells. Blood serum was obtained for monocyte chemoattractant protein-1, interleukin (IL)-10, and IL-6 determination, lung tissues for histologic observations, and tumor tissues for angiogenesis determination. Mice that received FM were compared with animals given milk or to the controls without any especial supplementation. The results showed that FM administration to mice decreased or suppressed tumor growth, with less tumor vascularity, extravasation of tumor cells, and lung metastasis. These benefits were associated to modulation of the immune response by decreasing the infiltration of macrophages in both the tumor and the lungs. FM administration maintained an increased antitumor response associated to CD8 + lymphocytes, and also increased CD4 + lymphocytes that can be involved in the modulation of the immune response. The future evaluation of cytokine profiles will allow knowing more about subpopulation of macrophages and lymphocytes associated to the beneficial effect of this probiotic in the breast cancer model.
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Aragón, Félix Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Carino, Silvia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Perdigón, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Materia
BREAST CANCER
IMMUNE RESPONSE
METASTASIS
PROBIOTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3177
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3177
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431de Moreno, Maria AlejandraAragón, Félix FernandoCarino, SilviaPerdigón, Gabriela del ValleBREAST CANCERIMMUNE RESPONSEMETASTASISPROBIOTIChttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Breast cancer is the second cause of death in women, who are especially related to uncontrolled metastasis. It was previously demonstrated that the administration of milk fermented by Lactobacillus casei CRL 431 [fermented milk (FM)] delayed the tumor growth in a murine breast cancer model. In this work we evaluated if the administration of FM to mice, starting when the tumor was measurable, can affect not only the tumor growth, but also the extravasation of tumor cells and the lung metastasis. The evaluation of immune cells-infiltrating tumors and lungs was also performed. Tumor volume was calculated. Whole blood, lungs, and liver were processed to count the number of colonies formed by tumor cells. Blood serum was obtained for monocyte chemoattractant protein-1, interleukin (IL)-10, and IL-6 determination, lung tissues for histologic observations, and tumor tissues for angiogenesis determination. Mice that received FM were compared with animals given milk or to the controls without any especial supplementation. The results showed that FM administration to mice decreased or suppressed tumor growth, with less tumor vascularity, extravasation of tumor cells, and lung metastasis. These benefits were associated to modulation of the immune response by decreasing the infiltration of macrophages in both the tumor and the lungs. FM administration maintained an increased antitumor response associated to CD8 + lymphocytes, and also increased CD4 + lymphocytes that can be involved in the modulation of the immune response. The future evaluation of cytokine profiles will allow knowing more about subpopulation of macrophages and lymphocytes associated to the beneficial effect of this probiotic in the breast cancer model.Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Aragón, Félix Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Carino, Silvia. Universidad Nacional de Tucumán. Facultad de Odontología; ArgentinaFil: Perdigón, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaLippincott Williams2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3177de Moreno, Maria Alejandra; Aragón, Félix Fernando; Carino, Silvia; Perdigón, Gabriela del Valle; Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431; Lippincott Williams; Journal Of Immunotherapy; 38; 5; 6-2015; 185-1961524-95571537-4513enginfo:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/immunotherapy-journal/Abstract/2015/06000/Inhibition_of_Growth_and_Metastasis_of_Breast.2.aspxinfo:eu-repo/semantics/altIdentifier/doi/10.1097/CJI.0000000000000079info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:34Zoai:ri.conicet.gov.ar:11336/3177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:35.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
title Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
spellingShingle Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
de Moreno, Maria Alejandra
BREAST CANCER
IMMUNE RESPONSE
METASTASIS
PROBIOTIC
title_short Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
title_full Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
title_fullStr Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
title_full_unstemmed Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
title_sort Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431
dc.creator.none.fl_str_mv de Moreno, Maria Alejandra
Aragón, Félix Fernando
Carino, Silvia
Perdigón, Gabriela del Valle
author de Moreno, Maria Alejandra
