Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples

Autores
Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernandez, Hector; Zon, María Alicia; Molina, Patricia Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study describes the development of a very sensitive electrochemical immunosensor (EI) for the determination of 17β-estradiol (17β-E). The novelty of this immunosensor is that the detection of 17β-E is carried out without sample pretreatment and unlabeled neither the antigen nor the antibody. Very good results in terms of sensitivity, kinetics, and working range are obtained. The immunosensor was constructed by immobilization of the anti-17β-E monoclonal antibody (mAbE) on a gold disk electrode modified with gold nanoparticles on a cysteamine self-assembled monolayer (AuNP-cys-Au disk). Bovine serum samples were spiked with known amounts of 17β-E and incubated on mAbE-AuNP-cys-Au disk electrodes. Then, the EI was transferred to pH 5.00 citrate buffer solutions, containing horseradish peroxidase (HRP), pyrocatechol (H2Q), and H2O2 at given concentrations. The 17β-E and H2Q, both enzyme co-substrates, react with HRP. The HRP, which did not react with 17β-E, in the presence of H2O2 catalyzes the oxidation of H2Q to o-benzoquinone (Q). The back electrochemical reduction of Q to H2Q was detected on the modified gold electrode surface by square wave voltammetry. The electrochemical signal was proportional to the amount of H2Q that reacts with the enzyme, and inversely proportional to the amount of 17β-E presents in the bovine serum samples. The EI showed a linear range from 0.54 to 1.36 × 104 pg mL-1. The limit of detection (LOD) was 0.84 pg mL-1. Recovery percentages were very good, with values of 99.7, 106, and 105% for 10, 50 and 100 pg mL-1, respectively. This EI is an attractive tool for the 17β-E determination in bovine serum samples.
Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
17Β-Estradiol
Enzyme Immunoassays
Gold Nanoparticles
Immunosensor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37621
Acceder
id CONICETDig_0da075ecff262eb66ce725098c92801d
oai_identifier_str oai:ri.conicet.gov.ar:11336/37621
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samplesMonerris, Melisa JimenaArevalo, Fernando JavierFernandez, HectorZon, María AliciaMolina, Patricia Gabriela17Β-EstradiolEnzyme ImmunoassaysGold NanoparticlesImmunosensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This study describes the development of a very sensitive electrochemical immunosensor (EI) for the determination of 17β-estradiol (17β-E). The novelty of this immunosensor is that the detection of 17β-E is carried out without sample pretreatment and unlabeled neither the antigen nor the antibody. Very good results in terms of sensitivity, kinetics, and working range are obtained. The immunosensor was constructed by immobilization of the anti-17β-E monoclonal antibody (mAbE) on a gold disk electrode modified with gold nanoparticles on a cysteamine self-assembled monolayer (AuNP-cys-Au disk). Bovine serum samples were spiked with known amounts of 17β-E and incubated on mAbE-AuNP-cys-Au disk electrodes. Then, the EI was transferred to pH 5.00 citrate buffer solutions, containing horseradish peroxidase (HRP), pyrocatechol (H2Q), and H2O2 at given concentrations. The 17β-E and H2Q, both enzyme co-substrates, react with HRP. The HRP, which did not react with 17β-E, in the presence of H2O2 catalyzes the oxidation of H2Q to o-benzoquinone (Q). The back electrochemical reduction of Q to H2Q was detected on the modified gold electrode surface by square wave voltammetry. The electrochemical signal was proportional to the amount of H2Q that reacts with the enzyme, and inversely proportional to the amount of 17β-E presents in the bovine serum samples. The EI showed a linear range from 0.54 to 1.36 × 104 pg mL-1. The limit of detection (LOD) was 0.84 pg mL-1. Recovery percentages were very good, with values of 99.7, 106, and 105% for 10, 50 and 100 pg mL-1, respectively. This EI is an attractive tool for the 17β-E determination in bovine serum samples.Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Sa2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37621Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernandez, Hector; Zon, María Alicia; Molina, Patricia Gabriela; Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples; Elsevier Science Sa; Sensors and Actuators B: Chemical; 208; 3-2015; 525-5310925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.11.048info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092540051401418Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:35Zoai:ri.conicet.gov.ar:11336/37621instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:35.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
title Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
spellingShingle Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
Monerris, Melisa Jimena
17Β-Estradiol
Enzyme Immunoassays
Gold Nanoparticles
Immunosensor
title_short Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
title_full Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
