Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?

Autores
Aguilera Sammaritano, Juan Alberto; Andreas Gunter Reiner, Leclerque; Deymié Terzi, María Celina; Torrente, Karina A.; Vazquez, Fabio; Cuthbertson, Andrew; Lopez Lastra, Claudia Cristina; Lechner, Bernardo Ernesto
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Currently only monosporic strains from several entomopathogenic fungi have been selected and used for mass production and bioinsecticide manufacturing worldwide. The main reasons for the use of single spore instead of multispore strains are the attenuated virulence and the contaminants of the same species. In this study, different polisporic isolates and their monosporic combinations were tested against Tenebrio molitor (Linnaeus) larvae as an insect model. Isolates were obtained from arid soils. Four Metarhizium sp. (Metschn.) multisporic isolates (CEP413, CEP589, CEP590 and CEP591) were selected for bioassays. Trials were performed to evaluate mortality on three treatments, Full Polisporic (FP), Partial Polisporic (PP) and Pure Monosporic (PM). Cumulative mortality was measured at day 4 post infection. Sporulation percentage was assessed at day 6 post infection. The highest mortality was found at FP treatment (94%), the lowest mortality at day 4 was found at PM-CEP413 (32%). At day 6 the sporulation percentage was higher on FP (94%) and it was different from the rest of the treatments. To elucidate different polisporic and monosporic combinations to improve their effectiveness, may help to expand the use of bioinsecticides based on entomopathogenic fungi.
Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Andreas Gunter Reiner, Leclerque. Hochschule Geisenheim University; Alemania
Fil: Deymié Terzi, María Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Torrente, Karina A.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Cuthbertson, Andrew. Independent Science Advisor; Reino Unido
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
ENTOMOPATHOGENIC FUNGI
MONOSPORIC
POLISPORIC
METARHIZIUM SP
TENEBRIO MOLITOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53727
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53727
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?Aguilera Sammaritano, Juan AlbertoAndreas Gunter Reiner, LeclerqueDeymié Terzi, María CelinaTorrente, Karina A.Vazquez, FabioCuthbertson, AndrewLopez Lastra, Claudia CristinaLechner, Bernardo ErnestoENTOMOPATHOGENIC FUNGIMONOSPORICPOLISPORICMETARHIZIUM SPTENEBRIO MOLITORhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Currently only monosporic strains from several entomopathogenic fungi have been selected and used for mass production and bioinsecticide manufacturing worldwide. The main reasons for the use of single spore instead of multispore strains are the attenuated virulence and the contaminants of the same species. In this study, different polisporic isolates and their monosporic combinations were tested against Tenebrio molitor (Linnaeus) larvae as an insect model. Isolates were obtained from arid soils. Four Metarhizium sp. (Metschn.) multisporic isolates (CEP413, CEP589, CEP590 and CEP591) were selected for bioassays. Trials were performed to evaluate mortality on three treatments, Full Polisporic (FP), Partial Polisporic (PP) and Pure Monosporic (PM). Cumulative mortality was measured at day 4 post infection. Sporulation percentage was assessed at day 6 post infection. The highest mortality was found at FP treatment (94%), the lowest mortality at day 4 was found at PM-CEP413 (32%). At day 6 the sporulation percentage was higher on FP (94%) and it was different from the rest of the treatments. To elucidate different polisporic and monosporic combinations to improve their effectiveness, may help to expand the use of bioinsecticides based on entomopathogenic fungi.Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Andreas Gunter Reiner, Leclerque. Hochschule Geisenheim University; AlemaniaFil: Deymié Terzi, María Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Torrente, Karina A.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Cuthbertson, Andrew. Independent Science Advisor; Reino UnidoFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaSociedad Entomológica Argentina2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53727Aguilera Sammaritano, Juan Alberto; Andreas Gunter Reiner, Leclerque; Deymié Terzi, María Celina; Torrente, Karina A.; Vazquez, Fabio; et al.; Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?; Sociedad Entomológica Argentina; Revista de la Sociedad Entomológica Argentina; 76; 3-4; 12-2017; 39-430373-5680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.25085/rsea.763406info:eu-repo/semantics/altIdentifier/url/https://biotaxa.org/RSEA/article/view/35010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:13Zoai:ri.conicet.gov.ar:11336/53727instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:13.957CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
title Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
spellingShingle Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
Aguilera Sammaritano, Juan Alberto
ENTOMOPATHOGENIC FUNGI
MONOSPORIC
POLISPORIC
METARHIZIUM SP
TENEBRIO MOLITOR
title_short Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
title_full Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
title_fullStr Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
title_full_unstemmed Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
title_sort Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?
dc.creator.none.fl_str_mv Aguilera Sammaritano, Juan Alberto
Andreas Gunter Reiner, Leclerque
Deymié Terzi, María Celina
Torrente, Karina A.
