Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism

Autores
Valles, Ana Sofia; Aveldaño, Marta Isabel; Furland, Natalia Edith
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Spermatogenesis is known to be vulnerable to temperature. Previous work from our group showed that in vivo testicular hyperthermia selectively damages germ cells at specific developmental stages and rapidly induces a seminiferous epithelium involution. This concurred with a considerable buildup of lipid droplets in Sertoli cells (SC). The aim of this study was to examine the effects of heat exposure on isolated SC in culture. Warming to 43ºC disrupted SC microtubule network and actin microfilaments. This SC cytoskeleton perturbation was accompanied by increased ROS production, mitochondrial depolarization, and accumulation of triacylglicerols (TAG). The heat exposure-dependent TAG accumulation could be explained by an impairment of mitochondrial fatty acid oxidation and a re-direction of fatty acids into TAG synthesis and lipid droplet formation. The impaired mitochondrial physiology also reduced the de novo synthesis of cardiolipin, as observed after incubating SC cultures with [3H]arachidonic acid. The hyperthermia-induced cytoskeletal and mitochondrial alterations could in part respond for the lipid metabolic disorder that SC undergo. Since these cells provide support to germ cells, these changes might be one of the underlying causes of the temporal impairment of spermatogenesis observed in vivo during testicular hyperthermia.
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Buenos Aires
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
SERTOLI CELL
HYPERTHERMIA
LIPID DROPLETS
CYTOSKELETON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234988
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolismValles, Ana SofiaAveldaño, Marta IsabelFurland, Natalia EdithSERTOLI CELLHYPERTHERMIALIPID DROPLETSCYTOSKELETONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Spermatogenesis is known to be vulnerable to temperature. Previous work from our group showed that in vivo testicular hyperthermia selectively damages germ cells at specific developmental stages and rapidly induces a seminiferous epithelium involution. This concurred with a considerable buildup of lipid droplets in Sertoli cells (SC). The aim of this study was to examine the effects of heat exposure on isolated SC in culture. Warming to 43ºC disrupted SC microtubule network and actin microfilaments. This SC cytoskeleton perturbation was accompanied by increased ROS production, mitochondrial depolarization, and accumulation of triacylglicerols (TAG). The heat exposure-dependent TAG accumulation could be explained by an impairment of mitochondrial fatty acid oxidation and a re-direction of fatty acids into TAG synthesis and lipid droplet formation. The impaired mitochondrial physiology also reduced the de novo synthesis of cardiolipin, as observed after incubating SC cultures with [3H]arachidonic acid. The hyperthermia-induced cytoskeletal and mitochondrial alterations could in part respond for the lipid metabolic disorder that SC undergo. Since these cells provide support to germ cells, these changes might be one of the underlying causes of the temporal impairment of spermatogenesis observed in vivo during testicular hyperthermia.Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularBuenos AiresArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularBiocell2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234988Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism; XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Buenos Aires; Argentina; 2013; 38-380327-95451667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-37.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:59Zoai:ri.conicet.gov.ar:11336/234988instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:00.091CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
title Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
spellingShingle Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
Valles, Ana Sofia
SERTOLI CELL
HYPERTHERMIA
LIPID DROPLETS
CYTOSKELETON
title_short Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
title_full Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
title_fullStr Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
title_full_unstemmed Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
title_sort Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism
dc.creator.none.fl_str_mv Valles, Ana Sofia
Aveldaño, Marta Isabel
Furland, Natalia Edith
author Valles, Ana Sofia
author_facet Valles, Ana Sofia
Aveldaño, Marta Isabel
Furland, Natalia Edith
author_role author
author2 Aveldaño, Marta Isabel
Furland, Natalia Edith
author2_role author
author
dc.subject.none.fl_str_mv SERTOLI CELL
HYPERTHERMIA
LIPID DROPLETS
CYTOSKELETON
topic SERTOLI CELL
HYPERTHERMIA
LIPID DROPLETS
CYTOSKELETON
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Spermatogenesis is known to be vulnerable to temperature. Previous work from our group showed that in vivo testicular hyperthermia selectively damages germ cells at specific developmental stages and rapidly induces a seminiferous epithelium involution. This concurred with a considerable buildup of lipid droplets in Sertoli cells (SC). The aim of this study was to examine the effects of heat exposure on isolated SC in culture. Warming to 43ºC disrupted SC microtubule network and actin microfilaments. This SC cytoskeleton perturbation was accompanied by increased ROS production, mitochondrial depolarization, and accumulation of triacylglicerols (TAG). The heat exposure-dependent TAG accumulation could be explained by an impairment of mitochondrial fatty acid oxidation and a re-direction of fatty acids into TAG synthesis and lipid droplet formation. The impaired mitochondrial physiology also reduced the de novo synthesis of cardiolipin, as observed after incubating SC cultures with [3H]arachidonic acid. The hyperthermia-induced cytoskeletal and mitochondrial alterations could in part respond for the lipid metabolic disorder that SC undergo. Since these cells provide support to germ cells, these changes might be one of the underlying causes of the temporal impairment of spermatogenesis observed in vivo during testicular hyperthermia.
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Buenos Aires
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Spermatogenesis is known to be vulnerable to temperature. Previous work from our group showed that in vivo testicular hyperthermia selectively damages germ cells at specific developmental stages and rapidly induces a seminiferous epithelium involution. This concurred with a considerable buildup of lipid droplets in Sertoli cells (SC). The aim of this study was to examine the effects of heat exposure on isolated SC in culture. Warming to 43ºC disrupted SC microtubule network and actin microfilaments. This SC cytoskeleton perturbation was accompanied by increased ROS production, mitochondrial depolarization, and accumulation of triacylglicerols (TAG). The heat exposure-dependent TAG accumulation could be explained by an impairment of mitochondrial fatty acid oxidation and a re-direction of fatty acids into TAG synthesis and lipid droplet formation. The impaired mitochondrial physiology also reduced the de novo synthesis of cardiolipin, as observed after incubating SC cultures with [3H]arachidonic acid. The hyperthermia-induced cytoskeletal and mitochondrial alterations could in part respond for the lipid metabolic disorder that SC undergo. Since these cells provide support to germ cells, these changes might be one of the underlying causes of the temporal impairment of spermatogenesis observed in vivo during testicular hyperthermia.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234988
Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism; XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Buenos Aires; Argentina; 2013; 38-38
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234988
identifier_str_mv Hyperthermia impairs Sertoli cell functions. Effects on lipid metabolism; XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Buenos Aires; Argentina; 2013; 38-38
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-37.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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