Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina

Autores
Aguilera Sammaritano, Juan Alberto; Lopez Lastra, Claudia Cristina; Leclerque, Andreas; Vazquez, Fabio; Toro, Maria Eugenia; D´Alessandro, Celeste Paola; Cuthbertson, Andrew G.S.; Lechner, Bernardo Ernesto
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Entomopathogenic Hypocreales were isolated from arid soils in Argentina using Tenebrio molitor as bait and tested for their biological performance at 30°C and 45–65% RH. Conidial germination was tested in three vegetable oils (sunflower, olive and maize) at two concentrations (1% and 10%) to evaluate their compatibility for further liquid formulations. According to radial growth and germination results, we selected four isolates to test their pathogenicity against second instar B. tabaci nymphs with the selected oil formulations at 30°C. CEP381 and CEP401 showed the highest radial growth. Isolates CEP381, CEP401, CEP413 and CEP409 (Metarhizium spp.) had similar germination percentages as compared with water control when germinated on either sunflower, olive or maize oils at 10% v/v. The highest mortality of B. tabaci was observed for the isolates CEP381 in sunflower oil and CEP401 in olive oil. Molecular identification of isolates was performed using ITS4–5 primers. All isolates belong to the Metarhizium core group. Tested isolates could grow and infect B. tabaci nymphs at 30°C in some of the vegetable oils as carriers, providing new possibilities for integrated pest management of Bemisia tabaci.
Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Leclerque, Andreas. Hochschule Geisenheim University; Alemania
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: D´Alessandro, Celeste Paola. Universidade de Sao Paulo; Brasil
Fil: Cuthbertson, Andrew G.S.. Independent Science Advisor; Reino Unido
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
ENTOMOPATHOGENIC FUNGI
METARHIZIUM
LOW HUMIDITY
WHITEFLIES
OIL CONIDIA GERMINATION
PATHOGENICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109801
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/109801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in ArgentinaAguilera Sammaritano, Juan AlbertoLopez Lastra, Claudia CristinaLeclerque, AndreasVazquez, FabioToro, Maria EugeniaD´Alessandro, Celeste PaolaCuthbertson, Andrew G.S.Lechner, Bernardo ErnestoENTOMOPATHOGENIC FUNGIMETARHIZIUMLOW HUMIDITYWHITEFLIESOIL CONIDIA GERMINATIONPATHOGENICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Entomopathogenic Hypocreales were isolated from arid soils in Argentina using Tenebrio molitor as bait and tested for their biological performance at 30°C and 45–65% RH. Conidial germination was tested in three vegetable oils (sunflower, olive and maize) at two concentrations (1% and 10%) to evaluate their compatibility for further liquid formulations. According to radial growth and germination results, we selected four isolates to test their pathogenicity against second instar B. tabaci nymphs with the selected oil formulations at 30°C. CEP381 and CEP401 showed the highest radial growth. Isolates CEP381, CEP401, CEP413 and CEP409 (Metarhizium spp.) had similar germination percentages as compared with water control when germinated on either sunflower, olive or maize oils at 10% v/v. The highest mortality of B. tabaci was observed for the isolates CEP381 in sunflower oil and CEP401 in olive oil. Molecular identification of isolates was performed using ITS4–5 primers. All isolates belong to the Metarhizium core group. Tested isolates could grow and infect B. tabaci nymphs at 30°C in some of the vegetable oils as carriers, providing new possibilities for integrated pest management of Bemisia tabaci.Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Leclerque, Andreas. Hochschule Geisenheim University; AlemaniaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: D´Alessandro, Celeste Paola. Universidade de Sao Paulo; BrasilFil: Cuthbertson, Andrew G.S.. Independent Science Advisor; Reino UnidoFil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaTaylor & Francis Ltd2016-10-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109801Aguilera Sammaritano, Juan Alberto; Lopez Lastra, Claudia Cristina; Leclerque, Andreas; Vazquez, Fabio; Toro, Maria Eugenia; et al.; Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina; Taylor & Francis Ltd; Biocontrol Science and Technology; 26; 12; 18-10-2016; 1668-16820958-31571360-0478CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/09583157.2016.1231776info:eu-repo/semantics/altIdentifier/doi/10.1080/09583157.2016.1231776info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:28Zoai:ri.conicet.gov.ar:11336/109801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:29.144CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
title Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
spellingShingle Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
Aguilera Sammaritano, Juan Alberto
ENTOMOPATHOGENIC FUNGI
METARHIZIUM
LOW HUMIDITY
WHITEFLIES
OIL CONIDIA GERMINATION
PATHOGENICITY
title_short Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
title_full Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
title_fullStr Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
title_full_unstemmed Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
title_sort Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
dc.creator.none.fl_str_mv Aguilera Sammaritano, Juan Alberto
Lopez Lastra, Claudia Cristina
Leclerque, Andreas
Vazquez, Fabio
Toro, Maria Eugenia
D´Alessandro, Celeste Paola
Cuthbertson, Andrew G.S.
