Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile

Autores
Campanini Salinas, Javier; Opitz Ríos, Catherine; Sagredo Mella, John A.; Contreras Sanchez, Danilo; Giménez, Matías; Páez, Paula Andrea; Tarifa, Maria Clara; Rubio, Nataly D.; Medina, Daniel A.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antimicrobial resistance has been stated to be a global health problem. In Chile, the use of antibiotics should be declared by medical prescription, but it is unknown what happens to the drugs once the treatment ends. Among the possibilities for their disposal are the trash or the drain; regardless of which scenario arises, antibiotics could accumulate in the environment, stimulating the emergence of antimicrobial resistance mechanisms and their transfer between microorganisms. Unfortunately, sometimes wastewater ends up in bodies of water, due to the dragging of elements by rain, or by the presence of illegal water discharges. In this work, shotgun metagenomics was used to elucidate the functional and microbial composition of biohazard elements in the bay of Puerto Varas City, Chile. As expected, a high diversity of microorganisms was found, including bacterial elements described as human or animal pathogens. Also, a diverse repertory of antimicrobial resistant genes (ARGs) was detected, which confers mainly resistance to macrolides, beta-lactams, and tetracyclines, consistent with the families of antibiotics most used in Chile. Similar ARGs were identified in DNA mobile elements. In addition, we tested the antimicrobial susceptibility in 14 bacterial strains isolated from Llanquihue Lake. This is the first report of the presence of genomic elements that could constitute a health problem, considering the importance of the interconnection between environmental, animal, and human health, a concept known as One Health.
Fil: Campanini Salinas, Javier. Universidad San Sebastian.; Chile
Fil: Opitz Ríos, Catherine. Universidad San Sebastian.; Chile
Fil: Sagredo Mella, John A.. Universidad San Sebastian.; Chile
Fil: Contreras Sanchez, Danilo. Universidad San Sebastian.; Chile
Fil: Giménez, Matías. Institut Pasteur Montevideo; Uruguay
Fil: Páez, Paula Andrea. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Tarifa, Maria Clara. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Rubio, Nataly D.. Universidad San Sebastian.; Chile
Fil: Medina, Daniel A.. Universidad San Sebastian.; Chile
Materia
ANTIMICROBIAL RESISTANCE
METAGENOMICS
DNA MOBILE ELEMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241635
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/241635
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, ChileCampanini Salinas, JavierOpitz Ríos, CatherineSagredo Mella, John A.Contreras Sanchez, DaniloGiménez, MatíasPáez, Paula AndreaTarifa, Maria ClaraRubio, Nataly D.Medina, Daniel A.ANTIMICROBIAL RESISTANCEMETAGENOMICSDNA MOBILE ELEMENTShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Antimicrobial resistance has been stated to be a global health problem. In Chile, the use of antibiotics should be declared by medical prescription, but it is unknown what happens to the drugs once the treatment ends. Among the possibilities for their disposal are the trash or the drain; regardless of which scenario arises, antibiotics could accumulate in the environment, stimulating the emergence of antimicrobial resistance mechanisms and their transfer between microorganisms. Unfortunately, sometimes wastewater ends up in bodies of water, due to the dragging of elements by rain, or by the presence of illegal water discharges. In this work, shotgun metagenomics was used to elucidate the functional and microbial composition of biohazard elements in the bay of Puerto Varas City, Chile. As expected, a high diversity of microorganisms was found, including bacterial elements described as human or animal pathogens. Also, a diverse repertory of antimicrobial resistant genes (ARGs) was detected, which confers mainly resistance to macrolides, beta-lactams, and tetracyclines, consistent with the families of antibiotics most used in Chile. Similar ARGs were identified in DNA mobile elements. In addition, we tested the antimicrobial susceptibility in 14 bacterial strains isolated from Llanquihue Lake. This is the first report of the presence of genomic elements that could constitute a health problem, considering the importance of the interconnection between environmental, animal, and human health, a concept known as One Health.Fil: Campanini Salinas, Javier. Universidad San Sebastian.; ChileFil: Opitz Ríos, Catherine. Universidad San Sebastian.; ChileFil: Sagredo Mella, John A.. Universidad San Sebastian.; ChileFil: Contreras Sanchez, Danilo. Universidad San Sebastian.; ChileFil: Giménez, Matías. Institut Pasteur Montevideo; UruguayFil: Páez, Paula Andrea. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Tarifa, Maria Clara. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Rubio, Nataly D.. Universidad San Sebastian.; ChileFil: Medina, Daniel A.. Universidad San Sebastian.; ChileMDPI2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241635Campanini Salinas, Javier; Opitz Ríos, Catherine; Sagredo Mella, John A.; Contreras Sanchez, Danilo; Giménez, Matías; et al.; Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile; MDPI; Antibiotics; 13; 7; 7-2024; 1-142079-6382CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/13/7/679info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics13070679info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T11:46:00Zoai:ri.conicet.gov.ar:11336/241635instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 11:46:00.734CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
title Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
spellingShingle Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
Campanini Salinas, Javier
ANTIMICROBIAL RESISTANCE
METAGENOMICS
DNA MOBILE ELEMENTS
title_short Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
title_full Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
title_fullStr Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
title_full_unstemmed Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
title_sort Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile
dc.creator.none.fl_str_mv Campanini Salinas, Javier
Opitz Ríos, Catherine
Sagredo Mella, John A.
