Macroporous monolithic supports for affinity chromatography

Autores
Arrua, Ruben Dario; Alvarez Igarzabal, Cecilia Ines
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.
Fil: Arrua, Ruben Dario . Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Materia
Affinity Chromatography Affinity Chromatography
Macroporus Materials
Monolithic Supports
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13249
Acceder
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spelling Macroporous monolithic supports for affinity chromatographyArrua, Ruben Dario Alvarez Igarzabal, Cecilia InesAffinity Chromatography Affinity ChromatographyMacroporus MaterialsMonolithic Supportshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.Fil: Arrua, Ruben Dario . Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; ArgentinaFil: Alvarez Igarzabal, Cecilia Ines. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaWiley2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13249Arrua, Ruben Dario ; Alvarez Igarzabal, Cecilia Ines; Macroporous monolithic supports for affinity chromatography; Wiley; Journal Of Separation Science - (online); 34; 16-17; 3-2011; 1974-19871615-9314enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jssc.201100197/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/jssc.201100197info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:34:07Zoai:ri.conicet.gov.ar:11336/13249instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:34:08.085CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Macroporous monolithic supports for affinity chromatography
title Macroporous monolithic supports for affinity chromatography
spellingShingle Macroporous monolithic supports for affinity chromatography
Arrua, Ruben Dario
Affinity Chromatography Affinity Chromatography
Macroporus Materials
Monolithic Supports
title_short Macroporous monolithic supports for affinity chromatography
title_full Macroporous monolithic supports for affinity chromatography
title_fullStr Macroporous monolithic supports for affinity chromatography
title_full_unstemmed Macroporous monolithic supports for affinity chromatography
title_sort Macroporous monolithic supports for affinity chromatography
dc.creator.none.fl_str_mv Arrua, Ruben Dario
Alvarez Igarzabal, Cecilia Ines
author Arrua, Ruben Dario
author_facet Arrua, Ruben Dario
Alvarez Igarzabal, Cecilia Ines
author_role author
author2 Alvarez Igarzabal, Cecilia Ines
author2_role author
dc.subject.none.fl_str_mv Affinity Chromatography Affinity Chromatography
Macroporus Materials
Monolithic Supports
topic Affinity Chromatography Affinity Chromatography
Macroporus Materials
Monolithic Supports
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.
Fil: Arrua, Ruben Dario . Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
description In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13249
Arrua, Ruben Dario ; Alvarez Igarzabal, Cecilia Ines; Macroporous monolithic supports for affinity chromatography; Wiley; Journal Of Separation Science - (online); 34; 16-17; 3-2011; 1974-1987
1615-9314
url http://hdl.handle.net/11336/13249
identifier_str_mv Arrua, Ruben Dario ; Alvarez Igarzabal, Cecilia Ines; Macroporous monolithic supports for affinity chromatography; Wiley; Journal Of Separation Science - (online); 34; 16-17; 3-2011; 1974-1987
1615-9314
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jssc.201100197/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/jssc.201100197
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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