Lechner, Bernardo Ernesto
author Aguilera Sammaritano, Juan Alberto
author_facet Aguilera Sammaritano, Juan Alberto
Lopez Lastra, Claudia Cristina
Leclerque, Andreas
Vazquez, Fabio
Toro, Maria Eugenia
D´Alessandro, Celeste Paola
Cuthbertson, Andrew G.S.
Lechner, Bernardo Ernesto
author_role author
author2 Lopez Lastra, Claudia Cristina
Leclerque, Andreas
Vazquez, Fabio
Toro, Maria Eugenia
D´Alessandro, Celeste Paola
Cuthbertson, Andrew G.S.
Lechner, Bernardo Ernesto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTOMOPATHOGENIC FUNGI
METARHIZIUM
LOW HUMIDITY
WHITEFLIES
OIL CONIDIA GERMINATION
PATHOGENICITY
topic ENTOMOPATHOGENIC FUNGI
METARHIZIUM
LOW HUMIDITY
WHITEFLIES
OIL CONIDIA GERMINATION
PATHOGENICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Entomopathogenic Hypocreales were isolated from arid soils in Argentina using Tenebrio molitor as bait and tested for their biological performance at 30°C and 45–65% RH. Conidial germination was tested in three vegetable oils (sunflower, olive and maize) at two concentrations (1% and 10%) to evaluate their compatibility for further liquid formulations. According to radial growth and germination results, we selected four isolates to test their pathogenicity against second instar B. tabaci nymphs with the selected oil formulations at 30°C. CEP381 and CEP401 showed the highest radial growth. Isolates CEP381, CEP401, CEP413 and CEP409 (Metarhizium spp.) had similar germination percentages as compared with water control when germinated on either sunflower, olive or maize oils at 10% v/v. The highest mortality of B. tabaci was observed for the isolates CEP381 in sunflower oil and CEP401 in olive oil. Molecular identification of isolates was performed using ITS4–5 primers. All isolates belong to the Metarhizium core group. Tested isolates could grow and infect B. tabaci nymphs at 30°C in some of the vegetable oils as carriers, providing new possibilities for integrated pest management of Bemisia tabaci.
Fil: Aguilera Sammaritano, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Leclerque, Andreas. Hochschule Geisenheim University; Alemania
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: D´Alessandro, Celeste Paola. Universidade de Sao Paulo; Brasil
Fil: Cuthbertson, Andrew G.S.. Independent Science Advisor; Reino Unido
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description Entomopathogenic Hypocreales were isolated from arid soils in Argentina using Tenebrio molitor as bait and tested for their biological performance at 30°C and 45–65% RH. Conidial germination was tested in three vegetable oils (sunflower, olive and maize) at two concentrations (1% and 10%) to evaluate their compatibility for further liquid formulations. According to radial growth and germination results, we selected four isolates to test their pathogenicity against second instar B. tabaci nymphs with the selected oil formulations at 30°C. CEP381 and CEP401 showed the highest radial growth. Isolates CEP381, CEP401, CEP413 and CEP409 (Metarhizium spp.) had similar germination percentages as compared with water control when germinated on either sunflower, olive or maize oils at 10% v/v. The highest mortality of B. tabaci was observed for the isolates CEP381 in sunflower oil and CEP401 in olive oil. Molecular identification of isolates was performed using ITS4–5 primers. All isolates belong to the Metarhizium core group. Tested isolates could grow and infect B. tabaci nymphs at 30°C in some of the vegetable oils as carriers, providing new possibilities for integrated pest management of Bemisia tabaci.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109801
Aguilera Sammaritano, Juan Alberto; Lopez Lastra, Claudia Cristina; Leclerque, Andreas; Vazquez, Fabio; Toro, Maria Eugenia; et al.; Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina; Taylor & Francis Ltd; Biocontrol Science and Technology; 26; 12; 18-10-2016; 1668-1682
0958-3157
1360-0478
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109801
identifier_str_mv Aguilera Sammaritano, Juan Alberto; Lopez Lastra, Claudia Cristina; Leclerque, Andreas; Vazquez, Fabio; Toro, Maria Eugenia; et al.; Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina; Taylor & Francis Ltd; Biocontrol Science and Technology; 26; 12; 18-10-2016; 1668-1682
0958-3157
1360-0478
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/09583157.2016.1231776
info:eu-repo/semantics/altIdentifier/doi/10.1080/09583157.2016.1231776
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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