Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators

Autores
Alayón Luaces, Paula; Ponce, Nora Marta Andrea; Mroginski, Luis Amado; Stortz, Carlos Arturo; Sozzi, Gabriel Oscar
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cell wall composition of apples callus cultures showed changes in the presence of 5 mg l−1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the total pectin and matrix glycan content, this work provides evidence that the addition of these plant growth regulators can rule, at least partly, cell wall metabolism in apple callus cultures. The chelator- and carbonate-extracts always had the analytical characteristics of pectins, with high proportions of uronic acids, arabinose and galactose as the main monosaccharides, and a significant proportion of rhamnose, but the cross-linking glycan fractions were still rich in RG-I-like material. The application of PGRs produced shifts of uronic acid and neutral sugars between fractions. Arabinose was the neutral sugar exhibiting more variations in apple callus cell wall. Picloram and abscisic acid produced an increase of the uronic acid contents of the cell walls. The AIRs obtained from calluses treated with different PGRs did not show large amounts of high molecular weight products, as determined by size-exclusion chromatography. For the carbonate-extract only the callus treated with picloram displayed two separated peaks for products of different molecular weights. The chromatographic profiles for the 4% KOH-extract displayed two peaks for all the treatments, one very sharp with high molecular weight, and another one wider of smaller molecular weight, whereas the difference between treatments can only be appraised through the areas of the peaks. This is the first report on cell wall composition from fruit calluses supplemented with different PGRs.
Fil: Alayón Luaces, Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Ponce, Nora Marta Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Stortz, Carlos Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Sozzi, Gabriel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Callus culture
Cell wall
Cross-linking glycan
Malus domestica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241805
Acceder
id CONICETDig_935049bf50c9fcd331b5050f512feefc
oai_identifier_str oai:ri.conicet.gov.ar:11336/241805
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulatorsAlayón Luaces, PaulaPonce, Nora Marta AndreaMroginski, Luis AmadoStortz, Carlos ArturoSozzi, Gabriel OscarCallus cultureCell wallCross-linking glycanMalus domesticahttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The cell wall composition of apples callus cultures showed changes in the presence of 5 mg l−1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the total pectin and matrix glycan content, this work provides evidence that the addition of these plant growth regulators can rule, at least partly, cell wall metabolism in apple callus cultures. The chelator- and carbonate-extracts always had the analytical characteristics of pectins, with high proportions of uronic acids, arabinose and galactose as the main monosaccharides, and a significant proportion of rhamnose, but the cross-linking glycan fractions were still rich in RG-I-like material. The application of PGRs produced shifts of uronic acid and neutral sugars between fractions. Arabinose was the neutral sugar exhibiting more variations in apple callus cell wall. Picloram and abscisic acid produced an increase of the uronic acid contents of the cell walls. The AIRs obtained from calluses treated with different PGRs did not show large amounts of high molecular weight products, as determined by size-exclusion chromatography. For the carbonate-extract only the callus treated with picloram displayed two separated peaks for products of different molecular weights. The chromatographic profiles for the 4% KOH-extract displayed two peaks for all the treatments, one very sharp with high molecular weight, and another one wider of smaller molecular weight, whereas the difference between treatments can only be appraised through the areas of the peaks. This is the first report on cell wall composition from fruit calluses supplemented with different PGRs.Fil: Alayón Luaces, Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Ponce, Nora Marta Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Stortz, Carlos Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Sozzi, Gabriel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241805Alayón Luaces, Paula; Ponce, Nora Marta Andrea; Mroginski, Luis Amado; Stortz, Carlos Arturo; Sozzi, Gabriel Oscar; Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators; Elsevier Ireland; Plant Science; 185-186; 4-2012; 169-1750168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945211002950info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2011.10.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:20:44Zoai:ri.conicet.gov.ar:11336/241805instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:20:45.075CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
title Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
spellingShingle Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
Alayón Luaces, Paula
Callus culture
Cell wall
Cross-linking glycan
Malus domestica
title_short Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
title_full Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
