Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate

Autores
Gangoiti, Maria Virginia; Anbinder, Pablo Sebastián; Cortizo, Ana Maria; Mccarthy, Antonio Desmond
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with Diabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the in vitro alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5 M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8 M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
Fil: Gangoiti, Maria Virginia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
Fil: Anbinder, Pablo Sebastián. Universidad Nacional de la Plata. Facultad de Ingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cortizo, Ana Maria. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
Fil: Mccarthy, Antonio Desmond. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
Materia
Osteoblast
Advanced Glycation Endproducts
Bisphosphonate
Apoptosis
Actin Cytoskeleton
Environmental Scanning Electron Microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5142
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5142
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronateGangoiti, Maria VirginiaAnbinder, Pablo SebastiánCortizo, Ana MariaMccarthy, Antonio DesmondOsteoblastAdvanced Glycation EndproductsBisphosphonateApoptosisActin CytoskeletonEnvironmental Scanning Electron Microscopyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with Diabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the in vitro alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5 M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8 M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.Fil: Gangoiti, Maria Virginia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; ArgentinaFil: Anbinder, Pablo Sebastián. Universidad Nacional de la Plata. Facultad de Ingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Cortizo, Ana Maria. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; ArgentinaFil: Mccarthy, Antonio Desmond. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; ArgentinaElsevier2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5142Gangoiti, Maria Virginia; Anbinder, Pablo Sebastián; Cortizo, Ana Maria; Mccarthy, Antonio Desmond; Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate; Elsevier; Acta Histochemica; 115; 7; 9-2013; 649-6570065-1281enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0065128113000184info:eu-repo/semantics/altIdentifier/doi/10.1016/j.acthis.2013.01.004info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:37Zoai:ri.conicet.gov.ar:11336/5142instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:37.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
spellingShingle Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
Gangoiti, Maria Virginia
Osteoblast
Advanced Glycation Endproducts
Bisphosphonate
Apoptosis
Actin Cytoskeleton
Environmental Scanning Electron Microscopy
title_short Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_full Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_fullStr Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_full_unstemmed Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_sort Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
dc.creator.none.fl_str_mv Gangoiti, Maria Virginia
Anbinder, Pablo Sebastián
Cortizo, Ana Maria
Mccarthy, Antonio Desmond
author Gangoiti, Maria Virginia
author_facet Gangoiti, Maria Virginia
Anbinder, Pablo Sebastián
Cortizo, Ana Maria
Mccarthy, Antonio Desmond
author_role author
author2 Anbinder, Pablo Sebastián
Cortizo, Ana Maria
Mccarthy, Antonio Desmond
author2_role author
author
author
dc.subject.none.fl_str_mv Osteoblast
Advanced Glycation Endproducts
Bisphosphonate
Apoptosis
Actin Cytoskeleton
Environmental Scanning Electron Microscopy
topic Osteoblast
Advanced Glycation Endproducts
Bisphosphonate
Apoptosis
Actin Cytoskeleton
Environmental Scanning Electron Microscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with Diabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the in vitro alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5 M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8 M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
Fil: Gangoiti, Maria Virginia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
Fil: Anbinder, Pablo Sebastián. Universidad Nacional de la Plata. Facultad de Ingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cortizo, Ana Maria. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
Fil: Mccarthy, Antonio Desmond. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion en Osteospatias y Metabolismo Mineral; Argentina
description Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with Diabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the in vitro alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5 M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8 M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5142
Gangoiti, Maria Virginia; Anbinder, Pablo Sebastián; Cortizo, Ana Maria; Mccarthy, Antonio Desmond; Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate; Elsevier; Acta Histochemica; 115; 7; 9-2013; 649-657
0065-1281
url http://hdl.handle.net/11336/5142
identifier_str_mv Gangoiti, Maria Virginia; Anbinder, Pablo Sebastián; Cortizo, Ana Maria; Mccarthy, Antonio Desmond; Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate; Elsevier; Acta Histochemica; 115; 7; 9-2013; 649-657
0065-1281
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0065128113000184
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.acthis.2013.01.004
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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