Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling
- Autores
- Zhou, X.Edward; Zhang, Yaguang; Yao, Jian; Zheng, Jie; Zhou, Yuxin; He, Qing; Moreno, Javier Edgardo; Lam, Vinh Q.; Cao, Xiaoman; Sugimoto, Kiochi; Vanegas Cano, Leidy; Kariapper, Leena; Suino Powell, Kelly; Zhu, Yuanye; Novick, Scott; Griffin, Patrick R.; Zhang, Feng; Howe, Gregg A.; Melcher, Karsten
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Jasmonates (JAs) are plant hormones with crucial roles in development and stress37 resilience. They activate MYC transcription factors by mediating the proteolysis of MYC38 inhibitors called JAZ. In the absence of JA, JAZ proteins bind and inhibit MYC through39 the assembly of MYC–JAZ–NINJA–TPL repressor complexes. However, JAZ and NINJA40 are predicted to be largely intrinsically unstructured, which precluded their experimental41 structure determination. Through a combination of biochemical, mutational, and42 biophysical analyses and AlphaFold-derived ColabFold modeling, we characterized JAZ–43 JAZ and JAZ–NINJA interactions and generated models with detailed, high confidence44 domain interfaces. We demonstrate that JAZ, NINJA, and MYC interface domains are45 dynamic in isolation and become stabilized in a stepwise order upon complex assembly. In46 contrast, most JAZ and NINJA regions outside of the interfaces remain highly dynamic47 and cannot be modeled in a single conformation. Our data indicate that the small JAZ ZIM48 motif mediates JAZ–JAZ and JAZ–NINJA interactions through separate surfaces, and49 further suggest that NINJA modulates JAZ dimerization. This study advances knowledge50 of JA signaling by providing insight into dynamics, interactions, and structure of the JAZ–51 NINJA core of the JA repressor complex.
Fil: Zhou, X.Edward. Michigan State University; Estados Unidos
Fil: Zhang, Yaguang. Michigan State University; Estados Unidos
Fil: Yao, Jian. Michigan State University; Estados Unidos
Fil: Zheng, Jie. Michigan State University; Estados Unidos
Fil: Zhou, Yuxin. Michigan State University; Estados Unidos
Fil: He, Qing. Michigan State University; Estados Unidos
Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Lam, Vinh Q.. Michigan State University; Estados Unidos
Fil: Cao, Xiaoman. Michigan State University; Estados Unidos
Fil: Sugimoto, Kiochi. Michigan State University; Estados Unidos
Fil: Vanegas Cano, Leidy. Michigan State University; Estados Unidos
Fil: Kariapper, Leena. Michigan State University; Estados Unidos
Fil: Suino Powell, Kelly. Michigan State University; Estados Unidos
Fil: Zhu, Yuanye. Michigan State University; Estados Unidos
Fil: Novick, Scott. Michigan State University; Estados Unidos
Fil: Griffin, Patrick R.. Michigan State University; Estados Unidos
Fil: Zhang, Feng. Michigan State University; Estados Unidos
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
Fil: Melcher, Karsten. Michigan State University; Estados Unidos - Materia
-
JAS
NINJA
ALPHAFOLD
CAOLBAFOLD - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/240877
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signalingZhou, X.EdwardZhang, YaguangYao, JianZheng, JieZhou, YuxinHe, QingMoreno, Javier EdgardoLam, Vinh Q.Cao, XiaomanSugimoto, KiochiVanegas Cano, LeidyKariapper, LeenaSuino Powell, KellyZhu, YuanyeNovick, ScottGriffin, Patrick R.Zhang, FengHowe, Gregg A.Melcher, KarstenJASNINJAALPHAFOLDCAOLBAFOLDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Jasmonates (JAs) are plant hormones with crucial roles in development and stress37 resilience. They activate MYC transcription factors by mediating the proteolysis of MYC38 inhibitors called JAZ. In the absence of JA, JAZ proteins bind and inhibit MYC through39 the assembly of MYC–JAZ–NINJA–TPL repressor complexes. However, JAZ and NINJA40 are predicted to be largely intrinsically unstructured, which precluded their experimental41 structure determination. Through a combination of biochemical, mutational, and42 biophysical analyses and AlphaFold-derived ColabFold modeling, we characterized JAZ–43 JAZ and JAZ–NINJA interactions and generated models with detailed, high confidence44 domain interfaces. We demonstrate that JAZ, NINJA, and MYC interface domains are45 dynamic in isolation and become stabilized in a stepwise order upon complex assembly. In46 contrast, most JAZ and NINJA regions outside of the interfaces remain highly dynamic47 and cannot be modeled