Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10

Autores
Moreno, Javier Edgardo; Shyu, Christine; Campos, Marcelo L.; Patel, Lalita C.; Chung, Hoo Sun; Yao, Jian; He, Sheng Hang; Howe, Gregg A.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The plant hormone jasmonate (JA) activates gene expression by promoting ubiquitin-dependent degradation of JAZ transcriptional repressor proteins. A key feature of all JAZ proteins is the highly conserved Jas motif, which mediates both JAZ degradation and JAZ binding to the transcription factor MYC2. Rapid expression of JAZ genes in response to JA is thought to attenuate JA responses, but little is known about the mechanisms by which newly synthesized JAZ proteins exert repression in the presence of the hormone. Here, we show that desensitization to JA is mediated by an alternative splice variant (JAZ10.4) of JAZ10 that lacks the Jas motif. Unbiased protein-protein interaction screens identified three related bHLH transcription factors (MYC2, MYC3, and MYC4) and the co-repressor NINJA as JAZ10.4-binding partners. We show that the N-terminal region of JAZ10.4 contains a cryptic MYC2-binding site that resembles the Jas motif, and that the ZIM motif of JAZ10.4 functions as a transferable repressor domain whose activity is associated with recruitment of NINJA. Functional studies showed that expression of JAZ10.4 from the native JAZ10 promoter complemented the JA-hypersensitive phenotype of a jaz10 mutant. Moreover, treatment of these complemented lines with JA resulted in rapid accumulation of JAZ10.4 protein. Our results provide an explanation for how the unique domain architecture of JAZ10.4 links transcription factors to a co-repressor complex, and suggest how JA-induced transcription and alternative splicing of JAZ10 pre-mRNA creates a regulatory circuit to attenuate JA responses.
Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Michigan State University; Estados Unidos
Fil: Shyu, Christine. Michigan State University; Estados Unidos
Fil: Campos, Marcelo L.. Michigan State University; Estados Unidos
Fil: Patel, Lalita C.. Michigan State University; Estados Unidos
Fil: Chung, Hoo Sun. Michigan State University; Estados Unidos
Fil: Yao, Jian. Michigan State University; Estados Unidos
Fil: He, Sheng Hang. Michigan State University; Estados Unidos
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
Materia
JAZ10
SPLICING
MYC2
CRYPTIC_DOMAIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23466
Acceder
id CONICETDig_91762e96756d4d55ff073296efeec995
oai_identifier_str oai:ri.conicet.gov.ar:11336/23466
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10Moreno, Javier EdgardoShyu, ChristineCampos, Marcelo L.Patel, Lalita C.Chung, Hoo SunYao, JianHe, Sheng HangHowe, Gregg A.JAZ10SPLICINGMYC2CRYPTIC_DOMAINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The plant hormone jasmonate (JA) activates gene expression by promoting ubiquitin-dependent degradation of JAZ transcriptional repressor proteins. A key feature of all JAZ proteins is the highly conserved Jas motif, which mediates both JAZ degradation and JAZ binding to the transcription factor MYC2. Rapid expression of JAZ genes in response to JA is thought to attenuate JA responses, but little is known about the mechanisms by which newly synthesized JAZ proteins exert repression in the presence of the hormone. Here, we show that desensitization to JA is mediated by an alternative splice variant (JAZ10.4) of JAZ10 that lacks the Jas motif. Unbiased protein-protein interaction screens identified three related bHLH transcription factors (MYC2, MYC3, and MYC4) and the co-repressor NINJA as JAZ10.4-binding partners. We show that the N-terminal region of JAZ10.4 contains a cryptic MYC2-binding site that resembles the Jas motif, and that the ZIM motif of JAZ10.4 functions as a transferable repressor domain whose activity is associated with recruitment of NINJA. Functional studies showed that expression of JAZ10.4 from the native JAZ10 promoter complemented the JA-hypersensitive phenotype of a jaz10 mutant. Moreover, treatment of these complemented lines with JA resulted in rapid accumulation of JAZ10.4 protein. Our results provide an explanation for how the unique domain architecture of JAZ10.4 links transcription factors to a co-repressor complex, and suggest how JA-induced transcription and alternative splicing of JAZ10 pre-mRNA creates a regulatory circuit to attenuate JA responses.Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Michigan State University; Estados UnidosFil: Shyu, Christine. Michigan State University; Estados UnidosFil: Campos, Marcelo L.. Michigan State University; Estados UnidosFil: Patel, Lalita C.. Michigan State University; Estados UnidosFil: Chung, Hoo Sun. Michigan State University; Estados UnidosFil: Yao, Jian. Michigan State University; Estados UnidosFil: He, Sheng Hang. Michigan State University; Estados UnidosFil: Howe, Gregg A.. Michigan State University; Estados UnidosAmerican Society of Plant Biologist2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23466Moreno, Javier Edgardo; Shyu, Christine; Campos, Marcelo L.; Patel, Lalita C.; Chung, Hoo Sun; et al.; Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10; American Society of Plant Biologist; Plant Physiology; 162; 3; 4-2013; 1006-10170032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/162/3/1434info:eu-repo/semantics/altIdentifier/doi/10.1104%2Fpp.113.216416info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:36:38Zoai:ri.conicet.gov.ar:11336/23466instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:36:38.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
title Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
spellingShingle Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
Moreno, Javier Edgardo
JAZ10
SPLICING
MYC2
CRYPTIC_DOMAIN
title_short Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
title_full Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
title_fullStr Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
title_full_unstemmed Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
title_sort Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10
dc.creator.none.fl_str_mv Moreno, Javier Edgardo
Shyu, Christine
Campos, Marcelo L.
