Effect of zinc on boar sperm liquid storage

Autores
Marini, Patricia Estela; Fernández Beato, Luciana; Cane, Fernando; Teijeiro, Juan Manuel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Storage and transport of liquid boar sperm for artificial insemination (AI) requires the addition of solutions called extenders, which increase the volume of the ejaculate and help preserve its functional characteristics. Yet, the quality of sperm decreases over time primarily due to the increased production of reactive oxygen species (ROS) that damage the plasma membrane. Many commercial extenders are supplemented with additives that mitigate this effect. In semen, zinc is supplied at high concentration on the seminal plasma and helps protect the plasma membrane of sperm. However, zinc in the seminal plasma is diluted and chelated upon addition of extenders for storage, potentially reducing its antioxidant effect. Here we characterize viability, motility, mitochondrial activity, DNA integrity and ROS content of boar sperm diluted with Sus (Medi Nova, Italy) extender supplemented with different concentrations of ZnCl2, at intervals after dilution during 3 days. The ability of sperm supplemented with 2 mM ZnCl2 to fertilize oocytes in vivo of was also tested. Sperm viability was over 82% for all treatments. Mitochondrial integrity analysis, measured by Cytochrome c activity, indicated a protector effect of Zn, noted as a reduced number of sperm with extensive loss of mitochondrial activity. Acrosomal integrity was improved by treatment with all concentrations of ZnCl2 tested. Sperm kinematics were affected by treatment with ZnCl2, showing higher percentage of progressive and rapid sperm in doses supplemented with 2mM ZnCl2. ROS levels and chromatin integrity did not show differences between ZnCl2-supplemented doses and the control. Fertilization rate, total number, live, still born and mummified piglets did not change when sperm were diluted with extender containing 2 mM ZnCl2. The presented characterization indicates that Zn addition to Sus extender have a protective effect on mitochondrial sheath and acrosomal membranes; and provides the basis for further studies aimed to optimize sperm performance in AI.
Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Fernández Beato, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina
Fil: Cane, Fernando. No especifíca;
Fil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Materia
ARTIFICIAL INSEMINATION
EXTENDER
PORCINE
SPERMATOZOA
ZINC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221233
Acceder
id CONICETDig_93dc07c2a96b01b326467e690fae923d
oai_identifier_str oai:ri.conicet.gov.ar:11336/221233
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of zinc on boar sperm liquid storageMarini, Patricia EstelaFernández Beato, LucianaCane, FernandoTeijeiro, Juan ManuelARTIFICIAL INSEMINATIONEXTENDERPORCINESPERMATOZOAZINChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Storage and transport of liquid boar sperm for artificial insemination (AI) requires the addition of solutions called extenders, which increase the volume of the ejaculate and help preserve its functional characteristics. Yet, the quality of sperm decreases over time primarily due to the increased production of reactive oxygen species (ROS) that damage the plasma membrane. Many commercial extenders are supplemented with additives that mitigate this effect. In semen, zinc is supplied at high concentration on the seminal plasma and helps protect the plasma membrane of sperm. However, zinc in the seminal plasma is diluted and chelated upon addition of extenders for storage, potentially reducing its antioxidant effect. Here we characterize viability, motility, mitochondrial activity, DNA integrity and ROS content of boar sperm diluted with Sus (Medi Nova, Italy) extender supplemented with different concentrations of ZnCl2, at intervals after dilution during 3 days. The ability of sperm supplemented with 2 mM ZnCl2 to fertilize oocytes in vivo of was also tested. Sperm viability was over 82% for all treatments. Mitochondrial integrity analysis, measured by Cytochrome c activity, indicated a protector effect of Zn, noted as a reduced number of sperm with extensive loss of mitochondrial activity. Acrosomal integrity was improved by treatment with all concentrations of ZnCl2 tested. Sperm kinematics were affected by treatment with ZnCl2, showing higher percentage of progressive and rapid sperm in doses supplemented with 2mM ZnCl2. ROS levels and chromatin integrity did not show differences between ZnCl2-supplemented doses and the control. Fertilization rate, total number, live, still born and mummified piglets did not change when sperm were diluted with extender containing 2 mM ZnCl2. The presented characterization indicates that Zn addition to Sus extender have a protective effect on mitochondrial sheath and acrosomal membranes; and provides the basis for further studies aimed to optimize sperm performance in AI.Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Fernández Beato, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; ArgentinaFil: Cane, Fernando. No especifíca;Fil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFrontiers Media2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221233Marini, Patricia Estela; Fernández Beato, Luciana; Cane, Fernando; Teijeiro, Juan Manuel; Effect of zinc on boar sperm liquid storage; Frontiers Media; Frontiers in Veterinary Science; 10; 2-2023; 1-112297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2023.1107929info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:30Zoai:ri.conicet.gov.ar:11336/221233instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:31.127CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of zinc on boar sperm liquid storage
title Effect of zinc on boar sperm liquid storage
spellingShingle Effect of zinc on boar sperm liquid storage