in a single conformation. Our data indicate that the small JAZ ZIM48 motif mediates JAZ–JAZ and JAZ–NINJA interactions through separate surfaces, and49 further suggest that NINJA modulates JAZ dimerization. This study advances knowledge50 of JA signaling by providing insight into dynamics, interactions, and structure of the JAZ–51 NINJA core of the JA repressor complex.Fil: Zhou, X.Edward. Michigan State University; Estados UnidosFil: Zhang, Yaguang. Michigan State University; Estados UnidosFil: Yao, Jian. Michigan State University; Estados UnidosFil: Zheng, Jie. Michigan State University; Estados UnidosFil: Zhou, Yuxin. Michigan State University; Estados UnidosFil: He, Qing. Michigan State University; Estados UnidosFil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Lam, Vinh Q.. Michigan State University; Estados UnidosFil: Cao, Xiaoman. Michigan State University; Estados UnidosFil: Sugimoto, Kiochi. Michigan State University; Estados UnidosFil: Vanegas Cano, Leidy. Michigan State University; Estados UnidosFil: Kariapper, Leena. Michigan State University; Estados UnidosFil: Suino Powell, Kelly. Michigan State University; Estados UnidosFil: Zhu, Yuanye. Michigan State University; Estados UnidosFil: Novick, Scott. Michigan State University; Estados UnidosFil: Griffin, Patrick R.. Michigan State University; Estados UnidosFil: Zhang, Feng. Michigan State University; Estados UnidosFil: Howe, Gregg A.. Michigan State University; Estados UnidosFil: Melcher, Karsten. Michigan State University; Estados UnidosCell Press2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240877Zhou, X.Edward; Zhang, Yaguang; Yao, Jian; Zheng, Jie; Zhou, Yuxin; et al.; Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling; Cell Press; Plant Communications; 4; 6; 6-2023; 1-352590-3462CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2590346223001566info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xplc.2023.100639info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:54:32Zoai:ri.conicet.gov.ar:11336/240877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:54:32.256CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| title |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| spellingShingle |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling Zhou, X.Edward JAS NINJA ALPHAFOLD CAOLBAFOLD |
| title_short |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| title_full |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| title_fullStr |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| title_full_unstemmed |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| title_sort |
Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling |
| dc.creator.none.fl_str_mv |
Zhou, X.Edward Zhang, Yaguang Yao, Jian Zheng, Jie Zhou, Yuxin He, Qing Moreno, Javier Edgardo Lam, Vinh Q. Cao, Xiaoman Sugimoto, Kiochi Vanegas Cano, Leidy Kariapper, Leena Suino Powell, Kelly Zhu, Yuanye Novick, Scott Griffin, Patrick R. Zhang, Feng Howe, Gregg A. Melcher, Karsten |
| author |
Zhou, X.Edward |
| author_facet |
Zhou, X.Edward Zhang, Yaguang Yao, Jian Zheng, Jie Zhou, Yuxin He, Qing Moreno, Javier Edgardo Lam, Vinh Q. Cao, Xiaoman Sugimoto, Kiochi Vanegas Cano, Leidy Kariapper, Leena Suino Powell, Kelly Zhu, Yuanye Novick, Scott Griffin, Patrick R. Zhang, Feng Howe, Gregg A. Melcher, Karsten |
| author_role |
author |
| author2 |
Zhang, Yaguang Yao, Jian Zheng, Jie Zhou, Yuxin He, Qing Moreno, Javier Edgardo Lam, Vinh Q. Cao, Xiaoman Sugimoto, Kiochi Vanegas Cano, Leidy Kariapper, Leena Suino Powell, Kelly Zhu, Yuanye Novick, Scott Griffin, Patrick R. Zhang, Feng Howe, Gregg A. Melcher, Karsten |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
JAS NINJA ALPHAFOLD CAOLBAFOLD |
| topic |
JAS NINJA ALPHAFOLD CAOLBAFOLD |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Jasmonates (JAs) are plant hormones with crucial roles in development and stress37 resilience. They activate MYC transcription factors by mediating the proteolysis of MYC38 inhibitors called JAZ. In the absence of JA, JAZ proteins bind and inhibit MYC through39 the assembly of MYC–JAZ–NINJA–TPL repressor complexes. However, JAZ and NINJA40 are predicted to be largely intrinsically unstructured, which precluded their experimental41 structure determination. Through a combination of biochemical, mutational, and42 biophysical analyses and AlphaFold-derived ColabFold modeling, we