A method to obtain monosporic cultures of arbuscular mycorrhizal fungi

Autores
Fracchia, Sebastian; Menendez, Ana Bernardina; Godeas, Alicia Margarita; Ocampo, J. A.
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible.
Fil: Fracchia, Sebastian. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Menendez, Ana Bernardina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Godeas, Alicia Margarita. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ocampo, J. A.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Materia
ARBUSCULAR MYCORRHIZAS
GIGASPORA
GLOMUS
MONOSPORIC CULTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163837
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163837
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A method to obtain monosporic cultures of arbuscular mycorrhizal fungiFracchia, SebastianMenendez, Ana BernardinaGodeas, Alicia MargaritaOcampo, J. A.ARBUSCULAR MYCORRHIZASGIGASPORAGLOMUSMONOSPORIC CULTUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible.Fil: Fracchia, Sebastian. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Menendez, Ana Bernardina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Godeas, Alicia Margarita. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ocampo, J. A.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaPergamon-Elsevier Science Ltd2001-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163837Fracchia, Sebastian; Menendez, Ana Bernardina; Godeas, Alicia Margarita; Ocampo, J. A.; A method to obtain monosporic cultures of arbuscular mycorrhizal fungi; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 33; 9; 7-2001; 1283-12850038-0717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0038071701000141info:eu-repo/semantics/altIdentifier/doi/10.1016/S0038-0717(01)00014-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:55Zoai:ri.conicet.gov.ar:11336/163837instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:56.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
spellingShingle A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
Fracchia, Sebastian
ARBUSCULAR MYCORRHIZAS
GIGASPORA
GLOMUS
MONOSPORIC CULTURE
title_short A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_full A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_fullStr A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_full_unstemmed A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
title_sort A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
dc.creator.none.fl_str_mv Fracchia, Sebastian
Menendez, Ana Bernardina
Godeas, Alicia Margarita
Ocampo, J. A.
author Fracchia, Sebastian
author_facet Fracchia, Sebastian
Menendez, Ana Bernardina
Godeas, Alicia Margarita
Ocampo, J. A.
author_role author
author2 Menendez, Ana Bernardina
Godeas, Alicia Margarita
Ocampo, J. A.
author2_role author
author
author
dc.subject.none.fl_str_mv ARBUSCULAR MYCORRHIZAS
GIGASPORA
GLOMUS
MONOSPORIC CULTURE
topic ARBUSCULAR MYCORRHIZAS
GIGASPORA
GLOMUS
MONOSPORIC CULTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible.
Fil: Fracchia, Sebastian. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Menendez, Ana Bernardina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Godeas, Alicia Margarita. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ocampo, J. A.. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
description We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible.
publishDate 2001
dc.date.none.fl_str_mv 2001-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163837
Fracchia, Sebastian; Menendez, Ana Bernardina; Godeas, Alicia Margarita; Ocampo, J. A.; A method to obtain monosporic cultures of arbuscular mycorrhizal fungi; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 33; 9; 7-2001; 1283-1285
0038-0717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163837
identifier_str_mv Fracchia, Sebastian; Menendez, Ana Bernardina; Godeas, Alicia Margarita; Ocampo, J. A.; A method to obtain monosporic cultures of arbuscular mycorrhizal fungi; Pergamon-Elsevier Science Ltd; Soil Biology And Biochemistry; 33; 9; 7-2001; 1283-1285
0038-0717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0038071701000141
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0038-0717(01)00014-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268633844154368
score 13.13397

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