BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses

Autores
Peppino Margutti, Micaela Yesica; Alvarez, Maria Elena; Cecchini, Nicolas Miguel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Plants depend on a non-adaptive immune system triggered by receptors after the detection of pathogens. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key sensors that recognize specific effectors (or effector-induced alterations) used by pathogens to promote their virulence. Several studies indicate that NLRs can exert their function at the nucleus, plasma membrane, cytoplasm, or the endomembrane system. However, NLRs acting from plastids have never been described. Interestingly, results from our group showed that the BNT1 immune receptor from Arabidopsis is targeted to plastids envelope. Thus, activation of this NLR could modulate organelle-specific responses required for a normal defense, like the production of defense-related metabolites and/or induction of plastid-nucleus retrograde communication. Supporting this idea, our preliminary results showed that overexpression of BNT1 mutated versions induces plastids to cluster around nuclei. Here, we analyzed BNT1 expression profiles in different developmental stages, stress conditions, and/or chemical treatments. Analysis of RNA-seq databases showed that BNT1 transcript levels change in seedlings grown under light or dark conditions. Moreover, it also revealed three different BNT1 isoforms generated by alternative splicing (AS) in the gene 5’ UTR. Our RT-PCR studies validated the presence of these splice variants and indicated that their relative abundance is affected by known splicing factors. We also observed expression differences under heat-stress and in response to exogenous treatments with immunity inducers, hormones, and methyl viologen. Curiously, only one of the BNT1 isoforms exhibits a complete plastid targeting signal. This strongly suggests that AS at the 5’ UTR can control the BNT1 subcellular localization and site of action. The elucidation of BNT1 biological role and its regulation by AS could represent a new paradigm to understand the function of NLRs at different cellular compartments.
Fil: Peppino Margutti, Micaela Yesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology y XV Annual Meeting Argentinean Society for General Microbiology
Virtual
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
BNT1
R proteins
Splicing
Signal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271829
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/271829
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responsesPeppino Margutti, Micaela YesicaAlvarez, Maria ElenaCecchini, Nicolas MiguelBNT1R proteinsSplicingSignalhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plants depend on a non-adaptive immune system triggered by receptors after the detection of pathogens. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key sensors that recognize specific effectors (or effector-induced alterations) used by pathogens to promote their virulence. Several studies indicate that NLRs can exert their function at the nucleus, plasma membrane, cytoplasm, or the endomembrane system. However, NLRs acting from plastids have never been described. Interestingly, results from our group showed that the BNT1 immune receptor from Arabidopsis is targeted to plastids envelope. Thus, activation of this NLR could modulate organelle-specific responses required for a normal defense, like the production of defense-related metabolites and/or induction of plastid-nucleus retrograde communication. Supporting this idea, our preliminary results showed that overexpression of BNT1 mutated versions induces plastids to cluster around nuclei. Here, we analyzed BNT1 expression profiles in different developmental stages, stress conditions, and/or chemical treatments. Analysis of RNA-seq databases showed that BNT1 transcript levels change in seedlings grown under light or dark conditions. Moreover, it also revealed three different BNT1 isoforms generated by alternative splicing (AS) in the gene 5’ UTR. Our RT-PCR studies validated the presence of these splice variants and indicated that their relative abundance is affected by known splicing factors. We also observed expression differences under heat-stress and in response to exogenous treatments with immunity inducers, hormones, and methyl viologen. Curiously, only one of the BNT1 isoforms exhibits a complete plastid targeting signal. This strongly suggests that AS at the 5’ UTR can control the BNT1 subcellular localization and site of action. The elucidation of BNT1 biological role and its regulation by AS could represent a new paradigm to understand the function of NLRs at different cellular compartments.Fil: Peppino Margutti, Micaela Yesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology y XV Annual Meeting Argentinean Society for General MicrobiologyVirtualArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/271829BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology y XV Annual Meeting Argentinean Society for General Microbiology; Virtual; Argentina; 2020; 72-720327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/libros/2020.pdfinfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/congresos-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:43Zoai:ri.conicet.gov.ar:11336/271829instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:43.829CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
title BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
spellingShingle BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
Peppino Margutti, Micaela Yesica
BNT1
R proteins
Splicing