characterized JAZ–43 JAZ and JAZ–NINJA interactions and generated models with detailed, high confidence44 domain interfaces. We demonstrate that JAZ, NINJA, and MYC interface domains are45 dynamic in isolation and become stabilized in a stepwise order upon complex assembly. In46 contrast, most JAZ and NINJA regions outside of the interfaces remain highly dynamic47 and cannot be modeled in a single conformation. Our data indicate that the small JAZ ZIM48 motif mediates JAZ–JAZ and JAZ–NINJA interactions through separate surfaces, and49 further suggest that NINJA modulates JAZ dimerization. This study advances knowledge50 of JA signaling by providing insight into dynamics, interactions, and structure of the JAZ–51 NINJA core of the JA repressor complex. Fil: Zhou, X.Edward. Michigan State University; Estados Unidos Fil: Zhang, Yaguang. Michigan State University; Estados Unidos Fil: Yao, Jian. Michigan State University; Estados Unidos Fil: Zheng, Jie. Michigan State University; Estados Unidos Fil: Zhou, Yuxin. Michigan State University; Estados Unidos Fil: He, Qing. Michigan State University; Estados Unidos Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Lam, Vinh Q.. Michigan State University; Estados Unidos Fil: Cao, Xiaoman. Michigan State University; Estados Unidos Fil: Sugimoto, Kiochi. Michigan State University; Estados Unidos Fil: Vanegas Cano, Leidy. Michigan State University; Estados Unidos Fil: Kariapper, Leena. Michigan State University; Estados Unidos Fil: Suino Powell, Kelly. Michigan State University; Estados Unidos Fil: Zhu, Yuanye. Michigan State University; Estados Unidos Fil: Novick, Scott. Michigan State University; Estados Unidos Fil: Griffin, Patrick R.. Michigan State University; Estados Unidos Fil: Zhang, Feng. Michigan State University; Estados Unidos Fil: Howe, Gregg A.. Michigan State University; Estados Unidos Fil: Melcher, Karsten. Michigan State University; Estados Unidos |
| description |
Jasmonates (JAs) are plant hormones with crucial roles in development and stress37 resilience. They activate MYC transcription factors by mediating the proteolysis of MYC38 inhibitors called JAZ. In the absence of JA, JAZ proteins bind and inhibit MYC through39 the assembly of MYC–JAZ–NINJA–TPL repressor complexes. However, JAZ and NINJA40 are predicted to be largely intrinsically unstructured, which precluded their experimental41 structure determination. Through a combination of biochemical, mutational, and42 biophysical analyses and AlphaFold-derived ColabFold modeling, we characterized JAZ–43 JAZ and JAZ–NINJA interactions and generated models with detailed, high confidence44 domain interfaces. We demonstrate that JAZ, NINJA, and MYC interface domains are45 dynamic in isolation and become stabilized in a stepwise order upon complex assembly. In46 contrast, most JAZ and NINJA regions outside of the interfaces remain highly dynamic47 and cannot be modeled in a single conformation. Our data indicate that the small JAZ ZIM48 motif mediates JAZ–JAZ and JAZ–NINJA interactions through separate surfaces, and49 further suggest that NINJA modulates JAZ dimerization. This study advances knowledge50 of JA signaling by providing insight into dynamics, interactions, and structure of the JAZ–51 NINJA core of the JA repressor complex. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/240877 Zhou, X.Edward; Zhang, Yaguang; Yao, Jian; Zheng, Jie; Zhou, Yuxin; et al.; Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling; Cell Press; Plant Communications; 4; 6; 6-2023; 1-35 2590-3462 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/240877 |
| identifier_str_mv |
Zhou, X.Edward; Zhang, Yaguang; Yao, Jian; Zheng, Jie; Zhou, Yuxin; et al.; Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling; Cell Press; Plant Communications; 4; 6; 6-2023; 1-35 2590-3462 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2590346223001566 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xplc.2023.100639 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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openAccess |
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application/pdf application/pdf |
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Cell Press |
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Cell Press |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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