ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host

Autores
Parmeciano Di Noto, Gisela Paula; Iriarte, Andrés; Ramírez, María Soledad; Centron, Daniela; Quiroga, Cecilia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Integrative and conjugative elements (ICEs) are mobile genetic elements that contribute to horizontal gene transfer. The aim of this work was to study different types of ICEs in clinical isolates of the emergent pathogen Shewanella spp., to compare their transfer efficiency and their ability to integrate a new host. Here we show that 3 out of 10 clinical isolates contained an ICE. Two of these elements were similar to ICEs from the SXT/R391 family and the other one was similar to ICESh95, a hybrid platform. Mating assays showed that these elements co-exist for several generations in the same host. Furthermore, transfer rates and competition assays between ICESh95 and ICESh392, an SXT-like element, suggest that the latter has evolved into a well-oiled machine that efficiently spread to different bacteria. Our results provide strong evidence of the role that ICEs play in the dissemination of genetic traits in nature and the implications that they have in the global threat of antimicrobial resistance.
Fil: Parmeciano Di Noto, Gisela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Iriarte, Andrés. Universidad de la República; Uruguay
Fil: Ramírez, María Soledad. California State University; Estados Unidos
Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Quiroga, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Materia
INTEGRATIVE CONJUGATIVE ELEMENTS
ANTIMICROBIAL RESISTANCE
HORIZONTAL TRANSFER EVENTS
COMPETITION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132650
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New HostParmeciano Di Noto, Gisela PaulaIriarte, AndrésRamírez, María SoledadCentron, DanielaQuiroga, CeciliaINTEGRATIVE CONJUGATIVE ELEMENTSANTIMICROBIAL RESISTANCEHORIZONTAL TRANSFER EVENTSCOMPETITIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Integrative and conjugative elements (ICEs) are mobile genetic elements that contribute to horizontal gene transfer. The aim of this work was to study different types of ICEs in clinical isolates of the emergent pathogen Shewanella spp., to compare their transfer efficiency and their ability to integrate a new host. Here we show that 3 out of 10 clinical isolates contained an ICE. Two of these elements were similar to ICEs from the SXT/R391 family and the other one was similar to ICESh95, a hybrid platform. Mating assays showed that these elements co-exist for several generations in the same host. Furthermore, transfer rates and competition assays between ICESh95 and ICESh392, an SXT-like element, suggest that the latter has evolved into a well-oiled machine that efficiently spread to different bacteria. Our results provide strong evidence of the role that ICEs play in the dissemination of genetic traits in nature and the implications that they have in the global threat of antimicrobial resistance.Fil: Parmeciano Di Noto, Gisela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Iriarte, Andrés. Universidad de la República; UruguayFil: Ramírez, María Soledad. California State University; Estados UnidosFil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Quiroga, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaNature Publishing Group2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132650Parmeciano Di Noto, Gisela Paula; Iriarte, Andrés; Ramírez, María Soledad; Centron, Daniela; Quiroga, Cecilia; ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host; Nature Publishing Group; Scientific Reports; 9; 1; 12-2019; 1-72045-23222045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-019-44312-1info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-44312-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:40:30Zoai:ri.conicet.gov.ar:11336/132650instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:40:31.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
title ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
spellingShingle ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
Parmeciano Di Noto, Gisela Paula
INTEGRATIVE CONJUGATIVE ELEMENTS
ANTIMICROBIAL RESISTANCE
HORIZONTAL TRANSFER EVENTS
COMPETITION
title_short ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
title_full ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
title_fullStr ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
title_full_unstemmed ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
title_sort ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host
dc.creator.none.fl_str_mv Parmeciano Di Noto, Gisela Paula
Iriarte, Andrés
Ramírez, María Soledad
Centron, Daniela
Quiroga, Cecilia
author Parmeciano Di Noto, Gisela Paula
author_facet Parmeciano Di Noto, Gisela Paula
Iriarte, Andrés
Ramírez, María Soledad
Centron, Daniela
Quiroga, Cecilia
author_role author
author2 Iriarte, Andrés
Ramírez, María Soledad
Centron, Daniela
Quiroga, Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv INTEGRATIVE CONJUGATIVE ELEMENTS
ANTIMICROBIAL RESISTANCE
HORIZONTAL TRANSFER EVENTS
COMPETITION
topic INTEGRATIVE CONJUGATIVE ELEMENTS
ANTIMICROBIAL RESISTANCE
HORIZONTAL TRANSFER EVENTS
COMPETITION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Integrative and conjugative elements (ICEs) are mobile genetic elements that contribute to horizontal gene transfer. The aim of this work was to study different types of ICEs in clinical isolates of the emergent pathogen Shewanella spp., to compare their transfer efficiency and their ability to integrate a new host. Here we show that 3 out of 10 clinical isolates contained an ICE. Two of these elements were similar to ICEs from the SXT/R391 family and the other one was similar to ICESh95, a hybrid platform. Mating assays showed that these elements co-exist for several generations in the same host. Furthermore, transfer rates and competition assays between ICESh95 and ICESh392, an SXT-like element, suggest that the latter has evolved into a well-oiled machine that efficiently spread to different bacteria. Our results provide strong evidence of the role that ICEs play in the dissemination of genetic traits in nature and the implications that they have in the global threat of antimicrobial resistance.
Fil: Parmeciano Di Noto, Gisela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Iriarte, Andrés. Universidad de la República; Uruguay
Fil: Ramírez, María Soledad. California State University; Estados Unidos
Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Quiroga, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
description Integrative and conjugative elements (ICEs) are mobile genetic elements that contribute to horizontal gene transfer. The aim of this work was to study different types of ICEs in clinical isolates of the emergent pathogen Shewanella spp., to compare their transfer efficiency and their ability to integrate a new host. Here we show that 3 out of 10 clinical isolates contained an ICE. Two of these elements were similar to ICEs from the SXT/R391 family and the other one was similar to ICESh95, a hybrid platform. Mating assays showed that these elements co-exist for several generations in the same host. Furthermore, transfer rates and competition assays between ICESh95 and ICESh392, an SXT-like element, suggest that the latter has evolved into a well-oiled machine that efficiently spread to different bacteria. Our results provide strong evidence of the role that ICEs play in the dissemination of genetic traits in nature and the implications that they have in the global threat of antimicrobial resistance.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132650
Parmeciano Di Noto, Gisela Paula; Iriarte, Andrés; Ramírez, María Soledad; Centron, Daniela; Quiroga, Cecilia; ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host; Nature Publishing Group; Scientific Reports; 9; 1; 12-2019; 1-7
2045-2322
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132650
identifier_str_mv Parmeciano Di Noto, Gisela Paula; Iriarte, Andrés; Ramírez, María Soledad; Centron, Daniela; Quiroga, Cecilia; ICE SXT vs. ICESh95: Co-existence of Integrative and Conjugative Elements and Competition for a New Host; Nature Publishing Group; Scientific Reports; 9; 1; 12-2019; 1-7
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-019-44312-1
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-44312-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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