Marini, Patricia Estela
ARTIFICIAL INSEMINATION
EXTENDER
PORCINE
SPERMATOZOA
ZINC
title_short Effect of zinc on boar sperm liquid storage
title_full Effect of zinc on boar sperm liquid storage
title_fullStr Effect of zinc on boar sperm liquid storage
title_full_unstemmed Effect of zinc on boar sperm liquid storage
title_sort Effect of zinc on boar sperm liquid storage
dc.creator.none.fl_str_mv Marini, Patricia Estela
Fernández Beato, Luciana
Cane, Fernando
Teijeiro, Juan Manuel
author Marini, Patricia Estela
author_facet Marini, Patricia Estela
Fernández Beato, Luciana
Cane, Fernando
Teijeiro, Juan Manuel
author_role author
author2 Fernández Beato, Luciana
Cane, Fernando
Teijeiro, Juan Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv ARTIFICIAL INSEMINATION
EXTENDER
PORCINE
SPERMATOZOA
ZINC
topic ARTIFICIAL INSEMINATION
EXTENDER
PORCINE
SPERMATOZOA
ZINC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Storage and transport of liquid boar sperm for artificial insemination (AI) requires the addition of solutions called extenders, which increase the volume of the ejaculate and help preserve its functional characteristics. Yet, the quality of sperm decreases over time primarily due to the increased production of reactive oxygen species (ROS) that damage the plasma membrane. Many commercial extenders are supplemented with additives that mitigate this effect. In semen, zinc is supplied at high concentration on the seminal plasma and helps protect the plasma membrane of sperm. However, zinc in the seminal plasma is diluted and chelated upon addition of extenders for storage, potentially reducing its antioxidant effect. Here we characterize viability, motility, mitochondrial activity, DNA integrity and ROS content of boar sperm diluted with Sus (Medi Nova, Italy) extender supplemented with different concentrations of ZnCl2, at intervals after dilution during 3 days. The ability of sperm supplemented with 2 mM ZnCl2 to fertilize oocytes in vivo of was also tested. Sperm viability was over 82% for all treatments. Mitochondrial integrity analysis, measured by Cytochrome c activity, indicated a protector effect of Zn, noted as a reduced number of sperm with extensive loss of mitochondrial activity. Acrosomal integrity was improved by treatment with all concentrations of ZnCl2 tested. Sperm kinematics were affected by treatment with ZnCl2, showing higher percentage of progressive and rapid sperm in doses supplemented with 2mM ZnCl2. ROS levels and chromatin integrity did not show differences between ZnCl2-supplemented doses and the control. Fertilization rate, total number, live, still born and mummified piglets did not change when sperm were diluted with extender containing 2 mM ZnCl2. The presented characterization indicates that Zn addition to Sus extender have a protective effect on mitochondrial sheath and acrosomal membranes; and provides the basis for further studies aimed to optimize sperm performance in AI.
Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Fernández Beato, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina
Fil: Cane, Fernando. No especifíca;
Fil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
description Storage and transport of liquid boar sperm for artificial insemination (AI) requires the addition of solutions called extenders, which increase the volume of the ejaculate and help preserve its functional characteristics. Yet, the quality of sperm decreases over time primarily due to the increased production of reactive oxygen species (ROS) that damage the plasma membrane. Many commercial extenders are supplemented with additives that mitigate this effect. In semen, zinc is supplied at high concentration on the seminal plasma and helps protect the plasma membrane of sperm. However, zinc in the seminal plasma is diluted and chelated upon addition of extenders for storage, potentially reducing its antioxidant effect. Here we characterize viability, motility, mitochondrial activity, DNA integrity and ROS content of boar sperm diluted with Sus (Medi Nova, Italy) extender supplemented with different concentrations of ZnCl2, at intervals after dilution during 3 days. The ability of sperm supplemented with 2 mM ZnCl2 to fertilize oocytes in vivo of was also tested. Sperm viability was over 82% for all treatments. Mitochondrial integrity analysis, measured by Cytochrome c activity, indicated a protector effect of Zn, noted as a reduced number of sperm with extensive loss of mitochondrial activity. Acrosomal integrity was improved by treatment with all concentrations of ZnCl2 tested. Sperm kinematics were affected by treatment with ZnCl2, showing higher percentage of progressive and rapid sperm in doses supplemented with 2mM ZnCl2. ROS levels and chromatin integrity did not show differences between ZnCl2-supplemented doses and the control. Fertilization rate, total number, live, still born and mummified piglets did not change when sperm were diluted with extender containing 2 mM ZnCl2. The presented characterization indicates that Zn addition to Sus extender have a protective effect on mitochondrial sheath and acrosomal membranes; and provides the basis for further studies aimed to optimize sperm performance in AI.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221233
Marini, Patricia Estela; Fernández Beato, Luciana; Cane, Fernando; Teijeiro, Juan Manuel; Effect of zinc on boar sperm liquid storage; Frontiers Media; Frontiers in Veterinary Science; 10; 2-2023; 1-11
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221233
identifier_str_mv Marini, Patricia Estela; Fernández Beato, Luciana; Cane, Fernando; Teijeiro, Juan Manuel; Effect of zinc on boar sperm liquid storage; Frontiers Media; Frontiers in Veterinary Science; 10; 2-2023; 1-11
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2023.1107929
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782653054844928
score 12.982451

Publicaciones similares