Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms

Autores
Gambino, Anahí Samanta; Déraspe, Maxime; Alvarez, Verónica Elizabeth; Quiroga, María Paula; Corbeil, Jacques; Roy, Paul H.; Centron, Daniela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS1. Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae. Maintenance studies showed that the three class 1 integrons were maintained over 1 month without antimicrobial pressure. Since S. marcescens is considered a relevant nosocomial pathogen that can have a wide range of niches - human, plant, animal, soil and inanimate surfaces, our findings support the ability of this species to capture, maintain and spread a broad variety of antimicrobial resistance elements.
Fil: Gambino, Anahí Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Déraspe, Maxime. Laval University; Canadá
Fil: Alvarez, Verónica Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Quiroga, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Corbeil, Jacques. Laval University; Canadá
Fil: Roy, Paul H.. Laval University; Canadá
Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Materia
SERRATIA MARCESCENS
RESERVOIR AND SOURCE
ANTIBIOTIC RESISTANCE MECHANISMS
GENETIC ELEMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168195
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/168195
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanismsGambino, Anahí SamantaDéraspe, MaximeAlvarez, Verónica ElizabethQuiroga, María PaulaCorbeil, JacquesRoy, Paul H.Centron, DanielaSERRATIA MARCESCENSRESERVOIR AND SOURCEANTIBIOTIC RESISTANCE MECHANISMSGENETIC ELEMENTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS1. Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae. Maintenance studies showed that the three class 1 integrons were maintained over 1 month without antimicrobial pressure. Since S. marcescens is considered a relevant nosocomial pathogen that can have a wide range of niches - human, plant, animal, soil and inanimate surfaces, our findings support the ability of this species to capture, maintain and spread a broad variety of antimicrobial resistance elements.Fil: Gambino, Anahí Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Déraspe, Maxime. Laval University; CanadáFil: Alvarez, Verónica Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Quiroga, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Corbeil, Jacques. Laval University; CanadáFil: Roy, Paul H.. Laval University; CanadáFil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaWiley Blackwell Publishing, Inc2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168195Gambino, Anahí Samanta; Déraspe, Maxime; Alvarez, Verónica Elizabeth; Quiroga, María Paula; Corbeil, Jacques; et al.; Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 368; 14; 7-2021; 1-101574-6968CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/doi/10.1093/femsle/fnab086/6321840info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnab086info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T11:53:47Zoai:ri.conicet.gov.ar:11336/168195instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 11:53:47.988CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
title Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
spellingShingle Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
Gambino, Anahí Samanta
SERRATIA MARCESCENS
RESERVOIR AND SOURCE
ANTIBIOTIC RESISTANCE MECHANISMS
GENETIC ELEMENTS
title_short Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
title_full Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
title_fullStr Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
title_full_unstemmed Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
title_sort Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms
dc.creator.none.fl_str_mv Gambino, Anahí Samanta
Déraspe, Maxime
Alvarez, Verónica Elizabeth
Quiroga, María Paula
Corbeil, Jacques
Roy, Paul H.
Centron, Daniela
author Gambino, Anahí Samanta
author_facet Gambino, Anahí Samanta
Déraspe, Maxime
Alvarez, Verónica Elizabeth
Quiroga, María Paula
Corbeil, Jacques
Roy, Paul H.
Centron, Daniela
author_role author
author2 Déraspe, Maxime
Alvarez, Verónica Elizabeth
Quiroga, María Paula
Corbeil, Jacques
Roy, Paul H.
Centron, Daniela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SERRATIA MARCESCENS
RESERVOIR AND SOURCE
ANTIBIOTIC RESISTANCE MECHANISMS
GENETIC ELEMENTS
topic SERRATIA MARCESCENS
RESERVOIR AND SOURCE
ANTIBIOTIC RESISTANCE MECHANISMS
GENETIC ELEMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS1. Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae. Maintenance studies showed that the three class 1 integrons were maintained over 1 month without antimicrobial pressure. Since S. marcescens is considered a relevant nosocomial pathogen that can have a wide range of niches - human, plant, animal, soil and inanimate surfaces, our findings support the ability of this species to capture, maintain and spread a broad variety of antimicrobial resistance elements.
Fil: Gambino, Anahí Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Déraspe, Maxime. Laval University; Canadá
Fil: Alvarez, Verónica Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Quiroga, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Corbeil, Jacques. Laval University; Canadá
Fil: Roy, Paul H.. Laval University; Canadá
Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
description Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS1. Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae. Maintenance studies showed that the three class 1 integrons were maintained over 1 month without antimicrobial pressure. Since S. marcescens is considered a relevant nosocomial pathogen that can have a wide range of niches - human, plant, animal, soil and inanimate surfaces, our findings support the ability of this species to capture, maintain and spread a broad variety of antimicrobial resistance elements.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168195
Gambino, Anahí Samanta; Déraspe, Maxime; Alvarez, Verónica Elizabeth; Quiroga, María Paula; Corbeil, Jacques; et al.; Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 368; 14; 7-2021; 1-10
1574-6968
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168195
identifier_str_mv Gambino, Anahí Samanta; Déraspe, Maxime; Alvarez, Verónica Elizabeth; Quiroga, María Paula; Corbeil, Jacques; et al.; Serratia marcescens sch909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 368; 14; 7-2021; 1-10
1574-6968
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/doi/10.1093/femsle/fnab086/6321840
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnab086
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.113929

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