author_facet de Moreno, Maria Alejandra
Aragón, Félix Fernando
Carino, Silvia
Perdigón, Gabriela del Valle
author_role author
author2 Aragón, Félix Fernando
Carino, Silvia
Perdigón, Gabriela del Valle
author2_role author
author
author
dc.subject.none.fl_str_mv BREAST CANCER
IMMUNE RESPONSE
METASTASIS
PROBIOTIC
topic BREAST CANCER
IMMUNE RESPONSE
METASTASIS
PROBIOTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Breast cancer is the second cause of death in women, who are especially related to uncontrolled metastasis. It was previously demonstrated that the administration of milk fermented by Lactobacillus casei CRL 431 [fermented milk (FM)] delayed the tumor growth in a murine breast cancer model. In this work we evaluated if the administration of FM to mice, starting when the tumor was measurable, can affect not only the tumor growth, but also the extravasation of tumor cells and the lung metastasis. The evaluation of immune cells-infiltrating tumors and lungs was also performed. Tumor volume was calculated. Whole blood, lungs, and liver were processed to count the number of colonies formed by tumor cells. Blood serum was obtained for monocyte chemoattractant protein-1, interleukin (IL)-10, and IL-6 determination, lung tissues for histologic observations, and tumor tissues for angiogenesis determination. Mice that received FM were compared with animals given milk or to the controls without any especial supplementation. The results showed that FM administration to mice decreased or suppressed tumor growth, with less tumor vascularity, extravasation of tumor cells, and lung metastasis. These benefits were associated to modulation of the immune response by decreasing the infiltration of macrophages in both the tumor and the lungs. FM administration maintained an increased antitumor response associated to CD8 + lymphocytes, and also increased CD4 + lymphocytes that can be involved in the modulation of the immune response. The future evaluation of cytokine profiles will allow knowing more about subpopulation of macrophages and lymphocytes associated to the beneficial effect of this probiotic in the breast cancer model.
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Aragón, Félix Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Carino, Silvia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina
Fil: Perdigón, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
description Breast cancer is the second cause of death in women, who are especially related to uncontrolled metastasis. It was previously demonstrated that the administration of milk fermented by Lactobacillus casei CRL 431 [fermented milk (FM)] delayed the tumor growth in a murine breast cancer model. In this work we evaluated if the administration of FM to mice, starting when the tumor was measurable, can affect not only the tumor growth, but also the extravasation of tumor cells and the lung metastasis. The evaluation of immune cells-infiltrating tumors and lungs was also performed. Tumor volume was calculated. Whole blood, lungs, and liver were processed to count the number of colonies formed by tumor cells. Blood serum was obtained for monocyte chemoattractant protein-1, interleukin (IL)-10, and IL-6 determination, lung tissues for histologic observations, and tumor tissues for angiogenesis determination. Mice that received FM were compared with animals given milk or to the controls without any especial supplementation. The results showed that FM administration to mice decreased or suppressed tumor growth, with less tumor vascularity, extravasation of tumor cells, and lung metastasis. These benefits were associated to modulation of the immune response by decreasing the infiltration of macrophages in both the tumor and the lungs. FM administration maintained an increased antitumor response associated to CD8 + lymphocytes, and also increased CD4 + lymphocytes that can be involved in the modulation of the immune response. The future evaluation of cytokine profiles will allow knowing more about subpopulation of macrophages and lymphocytes associated to the beneficial effect of this probiotic in the breast cancer model.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3177
de Moreno, Maria Alejandra; Aragón, Félix Fernando; Carino, Silvia; Perdigón, Gabriela del Valle; Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431; Lippincott Williams; Journal Of Immunotherapy; 38; 5; 6-2015; 185-196
1524-9557
1537-4513
url http://hdl.handle.net/11336/3177
identifier_str_mv de Moreno, Maria Alejandra; Aragón, Félix Fernando; Carino, Silvia; Perdigón, Gabriela del Valle; Inhibition of growth and metastasis of breast cancer in mice by milk fermented with Lactobacillus casei CRL 431; Lippincott Williams; Journal Of Immunotherapy; 38; 5; 6-2015; 185-196
1524-9557
1537-4513
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/immunotherapy-journal/Abstract/2015/06000/Inhibition_of_Growth_and_Metastasis_of_Breast.2.aspx
info:eu-repo/semantics/altIdentifier/doi/10.1097/CJI.0000000000000079
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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