title_fullStr Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
title_full_unstemmed Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
title_sort Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
dc.creator.none.fl_str_mv Monerris, Melisa Jimena
Arevalo, Fernando Javier
Fernandez, Hector
Zon, María Alicia
Molina, Patricia Gabriela
author Monerris, Melisa Jimena
author_facet Monerris, Melisa Jimena
Arevalo, Fernando Javier
Fernandez, Hector
Zon, María Alicia
Molina, Patricia Gabriela
author_role author
author2 Arevalo, Fernando Javier
Fernandez, Hector
Zon, María Alicia
Molina, Patricia Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv 17Β-Estradiol
Enzyme Immunoassays
Gold Nanoparticles
Immunosensor
topic 17Β-Estradiol
Enzyme Immunoassays
Gold Nanoparticles
Immunosensor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study describes the development of a very sensitive electrochemical immunosensor (EI) for the determination of 17β-estradiol (17β-E). The novelty of this immunosensor is that the detection of 17β-E is carried out without sample pretreatment and unlabeled neither the antigen nor the antibody. Very good results in terms of sensitivity, kinetics, and working range are obtained. The immunosensor was constructed by immobilization of the anti-17β-E monoclonal antibody (mAbE) on a gold disk electrode modified with gold nanoparticles on a cysteamine self-assembled monolayer (AuNP-cys-Au disk). Bovine serum samples were spiked with known amounts of 17β-E and incubated on mAbE-AuNP-cys-Au disk electrodes. Then, the EI was transferred to pH 5.00 citrate buffer solutions, containing horseradish peroxidase (HRP), pyrocatechol (H2Q), and H2O2 at given concentrations. The 17β-E and H2Q, both enzyme co-substrates, react with HRP. The HRP, which did not react with 17β-E, in the presence of H2O2 catalyzes the oxidation of H2Q to o-benzoquinone (Q). The back electrochemical reduction of Q to H2Q was detected on the modified gold electrode surface by square wave voltammetry. The electrochemical signal was proportional to the amount of H2Q that reacts with the enzyme, and inversely proportional to the amount of 17β-E presents in the bovine serum samples. The EI showed a linear range from 0.54 to 1.36 × 104 pg mL-1. The limit of detection (LOD) was 0.84 pg mL-1. Recovery percentages were very good, with values of 99.7, 106, and 105% for 10, 50 and 100 pg mL-1, respectively. This EI is an attractive tool for the 17β-E determination in bovine serum samples.
Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description This study describes the development of a very sensitive electrochemical immunosensor (EI) for the determination of 17β-estradiol (17β-E). The novelty of this immunosensor is that the detection of 17β-E is carried out without sample pretreatment and unlabeled neither the antigen nor the antibody. Very good results in terms of sensitivity, kinetics, and working range are obtained. The immunosensor was constructed by immobilization of the anti-17β-E monoclonal antibody (mAbE) on a gold disk electrode modified with gold nanoparticles on a cysteamine self-assembled monolayer (AuNP-cys-Au disk). Bovine serum samples were spiked with known amounts of 17β-E and incubated on mAbE-AuNP-cys-Au disk electrodes. Then, the EI was transferred to pH 5.00 citrate buffer solutions, containing horseradish peroxidase (HRP), pyrocatechol (H2Q), and H2O2 at given concentrations. The 17β-E and H2Q, both enzyme co-substrates, react with HRP. The HRP, which did not react with 17β-E, in the presence of H2O2 catalyzes the oxidation of H2Q to o-benzoquinone (Q). The back electrochemical reduction of Q to H2Q was detected on the modified gold electrode surface by square wave voltammetry. The electrochemical signal was proportional to the amount of H2Q that reacts with the enzyme, and inversely proportional to the amount of 17β-E presents in the bovine serum samples. The EI showed a linear range from 0.54 to 1.36 × 104 pg mL-1. The limit of detection (LOD) was 0.84 pg mL-1. Recovery percentages were very good, with values of 99.7, 106, and 105% for 10, 50 and 100 pg mL-1, respectively. This EI is an attractive tool for the 17β-E determination in bovine serum samples.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37621
Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernandez, Hector; Zon, María Alicia; Molina, Patricia Gabriela; Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples; Elsevier Science Sa; Sensors and Actuators B: Chemical; 208; 3-2015; 525-531
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37621
identifier_str_mv Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernandez, Hector; Zon, María Alicia; Molina, Patricia Gabriela; Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples; Elsevier Science Sa; Sensors and Actuators B: Chemical; 208; 3-2015; 525-531
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.11.048
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092540051401418X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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