Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step

Autores
Romero, Marcelo Ricardo; Slenk, Walter; Montero, Felipe; Juárez Data, Roger Martín Noé
Año de publicación
2014
Idioma
inglés
Tipo de recurso
libro
Estado
versión publicada
Descripción
A synthesis of IMAC adsorbent was performed. Agarose matrix was derivatized with epichlorohydrin using different amounts of NaBH4. Furthermore, iminodiacetic acid was immobilized on the modified agarose. Finally, the derivatization efficiency was studied by measuring the epoxide groups and retention of Cu2+. The results indicated that the matrices were modified efficiently and the use of NaBH4 in small amounts is enough to provide a reducing environment to the reaction.
Fil: Romero, Marcelo Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Slenk, Walter. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Montero, Felipe. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Juárez Data, Roger Martín Noé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina
Materia
AGAROSE
CHROMATOGRAPHY
COPPER
NABH4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118000
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118000
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization stepRomero, Marcelo RicardoSlenk, WalterMontero, FelipeJuárez Data, Roger Martín NoéAGAROSECHROMATOGRAPHYCOPPERNABH4https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A synthesis of IMAC adsorbent was performed. Agarose matrix was derivatized with epichlorohydrin using different amounts of NaBH4. Furthermore, iminodiacetic acid was immobilized on the modified agarose. Finally, the derivatization efficiency was studied by measuring the epoxide groups and retention of Cu2+. The results indicated that the matrices were modified efficiently and the use of NaBH4 in small amounts is enough to provide a reducing environment to the reaction.Fil: Romero, Marcelo Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Slenk, Walter. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Montero, Felipe. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Juárez Data, Roger Martín Noé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; ArgentinaGRIN Publishing GmbH2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookinfo:ar-repo/semantics/librohttp://purl.org/coar/resource_type/c_2f33application/pdfapplication/pdfhttp://hdl.handle.net/11336/118000Romero, Marcelo Ricardo; Slenk, Walter; Montero, Felipe; Juárez Data, Roger Martín Noé; Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step; GRIN Publishing GmbH; 1; 2014; 59783656846154CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.grin.com/en/e-book/284397/synthesis-of-chromatography-adsorbent-immobilized-metal-affinity-imacinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:31Zoai:ri.conicet.gov.ar:11336/118000instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:31.998CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
title Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
spellingShingle Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
Romero, Marcelo Ricardo
AGAROSE
CHROMATOGRAPHY
COPPER
NABH4
title_short Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
title_full Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
title_fullStr Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
title_full_unstemmed Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
title_sort Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step
dc.creator.none.fl_str_mv Romero, Marcelo Ricardo
Slenk, Walter
Montero, Felipe
Juárez Data, Roger Martín Noé
author Romero, Marcelo Ricardo
author_facet Romero, Marcelo Ricardo
Slenk, Walter
Montero, Felipe
Juárez Data, Roger Martín Noé
author_role author
author2 Slenk, Walter
Montero, Felipe
Juárez Data, Roger Martín Noé
author2_role author
author
author
dc.subject.none.fl_str_mv AGAROSE
CHROMATOGRAPHY
COPPER
NABH4
topic AGAROSE
CHROMATOGRAPHY
COPPER
NABH4
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A synthesis of IMAC adsorbent was performed. Agarose matrix was derivatized with epichlorohydrin using different amounts of NaBH4. Furthermore, iminodiacetic acid was immobilized on the modified agarose. Finally, the derivatization efficiency was studied by measuring the epoxide groups and retention of Cu2+. The results indicated that the matrices were modified efficiently and the use of NaBH4 in small amounts is enough to provide a reducing environment to the reaction.
Fil: Romero, Marcelo Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Slenk, Walter. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Montero, Felipe. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Juárez Data, Roger Martín Noé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina
description A synthesis of IMAC adsorbent was performed. Agarose matrix was derivatized with epichlorohydrin using different amounts of NaBH4. Furthermore, iminodiacetic acid was immobilized on the modified agarose. Finally, the derivatization efficiency was studied by measuring the epoxide groups and retention of Cu2+. The results indicated that the matrices were modified efficiently and the use of NaBH4 in small amounts is enough to provide a reducing environment to the reaction.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/book
info:ar-repo/semantics/libro
http://purl.org/coar/resource_type/c_2f33
status_str publishedVersion
format book
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118000
Romero, Marcelo Ricardo; Slenk, Walter; Montero, Felipe; Juárez Data, Roger Martín Noé; Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step; GRIN Publishing GmbH; 1; 2014; 5
9783656846154
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118000
identifier_str_mv Romero, Marcelo Ricardo; Slenk, Walter; Montero, Felipe; Juárez Data, Roger Martín Noé; Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step; GRIN Publishing GmbH; 1; 2014; 5
9783656846154
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.grin.com/en/e-book/284397/synthesis-of-chromatography-adsorbent-immobilized-metal-affinity-imac
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv GRIN Publishing GmbH
publisher.none.fl_str_mv GRIN Publishing GmbH
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614128955555840
score 13.070432

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