Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells

Autores
Valles, Ana Sofia; Furland, Natalia Edith; Aveldaño, Marta Isabel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Previous work demonstrated that exposures to mild hyperthermia results in altered lipid metabolism in cultured Sertoli cells (SC), as evidenced by accumulation of lipid droplets (LD), and that these changes concur with the disruption of SC cytoskeleton. To further investigate the relationship between cytoskeleton disruption and lipid perturbations, in this study SC cultures were exposed at a constant temperature to nocodazole (NCZ), an antineoplastic agent known to interfere with the polymerization of microtubules. As previously did hyperthermia, the cytoskeletal disarrangement induced by exposure to NCZ was accompanied by a significant alteration of the mitochondrial potential, an increase in triacylglycerol levels, a considerable accumulation of LD, and a functional cell impairment manifested in reduced expression of the SC-specific protein transferrin. As also seen after hyperthermia, the effects of NCZ on all these alterations were reverted after ending the exposures. The time-course of the changes suggest that the cytoskeletal disruption could be the primary cause of the SC mitochondrial alterations, which in turn may respond for the lipid metabolic alterations, suggestive of intracellular recycling of membrane lipid components. The cytoskeleton is thus functionally relevant in maintaining the viability and survival of SC during metabolically adverse or stressful situations.
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Córdoba
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
SERTOLI
CITOESQUELETO
MITOCONDRIA
GOTAS LIPÍDICAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230287
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cellsValles, Ana SofiaFurland, Natalia EdithAveldaño, Marta IsabelSERTOLICITOESQUELETOMITOCONDRIAGOTAS LIPÍDICAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previous work demonstrated that exposures to mild hyperthermia results in altered lipid metabolism in cultured Sertoli cells (SC), as evidenced by accumulation of lipid droplets (LD), and that these changes concur with the disruption of SC cytoskeleton. To further investigate the relationship between cytoskeleton disruption and lipid perturbations, in this study SC cultures were exposed at a constant temperature to nocodazole (NCZ), an antineoplastic agent known to interfere with the polymerization of microtubules. As previously did hyperthermia, the cytoskeletal disarrangement induced by exposure to NCZ was accompanied by a significant alteration of the mitochondrial potential, an increase in triacylglycerol levels, a considerable accumulation of LD, and a functional cell impairment manifested in reduced expression of the SC-specific protein transferrin. As also seen after hyperthermia, the effects of NCZ on all these alterations were reverted after ending the exposures. The time-course of the changes suggest that the cytoskeletal disruption could be the primary cause of the SC mitochondrial alterations, which in turn may respond for the lipid metabolic alterations, suggestive of intracellular recycling of membrane lipid components. The cytoskeleton is thus functionally relevant in maintaining the viability and survival of SC during metabolically adverse or stressful situations.Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularCórdobaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230287Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 105-1060327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v40nSuppl.S/34023/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:35Zoai:ri.conicet.gov.ar:11336/230287instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:35.876CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
title Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
spellingShingle Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
Valles, Ana Sofia
SERTOLI
CITOESQUELETO
MITOCONDRIA
GOTAS LIPÍDICAS
title_short Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
title_full Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
title_fullStr Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
title_full_unstemmed Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
title_sort Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells
dc.creator.none.fl_str_mv Valles, Ana Sofia
Furland, Natalia Edith
Aveldaño, Marta Isabel
author Valles, Ana Sofia
author_facet Valles, Ana Sofia
Furland, Natalia Edith
Aveldaño, Marta Isabel
author_role author
author2 Furland, Natalia Edith
Aveldaño, Marta Isabel
author2_role author
author
dc.subject.none.fl_str_mv SERTOLI
CITOESQUELETO
MITOCONDRIA
GOTAS LIPÍDICAS
topic SERTOLI
CITOESQUELETO
MITOCONDRIA
GOTAS LIPÍDICAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previous work demonstrated that exposures to mild hyperthermia results in altered lipid metabolism in cultured Sertoli cells (SC), as evidenced by accumulation of lipid droplets (LD), and that these changes concur with the disruption of SC cytoskeleton. To further investigate the relationship between cytoskeleton disruption and lipid perturbations, in this study SC cultures were exposed at a constant temperature to nocodazole (NCZ), an antineoplastic agent known to interfere with the polymerization of microtubules. As previously did hyperthermia, the cytoskeletal disarrangement induced by exposure to NCZ was accompanied by a significant alteration of the mitochondrial potential, an increase in triacylglycerol levels, a considerable accumulation of LD, and a functional cell impairment manifested in reduced expression of the SC-specific protein transferrin. As also seen after hyperthermia, the effects of NCZ on all these alterations were reverted after ending the exposures. The time-course of the changes suggest that the cytoskeletal disruption could be the primary cause of the SC mitochondrial alterations, which in turn may respond for the lipid metabolic alterations, suggestive of intracellular recycling of membrane lipid components. The cytoskeleton is thus functionally relevant in maintaining the viability and survival of SC during metabolically adverse or stressful situations.
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Córdoba
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Previous work demonstrated that exposures to mild hyperthermia results in altered lipid metabolism in cultured Sertoli cells (SC), as evidenced by accumulation of lipid droplets (LD), and that these changes concur with the disruption of SC cytoskeleton. To further investigate the relationship between cytoskeleton disruption and lipid perturbations, in this study SC cultures were exposed at a constant temperature to nocodazole (NCZ), an antineoplastic agent known to interfere with the polymerization of microtubules. As previously did hyperthermia, the cytoskeletal disarrangement induced by exposure to NCZ was accompanied by a significant alteration of the mitochondrial potential, an increase in triacylglycerol levels, a considerable accumulation of LD, and a functional cell impairment manifested in reduced expression of the SC-specific protein transferrin. As also seen after hyperthermia, the effects of NCZ on all these alterations were reverted after ending the exposures. The time-course of the changes suggest that the cytoskeletal disruption could be the primary cause of the SC mitochondrial alterations, which in turn may respond for the lipid metabolic alterations, suggestive of intracellular recycling of membrane lipid components. The cytoskeleton is thus functionally relevant in maintaining the viability and survival of SC during metabolically adverse or stressful situations.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230287
Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 105-106
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230287
identifier_str_mv Disruption of the cytoskeleton and altered lipid metabolism in Sertoli cells; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 105-106
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v40nSuppl.S/34023/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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