Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate
- Autores
- Gangoitia, María Virginia; Anbinderb, Pablo Sebastián; Cortizo, Ana María; McCarthy, Antonio Desmond
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients withDiabetes mellitus, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated thein vitroalterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.
- Materia
-
Ciencias Médicas y de la Salud
Osteoblast
Advanced glycation endproducts
Bisphosphonate
Apoptosis
Actin cytoskeleton
Environmental scanning electron microscopy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/4759
Ver los metadatos del registro completo
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Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronateGangoitia, María VirginiaAnbinderb, Pablo SebastiánCortizo, Ana MaríaMcCarthy, Antonio DesmondCiencias Médicas y de la SaludOsteoblastAdvanced glycation endproductsBisphosphonateApoptosisActin cytoskeletonEnvironmental scanning electron microscopyAdvanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with<em>Diabetes mellitus</em>, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the<em>in vitro</em>alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts.2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4759enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:50Zoai:digital.cic.gba.gob.ar:11746/4759Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:50.651CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
title |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
spellingShingle |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate Gangoitia, María Virginia Ciencias Médicas y de la Salud Osteoblast Advanced glycation endproducts Bisphosphonate Apoptosis Actin cytoskeleton Environmental scanning electron microscopy |
title_short |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
title_full |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
title_fullStr |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
title_full_unstemmed |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
title_sort |
Morphological changes induced by advanced glycation endproducts in osteoblastic cells: Effects of co-incubation with alendronate |
dc.creator.none.fl_str_mv |
Gangoitia, María Virginia Anbinderb, Pablo Sebastián Cortizo, Ana María McCarthy, Antonio Desmond |
author |
Gangoitia, María Virginia |
author_facet |
Gangoitia, María Virginia Anbinderb, Pablo Sebastián Cortizo, Ana María McCarthy, Antonio Desmond |
author_role |
author |
author2 |
Anbinderb, Pablo Sebastián Cortizo, Ana María McCarthy, Antonio Desmond |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Médicas y de la Salud Osteoblast Advanced glycation endproducts Bisphosphonate Apoptosis Actin cytoskeleton Environmental scanning electron microscopy |
topic |
Ciencias Médicas y de la Salud Osteoblast Advanced glycation endproducts Bisphosphonate Apoptosis Actin cytoskeleton Environmental scanning electron microscopy |
dc.description.none.fl_txt_mv |
Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with<em>Diabetes mellitus</em>, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the<em>in vitro</em>alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts. |
description |
Advanced glycation endproducts (AGEs) accumulate with age in various tissues, and are further increased in patients with<em>Diabetes mellitus</em>, in which they are believed to contribute to the development and progression of chronic complications that include a decrease in bone quality. Bisphosphonates are anti-osteoporotic drugs that have been used for the treatment of patients with diabetic bone alterations, although with contradictory results. In the present study, we have evaluated the<em>in vitro</em>alterations on osteoblastic morphology by environmental scanning electron microscopy, in actin cytoskeleton and apoptosis induced by AGEs, as well as the modulation of these effects by alendronate (an N-containing bisphosphonate). Our present results provide evidence for disruption induced by AGEs of the osteoblastic actin cytoskeleton (geodesic domes) and significant alterations in cell morphology with a decrease in cell-substratum interactions leading to an increase in apoptosis of osteoblasts and a decrease in osteoblastic proliferation. High concentrations of alendronate (10−5M, such as could be expected in an osteoclastic lacuna) further increase osteoblastic morphological and cytoskeletal alterations. However, low doses of alendronate (10−8M, compatible with extracellular fluid levels to which an osteoblast could be exposed for most of its life cycle) do not affect cell morphology, and in addition are able to prevent AGEs-induced alterations and consequently apoptosis of osteoblasts. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/4759 |
url |
https://digital.cic.gba.gob.ar/handle/11746/4759 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
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CIC Digital (CICBA) |
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CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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