Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation

Autores
Baier, Carlos Javier; Franco, D. L.; Gallegos, Cristina Eugenia; Mongiat, L.; Caviedes, P.; Barrantes, Francisco Jose
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In previous work we determined that prenatal stress affects the expression of α7 nicotinic acetylcholine receptor (α7-AChR) in the frontal cortex of adult rat offspring. The aim of the present study was to investigate whether corticosterone (CORT) affects the biology of AChRs. A neuronal cell line (CNh) derived from cerebral cortex and exhibiting a cholinergic phenotype was treated with CORT (1μM). Whole-cell voltage-clamp recordings showed functional AChR expression at the plasma membrane. Upon treatment with CORT, CNh cells expressed lower levels of α7- AChR (~60% of that in control cells). As a control, α4-AChR was found not to be affected. Morphometric analyses showed that CORT delayed the acquisition of the mature CNh cell phenotype. After 48 h in differentiation medium, cell cycle analysis using flow cytometry showed that control CNh cells were arrested in the G0/ G1 phase (~65-70%), whereas cells grown in the presence of CORT remained undifferentiated (G0/G1<60%). Nicotine treatment affected the differentiation of CNh cells and exerted a synergistic effect together with CORT (G0/G1~40%, G2/M~55-60%). Transfection of CNh cells with GFP-tagged α7-AChR abolished the alterations in the cell cycle in CORT-treated cells. Thus, α7- AChR could act as a modulator of the differentiation of CNh cells and CORT, through this receptor, could impair the acquisition of a mature phenotype.
Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Franco, D. L.. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Gallegos, Cristina Eugenia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mongiat, L.. Fundación Instituto Leloir; Argentina
Fil: Caviedes, P.. Universidad de Chile; Chile
Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
CORTICOSTERONE
ACETYLCHOLINE RECEPTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232375
Acceder
id CONICETDig_924ccb68430cf31882afeeb7cef92a3d
oai_identifier_str oai:ri.conicet.gov.ar:11336/232375
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulationBaier, Carlos JavierFranco, D. L.Gallegos, Cristina EugeniaMongiat, L.Caviedes, P.Barrantes, Francisco JoseCORTICOSTERONEACETYLCHOLINE RECEPTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In previous work we determined that prenatal stress affects the expression of α7 nicotinic acetylcholine receptor (α7-AChR) in the frontal cortex of adult rat offspring. The aim of the present study was to investigate whether corticosterone (CORT) affects the biology of AChRs. A neuronal cell line (CNh) derived from cerebral cortex and exhibiting a cholinergic phenotype was treated with CORT (1μM). Whole-cell voltage-clamp recordings showed functional AChR expression at the plasma membrane. Upon treatment with CORT, CNh cells expressed lower levels of α7- AChR (~60% of that in control cells). As a control, α4-AChR was found not to be affected. Morphometric analyses showed that CORT delayed the acquisition of the mature CNh cell phenotype. After 48 h in differentiation medium, cell cycle analysis using flow cytometry showed that control CNh cells were arrested in the G0/ G1 phase (~65-70%), whereas cells grown in the presence of CORT remained undifferentiated (G0/G1<60%). Nicotine treatment affected the differentiation of CNh cells and exerted a synergistic effect together with CORT (G0/G1~40%, G2/M~55-60%). Transfection of CNh cells with GFP-tagged α7-AChR abolished the alterations in the cell cycle in CORT-treated cells. Thus, α7- AChR could act as a modulator of the differentiation of CNh cells and CORT, through this receptor, could impair the acquisition of a mature phenotype.Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Franco, D. L.. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Gallegos, Cristina Eugenia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mongiat, L.. Fundación Instituto Leloir; ArgentinaFil: Caviedes, P.. Universidad de Chile; ChileFil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaXLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularBiocell2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/232375Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation; XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Argentina; 2013; 19-190327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-37.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:58:37Zoai:ri.conicet.gov.ar:11336/232375instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:58:37.985CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
title Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
spellingShingle Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
Baier, Carlos Javier
CORTICOSTERONE
ACETYLCHOLINE RECEPTOR
title_short Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
title_full Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
title_fullStr Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
title_full_unstemmed Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
title_sort Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation
dc.creator.none.fl_str_mv Baier, Carlos Javier
Franco, D. L.