Signal
title_short BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
title_full BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
title_fullStr BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
title_full_unstemmed BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
title_sort BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses
dc.creator.none.fl_str_mv Peppino Margutti, Micaela Yesica
Alvarez, Maria Elena
Cecchini, Nicolas Miguel
author Peppino Margutti, Micaela Yesica
author_facet Peppino Margutti, Micaela Yesica
Alvarez, Maria Elena
Cecchini, Nicolas Miguel
author_role author
author2 Alvarez, Maria Elena
Cecchini, Nicolas Miguel
author2_role author
author
dc.subject.none.fl_str_mv BNT1
R proteins
Splicing
Signal
topic BNT1
R proteins
Splicing
Signal
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plants depend on a non-adaptive immune system triggered by receptors after the detection of pathogens. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key sensors that recognize specific effectors (or effector-induced alterations) used by pathogens to promote their virulence. Several studies indicate that NLRs can exert their function at the nucleus, plasma membrane, cytoplasm, or the endomembrane system. However, NLRs acting from plastids have never been described. Interestingly, results from our group showed that the BNT1 immune receptor from Arabidopsis is targeted to plastids envelope. Thus, activation of this NLR could modulate organelle-specific responses required for a normal defense, like the production of defense-related metabolites and/or induction of plastid-nucleus retrograde communication. Supporting this idea, our preliminary results showed that overexpression of BNT1 mutated versions induces plastids to cluster around nuclei. Here, we analyzed BNT1 expression profiles in different developmental stages, stress conditions, and/or chemical treatments. Analysis of RNA-seq databases showed that BNT1 transcript levels change in seedlings grown under light or dark conditions. Moreover, it also revealed three different BNT1 isoforms generated by alternative splicing (AS) in the gene 5’ UTR. Our RT-PCR studies validated the presence of these splice variants and indicated that their relative abundance is affected by known splicing factors. We also observed expression differences under heat-stress and in response to exogenous treatments with immunity inducers, hormones, and methyl viologen. Curiously, only one of the BNT1 isoforms exhibits a complete plastid targeting signal. This strongly suggests that AS at the 5’ UTR can control the BNT1 subcellular localization and site of action. The elucidation of BNT1 biological role and its regulation by AS could represent a new paradigm to understand the function of NLRs at different cellular compartments.
Fil: Peppino Margutti, Micaela Yesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology y XV Annual Meeting Argentinean Society for General Microbiology
Virtual
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description Plants depend on a non-adaptive immune system triggered by receptors after the detection of pathogens. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key sensors that recognize specific effectors (or effector-induced alterations) used by pathogens to promote their virulence. Several studies indicate that NLRs can exert their function at the nucleus, plasma membrane, cytoplasm, or the endomembrane system. However, NLRs acting from plastids have never been described. Interestingly, results from our group showed that the BNT1 immune receptor from Arabidopsis is targeted to plastids envelope. Thus, activation of this NLR could modulate organelle-specific responses required for a normal defense, like the production of defense-related metabolites and/or induction of plastid-nucleus retrograde communication. Supporting this idea, our preliminary results showed that overexpression of BNT1 mutated versions induces plastids to cluster around nuclei. Here, we analyzed BNT1 expression profiles in different developmental stages, stress conditions, and/or chemical treatments. Analysis of RNA-seq databases showed that BNT1 transcript levels change in seedlings grown under light or dark conditions. Moreover, it also revealed three different BNT1 isoforms generated by alternative splicing (AS) in the gene 5’ UTR. Our RT-PCR studies validated the presence of these splice variants and indicated that their relative abundance is affected by known splicing factors. We also observed expression differences under heat-stress and in response to exogenous treatments with immunity inducers, hormones, and methyl viologen. Curiously, only one of the BNT1 isoforms exhibits a complete plastid targeting signal. This strongly suggests that AS at the 5’ UTR can control the BNT1 subcellular localization and site of action. The elucidation of BNT1 biological role and its regulation by AS could represent a new paradigm to understand the function of NLRs at different cellular compartments.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271829
BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology y XV Annual Meeting Argentinean Society for General Microbiology; Virtual; Argentina; 2020; 72-72
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271829
identifier_str_mv BNT1 immune receptor alternative spliced variants: Potential role(s) in plastid responses; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology y XV Annual Meeting Argentinean Society for General Microbiology; Virtual; Argentina; 2020; 72-72
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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