Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate

Autores
Gangoitia, María Virginia; Anbinderb, Pablo Sebastián; Cortizo, Ana María; McCarthy, Antonio Desmond
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients withDiabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated thein vitroalterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
Materia
Ciencias Médicas y de la Salud
Osteoblast
Advanced glycation endproducts
Bisphosphonate
Apoptosis
Actin cytoskeleton
Environmental scanning electron microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4759

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network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronateGangoitia, María VirginiaAnbinderb, Pablo SebastiánCortizo, Ana MaríaMcCarthy, Antonio DesmondCiencias Médicas y de la SaludOsteoblastAdvanced glycation endproductsBisphosphonateApoptosisActin cytoskeletonEnvironmental scanning electron microscopyAdvanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with<em>Diabetes mellitus</em>, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the<em>in vitro</em>alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4759enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:50Zoai:digital.cic.gba.gob.ar:11746/4759Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:50.651CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
spellingShingle Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
Gangoitia, María Virginia
Ciencias Médicas y de la Salud
Osteoblast
Advanced glycation endproducts
Bisphosphonate
Apoptosis
Actin cytoskeleton
Environmental scanning electron microscopy
title_short Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_full Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_fullStr Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_full_unstemmed Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
title_sort Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
dc.creator.none.fl_str_mv Gangoitia, María Virginia
Anbinderb, Pablo Sebastián
Cortizo, Ana María
McCarthy, Antonio Desmond
author Gangoitia, María Virginia
author_facet Gangoitia, María Virginia
Anbinderb, Pablo Sebastián
Cortizo, Ana María
McCarthy, Antonio Desmond
author_role author
author2 Anbinderb, Pablo Sebastián
Cortizo, Ana María
McCarthy, Antonio Desmond
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas y de la Salud
Osteoblast
Advanced glycation endproducts
Bisphosphonate
Apoptosis
Actin cytoskeleton
Environmental scanning electron microscopy
topic Ciencias Médicas y de la Salud
Osteoblast
Advanced glycation endproducts
Bisphosphonate
Apoptosis
Actin cytoskeleton
Environmental scanning electron microscopy
dc.description.none.fl_txt_mv Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with<em>Diabetes mellitus</em>, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the<em>in vitro</em>alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
description Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with<em>Diabetes mellitus</em>, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the<em>in vitro</em>alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4759
url https://digital.cic.gba.gob.ar/handle/11746/4759
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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