Contreras Sanchez, Danilo
Giménez, Matías
Páez, Paula Andrea
Tarifa, Maria Clara
Rubio, Nataly D.
Medina, Daniel A.
author Campanini Salinas, Javier
author_facet Campanini Salinas, Javier
Opitz Ríos, Catherine
Sagredo Mella, John A.
Contreras Sanchez, Danilo
Giménez, Matías
Páez, Paula Andrea
Tarifa, Maria Clara
Rubio, Nataly D.
Medina, Daniel A.
author_role author
author2 Opitz Ríos, Catherine
Sagredo Mella, John A.
Contreras Sanchez, Danilo
Giménez, Matías
Páez, Paula Andrea
Tarifa, Maria Clara
Rubio, Nataly D.
Medina, Daniel A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL RESISTANCE
METAGENOMICS
DNA MOBILE ELEMENTS
topic ANTIMICROBIAL RESISTANCE
METAGENOMICS
DNA MOBILE ELEMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Antimicrobial resistance has been stated to be a global health problem. In Chile, the use of antibiotics should be declared by medical prescription, but it is unknown what happens to the drugs once the treatment ends. Among the possibilities for their disposal are the trash or the drain; regardless of which scenario arises, antibiotics could accumulate in the environment, stimulating the emergence of antimicrobial resistance mechanisms and their transfer between microorganisms. Unfortunately, sometimes wastewater ends up in bodies of water, due to the dragging of elements by rain, or by the presence of illegal water discharges. In this work, shotgun metagenomics was used to elucidate the functional and microbial composition of biohazard elements in the bay of Puerto Varas City, Chile. As expected, a high diversity of microorganisms was found, including bacterial elements described as human or animal pathogens. Also, a diverse repertory of antimicrobial resistant genes (ARGs) was detected, which confers mainly resistance to macrolides, beta-lactams, and tetracyclines, consistent with the families of antibiotics most used in Chile. Similar ARGs were identified in DNA mobile elements. In addition, we tested the antimicrobial susceptibility in 14 bacterial strains isolated from Llanquihue Lake. This is the first report of the presence of genomic elements that could constitute a health problem, considering the importance of the interconnection between environmental, animal, and human health, a concept known as One Health.
Fil: Campanini Salinas, Javier. Universidad San Sebastian.; Chile
Fil: Opitz Ríos, Catherine. Universidad San Sebastian.; Chile
Fil: Sagredo Mella, John A.. Universidad San Sebastian.; Chile
Fil: Contreras Sanchez, Danilo. Universidad San Sebastian.; Chile
Fil: Giménez, Matías. Institut Pasteur Montevideo; Uruguay
Fil: Páez, Paula Andrea. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Tarifa, Maria Clara. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Rubio, Nataly D.. Universidad San Sebastian.; Chile
Fil: Medina, Daniel A.. Universidad San Sebastian.; Chile
description Antimicrobial resistance has been stated to be a global health problem. In Chile, the use of antibiotics should be declared by medical prescription, but it is unknown what happens to the drugs once the treatment ends. Among the possibilities for their disposal are the trash or the drain; regardless of which scenario arises, antibiotics could accumulate in the environment, stimulating the emergence of antimicrobial resistance mechanisms and their transfer between microorganisms. Unfortunately, sometimes wastewater ends up in bodies of water, due to the dragging of elements by rain, or by the presence of illegal water discharges. In this work, shotgun metagenomics was used to elucidate the functional and microbial composition of biohazard elements in the bay of Puerto Varas City, Chile. As expected, a high diversity of microorganisms was found, including bacterial elements described as human or animal pathogens. Also, a diverse repertory of antimicrobial resistant genes (ARGs) was detected, which confers mainly resistance to macrolides, beta-lactams, and tetracyclines, consistent with the families of antibiotics most used in Chile. Similar ARGs were identified in DNA mobile elements. In addition, we tested the antimicrobial susceptibility in 14 bacterial strains isolated from Llanquihue Lake. This is the first report of the presence of genomic elements that could constitute a health problem, considering the importance of the interconnection between environmental, animal, and human health, a concept known as One Health.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241635
Campanini Salinas, Javier; Opitz Ríos, Catherine; Sagredo Mella, John A.; Contreras Sanchez, Danilo; Giménez, Matías; et al.; Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile; MDPI; Antibiotics; 13; 7; 7-2024; 1-14
2079-6382
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241635
identifier_str_mv Campanini Salinas, Javier; Opitz Ríos, Catherine; Sagredo Mella, John A.; Contreras Sanchez, Danilo; Giménez, Matías; et al.; Antimicrobial Resistance Elements in Coastal Water of Llanquihue Lake, Chile; MDPI; Antibiotics; 13; 7; 7-2024; 1-14
2079-6382
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/13/7/679
info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics13070679
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1854320875917017088
score 13.113929

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