Vazquez, Fabio
Cuthbertson, Andrew
Lopez Lastra, Claudia Cristina
Lechner, Bernardo Ernesto
author Aguilera Sammaritano, Juan Alberto
author_facet Aguilera Sammaritano, Juan Alberto
Andreas Gunter Reiner, Leclerque
Deymié Terzi, María Celina
Torrente, Karina A.
Vazquez, Fabio
Cuthbertson, Andrew
Lopez Lastra, Claudia Cristina
Lechner, Bernardo Ernesto
author_role author
author2 Andreas Gunter Reiner, Leclerque
Deymié Terzi, María Celina
Torrente, Karina A.
Vazquez, Fabio
Cuthbertson, Andrew
Lopez Lastra, Claudia Cristina
Lechner, Bernardo Ernesto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTOMOPATHOGENIC FUNGI
MONOSPORIC
POLISPORIC
METARHIZIUM SP
TENEBRIO MOLITOR
topic ENTOMOPATHOGENIC FUNGI
MONOSPORIC
POLISPORIC
METARHIZIUM SP
TENEBRIO MOLITOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Currently only monosporic strains from several entomopathogenic fungi have been selected and used for mass production and bioinsecticide manufacturing worldwide. The main reasons for the use of single spore instead of multispore strains are the attenuated virulence and the contaminants of the same species. In this study, different polisporic isolates and their monosporic combinations were tested against Tenebrio molitor (Linnaeus) larvae as an insect model. Isolates were obtained from arid soils. Four Metarhizium sp. (Metschn.) multisporic isolates (CEP413, CEP589, CEP590 and CEP591) were selected for bioassays. Trials were performed to evaluate mortality on three treatments, Full Polisporic (FP), Partial Polisporic (PP) and Pure Monosporic (PM). Cumulative mortality was measured at day 4 post infection. Sporulation percentage was assessed at day 6 post infection. The highest mortality was found at FP treatment (94%), the lowest mortality at day 4 was found at PM-CEP413 (32%). At day 6 the sporulation percentage was higher on FP (94%) and it was different from the rest of the treatments. To elucidate different polisporic and monosporic combinations to improve their effectiveness, may help to expand the use of bioinsecticides based on entomopathogenic fungi.
Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Andreas Gunter Reiner, Leclerque. Hochschule Geisenheim University; Alemania
Fil: Deymié Terzi, María Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Torrente, Karina A.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Cuthbertson, Andrew. Independent Science Advisor; Reino Unido
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description Currently only monosporic strains from several entomopathogenic fungi have been selected and used for mass production and bioinsecticide manufacturing worldwide. The main reasons for the use of single spore instead of multispore strains are the attenuated virulence and the contaminants of the same species. In this study, different polisporic isolates and their monosporic combinations were tested against Tenebrio molitor (Linnaeus) larvae as an insect model. Isolates were obtained from arid soils. Four Metarhizium sp. (Metschn.) multisporic isolates (CEP413, CEP589, CEP590 and CEP591) were selected for bioassays. Trials were performed to evaluate mortality on three treatments, Full Polisporic (FP), Partial Polisporic (PP) and Pure Monosporic (PM). Cumulative mortality was measured at day 4 post infection. Sporulation percentage was assessed at day 6 post infection. The highest mortality was found at FP treatment (94%), the lowest mortality at day 4 was found at PM-CEP413 (32%). At day 6 the sporulation percentage was higher on FP (94%) and it was different from the rest of the treatments. To elucidate different polisporic and monosporic combinations to improve their effectiveness, may help to expand the use of bioinsecticides based on entomopathogenic fungi.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53727
Aguilera Sammaritano, Juan Alberto; Andreas Gunter Reiner, Leclerque; Deymié Terzi, María Celina; Torrente, Karina A.; Vazquez, Fabio; et al.; Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?; Sociedad Entomológica Argentina; Revista de la Sociedad Entomológica Argentina; 76; 3-4; 12-2017; 39-43
0373-5680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53727
identifier_str_mv Aguilera Sammaritano, Juan Alberto; Andreas Gunter Reiner, Leclerque; Deymié Terzi, María Celina; Torrente, Karina A.; Vazquez, Fabio; et al.; Entomopathogenic fungi: Are polisporic isolates more pathogenic than monosporic strains?; Sociedad Entomológica Argentina; Revista de la Sociedad Entomológica Argentina; 76; 3-4; 12-2017; 39-43
0373-5680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.25085/rsea.763406
info:eu-repo/semantics/altIdentifier/url/https://biotaxa.org/RSEA/article/view/35010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Entomológica Argentina
publisher.none.fl_str_mv Sociedad Entomológica Argentina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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