Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition

Autores
Parsons, J.; Wirth, Sonia Alejandra; Dominguez, M.; Bravo Almonacid, Fernando Felix; Giulietti, Ana Maria; Rodriguez Talou, Julian
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to study the production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum. Suspension and hairy root cultures expressing hEGF were established and the time course of growth and hEGF production were evaluated. In hairy root cultures, hEGF content was about 2 µg g -1FW, with a productivity of 15.9 µg l-1d-1 whereas in cell suspensions, a specific production of 0.48-0.53 µg g -1FW and a productivity of 31 µg l-1 d-1 were obtained. From these results, it is clear that hEGF synthesis and accumulation depend on tissue differentiation. In order to increase hEGF production, cell suspension cultures were treated with casaminoacids and sodium nitroprusside (SNP, a nitric oxide donor). SNP treatment resulted in an increase of hEGF productivity of 48% compared with control cultures. In conclusion, cell suspension and hairy root cultures established from transgenic N. tabacum were able to produce hEGF and the concentration and yield obtained depended on the level of tissue differentiation and the treatment applied.
Fil: Parsons, J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Dominguez, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Giulietti, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez Talou, Julian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Human Epidermal Growth Factor;
Nicotiana Tabacum
Suspension Cultures
Hairy Roots
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15359
Acceder
id CONICETDig_276d86c6be5e7c147d3b64f11413e3a1
oai_identifier_str oai:ri.conicet.gov.ar:11336/15359
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside additionProduction of Human epidermal growth factor (hegf) nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside additionParsons, J.Wirth, Sonia AlejandraDominguez, M.Bravo Almonacid, Fernando FelixGiulietti, Ana MariaRodriguez Talou, JulianHuman Epidermal Growth Factor;Nicotiana TabacumSuspension CulturesHairy Rootshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The aim of this work was to study the production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum. Suspension and hairy root cultures expressing hEGF were established and the time course of growth and hEGF production were evaluated. In hairy root cultures, hEGF content was about 2 µg g -1FW, with a productivity of 15.9 µg l-1d-1 whereas in cell suspensions, a specific production of 0.48-0.53 µg g -1FW and a productivity of 31 µg l-1 d-1 were obtained. From these results, it is clear that hEGF synthesis and accumulation depend on tissue differentiation. In order to increase hEGF production, cell suspension cultures were treated with casaminoacids and sodium nitroprusside (SNP, a nitric oxide donor). SNP treatment resulted in an increase of hEGF productivity of 48% compared with control cultures. In conclusion, cell suspension and hairy root cultures established from transgenic N. tabacum were able to produce hEGF and the concentration and yield obtained depended on the level of tissue differentiation and the treatment applied.Fil: Parsons, J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Dominguez, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; ArgentinaFil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Giulietti, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez Talou, Julian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaResearch India2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15359Parsons, J.; Wirth, Sonia Alejandra; Dominguez, M.; Bravo Almonacid, Fernando Felix; Giulietti, Ana Maria; et al.; Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition; Research India; International Journal of Biotechnology and Biochemistry; 6; 1; 12-2010; 131-1380973-26910974-4762enginfo:eu-repo/semantics/altIdentifier/url/http://www.ripublication.com/Volume/ijbbv6n1.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:47Zoai:ri.conicet.gov.ar:11336/15359instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:48.109CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
Production of Human epidermal growth factor (hegf) nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
title Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
spellingShingle Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
Parsons, J.
Human Epidermal Growth Factor;
Nicotiana Tabacum
Suspension Cultures
Hairy Roots
title_short Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
title_full Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
title_fullStr Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
title_full_unstemmed Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
title_sort Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition
dc.creator.none.fl_str_mv Parsons, J.
Wirth, Sonia Alejandra
Dominguez, M.
Bravo Almonacid, Fernando Felix
Giulietti, Ana Maria
Rodriguez Talou, Julian
author Parsons, J.
author_facet Parsons, J.
Wirth, Sonia Alejandra
Dominguez, M.
Bravo Almonacid, Fernando Felix
Giulietti, Ana Maria
Rodriguez Talou, Julian
author_role author
author2 Wirth, Sonia Alejandra
Dominguez, M.
Bravo Almonacid, Fernando Felix
Giulietti, Ana Maria
Rodriguez Talou, Julian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Human Epidermal Growth Factor;
Nicotiana Tabacum
Suspension Cultures
Hairy Roots
topic Human Epidermal Growth Factor;
Nicotiana Tabacum
Suspension Cultures
Hairy Roots
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The aim of this work was to study the production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum. Suspension and hairy root cultures expressing hEGF were established and the time course of growth and hEGF production were evaluated. In hairy root cultures, hEGF content was about 2 µg g -1FW, with a productivity of 15.9 µg l-1d-1 whereas in cell suspensions, a specific production of 0.48-0.53 µg g -1FW and a productivity of 31 µg l-1 d-1 were obtained. From these results, it is clear that hEGF synthesis and accumulation depend on tissue differentiation. In order to increase hEGF production, cell suspension cultures were treated with casaminoacids and sodium nitroprusside (SNP, a nitric oxide donor). SNP treatment resulted in an increase of hEGF productivity of 48% compared with control cultures. In conclusion, cell suspension and hairy root cultures established from transgenic N. tabacum were able to produce hEGF and the concentration and yield obtained depended on the level of tissue differentiation and the treatment applied.
Fil: Parsons, J.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Dominguez, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina
Fil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Giulietti, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez Talou, Julian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this work was to study the production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum. Suspension and hairy root cultures expressing hEGF were established and the time course of growth and hEGF production were evaluated. In hairy root cultures, hEGF content was about 2 µg g -1FW, with a productivity of 15.9 µg l-1d-1 whereas in cell suspensions, a specific production of 0.48-0.53 µg g -1FW and a productivity of 31 µg l-1 d-1 were obtained. From these results, it is clear that hEGF synthesis and accumulation depend on tissue differentiation. In order to increase hEGF production, cell suspension cultures were treated with casaminoacids and sodium nitroprusside (SNP, a nitric oxide donor). SNP treatment resulted in an increase of hEGF productivity of 48% compared with control cultures. In conclusion, cell suspension and hairy root cultures established from transgenic N. tabacum were able to produce hEGF and the concentration and yield obtained depended on the level of tissue differentiation and the treatment applied.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15359
Parsons, J.; Wirth, Sonia Alejandra; Dominguez, M.; Bravo Almonacid, Fernando Felix; Giulietti, Ana Maria; et al.; Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition; Research India; International Journal of Biotechnology and Biochemistry; 6; 1; 12-2010; 131-138
0973-2691
0974-4762
url http://hdl.handle.net/11336/15359
identifier_str_mv Parsons, J.; Wirth, Sonia Alejandra; Dominguez, M.; Bravo Almonacid, Fernando Felix; Giulietti, Ana Maria; et al.; Production of human epidermal growth factor (hEGF) by in vitro cultures of Nicotiana tabacum: effect of tissue differentiation and sodium nitroprusside addition; Research India; International Journal of Biotechnology and Biochemistry; 6; 1; 12-2010; 131-138
0973-2691
0974-4762
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ripublication.com/Volume/ijbbv6n1.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research India
publisher.none.fl_str_mv Research India
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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