Gallegos, Cristina Eugenia
Mongiat, L.
Caviedes, P.
Barrantes, Francisco Jose
author Baier, Carlos Javier
author_facet Baier, Carlos Javier
Franco, D. L.
Gallegos, Cristina Eugenia
Mongiat, L.
Caviedes, P.
Barrantes, Francisco Jose
author_role author
author2 Franco, D. L.
Gallegos, Cristina Eugenia
Mongiat, L.
Caviedes, P.
Barrantes, Francisco Jose
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CORTICOSTERONE
ACETYLCHOLINE RECEPTOR
topic CORTICOSTERONE
ACETYLCHOLINE RECEPTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In previous work we determined that prenatal stress affects the expression of α7 nicotinic acetylcholine receptor (α7-AChR) in the frontal cortex of adult rat offspring. The aim of the present study was to investigate whether corticosterone (CORT) affects the biology of AChRs. A neuronal cell line (CNh) derived from cerebral cortex and exhibiting a cholinergic phenotype was treated with CORT (1μM). Whole-cell voltage-clamp recordings showed functional AChR expression at the plasma membrane. Upon treatment with CORT, CNh cells expressed lower levels of α7- AChR (~60% of that in control cells). As a control, α4-AChR was found not to be affected. Morphometric analyses showed that CORT delayed the acquisition of the mature CNh cell phenotype. After 48 h in differentiation medium, cell cycle analysis using flow cytometry showed that control CNh cells were arrested in the G0/ G1 phase (~65-70%), whereas cells grown in the presence of CORT remained undifferentiated (G0/G1<60%). Nicotine treatment affected the differentiation of CNh cells and exerted a synergistic effect together with CORT (G0/G1~40%, G2/M~55-60%). Transfection of CNh cells with GFP-tagged α7-AChR abolished the alterations in the cell cycle in CORT-treated cells. Thus, α7- AChR could act as a modulator of the differentiation of CNh cells and CORT, through this receptor, could impair the acquisition of a mature phenotype.
Fil: Baier, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Franco, D. L.. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Gallegos, Cristina Eugenia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mongiat, L.. Fundación Instituto Leloir; Argentina
Fil: Caviedes, P.. Universidad de Chile; Chile
Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description In previous work we determined that prenatal stress affects the expression of α7 nicotinic acetylcholine receptor (α7-AChR) in the frontal cortex of adult rat offspring. The aim of the present study was to investigate whether corticosterone (CORT) affects the biology of AChRs. A neuronal cell line (CNh) derived from cerebral cortex and exhibiting a cholinergic phenotype was treated with CORT (1μM). Whole-cell voltage-clamp recordings showed functional AChR expression at the plasma membrane. Upon treatment with CORT, CNh cells expressed lower levels of α7- AChR (~60% of that in control cells). As a control, α4-AChR was found not to be affected. Morphometric analyses showed that CORT delayed the acquisition of the mature CNh cell phenotype. After 48 h in differentiation medium, cell cycle analysis using flow cytometry showed that control CNh cells were arrested in the G0/ G1 phase (~65-70%), whereas cells grown in the presence of CORT remained undifferentiated (G0/G1<60%). Nicotine treatment affected the differentiation of CNh cells and exerted a synergistic effect together with CORT (G0/G1~40%, G2/M~55-60%). Transfection of CNh cells with GFP-tagged α7-AChR abolished the alterations in the cell cycle in CORT-treated cells. Thus, α7- AChR could act as a modulator of the differentiation of CNh cells and CORT, through this receptor, could impair the acquisition of a mature phenotype.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232375
Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation; XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Argentina; 2013; 19-19
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232375
identifier_str_mv Corticosterone halts differentiation of a neuronal cell line via acetylcholine receptor modulation; XLIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Argentina; 2013; 19-19
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-37.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606262504226816
score 13.146683

Publicaciones similares