Patel, Lalita C.
Chung, Hoo Sun
Yao, Jian
He, Sheng Hang
Howe, Gregg A.
author Moreno, Javier Edgardo
author_facet Moreno, Javier Edgardo
Shyu, Christine
Campos, Marcelo L.
Patel, Lalita C.
Chung, Hoo Sun
Yao, Jian
He, Sheng Hang
Howe, Gregg A.
author_role author
author2 Shyu, Christine
Campos, Marcelo L.
Patel, Lalita C.
Chung, Hoo Sun
Yao, Jian
He, Sheng Hang
Howe, Gregg A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv JAZ10
SPLICING
MYC2
CRYPTIC_DOMAIN
topic JAZ10
SPLICING
MYC2
CRYPTIC_DOMAIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The plant hormone jasmonate (JA) activates gene expression by promoting ubiquitin-dependent degradation of JAZ transcriptional repressor proteins. A key feature of all JAZ proteins is the highly conserved Jas motif, which mediates both JAZ degradation and JAZ binding to the transcription factor MYC2. Rapid expression of JAZ genes in response to JA is thought to attenuate JA responses, but little is known about the mechanisms by which newly synthesized JAZ proteins exert repression in the presence of the hormone. Here, we show that desensitization to JA is mediated by an alternative splice variant (JAZ10.4) of JAZ10 that lacks the Jas motif. Unbiased protein-protein interaction screens identified three related bHLH transcription factors (MYC2, MYC3, and MYC4) and the co-repressor NINJA as JAZ10.4-binding partners. We show that the N-terminal region of JAZ10.4 contains a cryptic MYC2-binding site that resembles the Jas motif, and that the ZIM motif of JAZ10.4 functions as a transferable repressor domain whose activity is associated with recruitment of NINJA. Functional studies showed that expression of JAZ10.4 from the native JAZ10 promoter complemented the JA-hypersensitive phenotype of a jaz10 mutant. Moreover, treatment of these complemented lines with JA resulted in rapid accumulation of JAZ10.4 protein. Our results provide an explanation for how the unique domain architecture of JAZ10.4 links transcription factors to a co-repressor complex, and suggest how JA-induced transcription and alternative splicing of JAZ10 pre-mRNA creates a regulatory circuit to attenuate JA responses.
Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Michigan State University; Estados Unidos
Fil: Shyu, Christine. Michigan State University; Estados Unidos
Fil: Campos, Marcelo L.. Michigan State University; Estados Unidos
Fil: Patel, Lalita C.. Michigan State University; Estados Unidos
Fil: Chung, Hoo Sun. Michigan State University; Estados Unidos
Fil: Yao, Jian. Michigan State University; Estados Unidos
Fil: He, Sheng Hang. Michigan State University; Estados Unidos
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
description The plant hormone jasmonate (JA) activates gene expression by promoting ubiquitin-dependent degradation of JAZ transcriptional repressor proteins. A key feature of all JAZ proteins is the highly conserved Jas motif, which mediates both JAZ degradation and JAZ binding to the transcription factor MYC2. Rapid expression of JAZ genes in response to JA is thought to attenuate JA responses, but little is known about the mechanisms by which newly synthesized JAZ proteins exert repression in the presence of the hormone. Here, we show that desensitization to JA is mediated by an alternative splice variant (JAZ10.4) of JAZ10 that lacks the Jas motif. Unbiased protein-protein interaction screens identified three related bHLH transcription factors (MYC2, MYC3, and MYC4) and the co-repressor NINJA as JAZ10.4-binding partners. We show that the N-terminal region of JAZ10.4 contains a cryptic MYC2-binding site that resembles the Jas motif, and that the ZIM motif of JAZ10.4 functions as a transferable repressor domain whose activity is associated with recruitment of NINJA. Functional studies showed that expression of JAZ10.4 from the native JAZ10 promoter complemented the JA-hypersensitive phenotype of a jaz10 mutant. Moreover, treatment of these complemented lines with JA resulted in rapid accumulation of JAZ10.4 protein. Our results provide an explanation for how the unique domain architecture of JAZ10.4 links transcription factors to a co-repressor complex, and suggest how JA-induced transcription and alternative splicing of JAZ10 pre-mRNA creates a regulatory circuit to attenuate JA responses.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23466
Moreno, Javier Edgardo; Shyu, Christine; Campos, Marcelo L.; Patel, Lalita C.; Chung, Hoo Sun; et al.; Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10; American Society of Plant Biologist; Plant Physiology; 162; 3; 4-2013; 1006-1017
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23466
identifier_str_mv Moreno, Javier Edgardo; Shyu, Christine; Campos, Marcelo L.; Patel, Lalita C.; Chung, Hoo Sun; et al.; Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10; American Society of Plant Biologist; Plant Physiology; 162; 3; 4-2013; 1006-1017
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/162/3/1434
info:eu-repo/semantics/altIdentifier/doi/10.1104%2Fpp.113.216416
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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