title_fullStr Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
title_full_unstemmed Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
title_sort Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators
dc.creator.none.fl_str_mv Alayón Luaces, Paula
Ponce, Nora Marta Andrea
Mroginski, Luis Amado
Stortz, Carlos Arturo
Sozzi, Gabriel Oscar
author Alayón Luaces, Paula
author_facet Alayón Luaces, Paula
Ponce, Nora Marta Andrea
Mroginski, Luis Amado
Stortz, Carlos Arturo
Sozzi, Gabriel Oscar
author_role author
author2 Ponce, Nora Marta Andrea
Mroginski, Luis Amado
Stortz, Carlos Arturo
Sozzi, Gabriel Oscar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Callus culture
Cell wall
Cross-linking glycan
Malus domestica
topic Callus culture
Cell wall
Cross-linking glycan
Malus domestica
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The cell wall composition of apples callus cultures showed changes in the presence of 5 mg l−1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the total pectin and matrix glycan content, this work provides evidence that the addition of these plant growth regulators can rule, at least partly, cell wall metabolism in apple callus cultures. The chelator- and carbonate-extracts always had the analytical characteristics of pectins, with high proportions of uronic acids, arabinose and galactose as the main monosaccharides, and a significant proportion of rhamnose, but the cross-linking glycan fractions were still rich in RG-I-like material. The application of PGRs produced shifts of uronic acid and neutral sugars between fractions. Arabinose was the neutral sugar exhibiting more variations in apple callus cell wall. Picloram and abscisic acid produced an increase of the uronic acid contents of the cell walls. The AIRs obtained from calluses treated with different PGRs did not show large amounts of high molecular weight products, as determined by size-exclusion chromatography. For the carbonate-extract only the callus treated with picloram displayed two separated peaks for products of different molecular weights. The chromatographic profiles for the 4% KOH-extract displayed two peaks for all the treatments, one very sharp with high molecular weight, and another one wider of smaller molecular weight, whereas the difference between treatments can only be appraised through the areas of the peaks. This is the first report on cell wall composition from fruit calluses supplemented with different PGRs.
Fil: Alayón Luaces, Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Ponce, Nora Marta Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Stortz, Carlos Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Sozzi, Gabriel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The cell wall composition of apples callus cultures showed changes in the presence of 5 mg l−1 of three different plant growth regulators (PGRs), namely picloram, abscisic acid and gibberellic acid. Although the structural functions of cell walls do not generally allow for pronounced variations of the total pectin and matrix glycan content, this work provides evidence that the addition of these plant growth regulators can rule, at least partly, cell wall metabolism in apple callus cultures. The chelator- and carbonate-extracts always had the analytical characteristics of pectins, with high proportions of uronic acids, arabinose and galactose as the main monosaccharides, and a significant proportion of rhamnose, but the cross-linking glycan fractions were still rich in RG-I-like material. The application of PGRs produced shifts of uronic acid and neutral sugars between fractions. Arabinose was the neutral sugar exhibiting more variations in apple callus cell wall. Picloram and abscisic acid produced an increase of the uronic acid contents of the cell walls. The AIRs obtained from calluses treated with different PGRs did not show large amounts of high molecular weight products, as determined by size-exclusion chromatography. For the carbonate-extract only the callus treated with picloram displayed two separated peaks for products of different molecular weights. The chromatographic profiles for the 4% KOH-extract displayed two peaks for all the treatments, one very sharp with high molecular weight, and another one wider of smaller molecular weight, whereas the difference between treatments can only be appraised through the areas of the peaks. This is the first report on cell wall composition from fruit calluses supplemented with different PGRs.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241805
Alayón Luaces, Paula; Ponce, Nora Marta Andrea; Mroginski, Luis Amado; Stortz, Carlos Arturo; Sozzi, Gabriel Oscar; Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators; Elsevier Ireland; Plant Science; 185-186; 4-2012; 169-175
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241805
identifier_str_mv Alayón Luaces, Paula; Ponce, Nora Marta Andrea; Mroginski, Luis Amado; Stortz, Carlos Arturo; Sozzi, Gabriel Oscar; Compositional changes in cell wall polysaccharides from apple fruit callus cultures modulated by different plant growth regulators; Elsevier Ireland; Plant Science; 185-186; 4-2012; 169-175
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945211002950
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2011.10.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606553252331520
score 13.001348

Publicaciones similares