In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles

Autores
Garrappa, Gabriela; Martínez López, Cristina; Jiménez Movilla, María; García Vázquez, Francisco Alberto
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain
Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in vitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, although some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, in vitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.
EEA Rafaela
Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Garrappa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Martínez López, Cristina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Jimenez Movilla, Maria. Instituto Murciano de Investigación Biosanitaria (IMIB); España
Fil: Jimenez Movilla, Maria. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España
Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España
Fuente
Reproduction in Domestic Animals 59 (S3) : e14654. (October 2024)
Materia
Tecnología
Cerdo
Espermatozoo
Nanobiotecnología
Technology
Swine
Spermatozoa
Nanobiotechnology
Porcinos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticlesGarrappa, GabrielaMartínez López, CristinaJiménez Movilla, MaríaGarcía Vázquez, Francisco AlbertoTecnologíaCerdoEspermatozooNanobiotecnologíaTechnologySwineSpermatozoaNanobiotechnologyPorcinosSupplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, SpainNanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in vitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, although some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, in vitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.EEA RafaelaFil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Garrappa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Martínez López, Cristina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: Jimenez Movilla, Maria. Instituto Murciano de Investigación Biosanitaria (IMIB); EspañaFil: Jimenez Movilla, Maria. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; EspañaFil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); EspañaWiley2025-05-09T16:20:20Z2025-05-09T16:20:20Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22225https://onlinelibrary.wiley.com/doi/10.1111/rda.146540936-67681439-0531https://doi.org/10.1111/rda.14654Reproduction in Domestic Animals 59 (S3) : e14654. (October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-06T09:42:41Zoai:localhost:20.500.12123/22225instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:42:41.448INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
title In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
spellingShingle In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
Garrappa, Gabriela
Tecnología
Cerdo
Espermatozoo
Nanobiotecnología
Technology
Swine
Spermatozoa
Nanobiotechnology
Porcinos
title_short In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
title_full In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
title_fullStr In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
title_full_unstemmed In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
title_sort In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
dc.creator.none.fl_str_mv Garrappa, Gabriela
Martínez López, Cristina
Jiménez Movilla, María
García Vázquez, Francisco Alberto
author Garrappa, Gabriela
author_facet Garrappa, Gabriela
Martínez López, Cristina
Jiménez Movilla, María
García Vázquez, Francisco Alberto
author_role author
author2 Martínez López, Cristina
Jiménez Movilla, María
García Vázquez, Francisco Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Tecnología
Cerdo
Espermatozoo
Nanobiotecnología
Technology
Swine
Spermatozoa
Nanobiotechnology
Porcinos
topic Tecnología
Cerdo
Espermatozoo
Nanobiotecnología
Technology
Swine
Spermatozoa
Nanobiotechnology
Porcinos
dc.description.none.fl_txt_mv Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain
Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in vitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, although some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, in vitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.
EEA Rafaela
Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Garrappa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Martínez López, Cristina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Jimenez Movilla, Maria. Instituto Murciano de Investigación Biosanitaria (IMIB); España
Fil: Jimenez Movilla, Maria. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España
Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España
description Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain
publishDate 2024
dc.date.none.fl_str_mv 2024-10
2025-05-09T16:20:20Z
2025-05-09T16:20:20Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22225
https://onlinelibrary.wiley.com/doi/10.1111/rda.14654
0936-6768
1439-0531
https://doi.org/10.1111/rda.14654
url http://hdl.handle.net/20.500.12123/22225
https://onlinelibrary.wiley.com/doi/10.1111/rda.14654
https://doi.org/10.1111/rda.14654
identifier_str_mv 0936-6768
1439-0531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Reproduction in Domestic Animals 59 (S3) : e14654. (October 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.087074

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