Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines

Autores
Zucchini, Cinzia; Rocchi, Anna; Manara, María Cristina; de Sanctis, Paola; Capanni, Cristina; Bianchini, Michele; Carinci, Paolo; Scotlandi, Katia; Valvassori, Luisa
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metastasis is the most frequent cause of death among patients with osteosarcoma. We have previously demonstrated in independent experiments that the forced expression of L/B/K ALP and CD99 in U-2 OS osteosarcoma cell lines markedly reduces the metastatic ability of these cancer cells. This behavior makes these cell lines a useful model to assess the intersection of multiple and independent gene expression signatures concerning the biological problem of dissemination. With the aim to characterize a common transcriptional profile reflecting the essential features of metastatic behavior, we employed cDNA microarrays to compare the gene expression profiles of L/B/K ALP- and CD99-transfected osteosarcoma clones showing low metastatic ability with those of osteosarcoma cell lines showing contrasting behavior. Changes in gene expression were validated by real-time PCR and immunohistochemistry in independent samples. In our study we identified several differentially expressed genes (GADD45á, VCP, DHX9, survivin, á-catulin, ARPC1B) related to growth arrest and apoptosis. Most of these genes are functionally related with the nuclear factor (NF)-êB cell survival pathway that appeared to be inhibited in the less malignant osteosarcoma cells. Hence, we propose the inhibition of the NF-êB pathway as a rational strategy for effective management of human osteosarcoma.
Fil: Zucchini, Cinzia. Università di Bologna; Italia
Fil: Rocchi, Anna. Istituti Ortopedici Rizzoli; Italia
Fil: Manara, María Cristina. Istituti Ortopedici Rizzoli; Italia
Fil: de Sanctis, Paola. Università di Bologna; Italia
Fil: Capanni, Cristina. Università di Bologna; Italia
Fil: Bianchini, Michele. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Università di Bologna; Italia
Fil: Carinci, Paolo. Università di Bologna; Italia
Fil: Scotlandi, Katia. Istituti Ortopedici Rizzoli; Italia
Fil: Valvassori, Luisa. Università di Bologna; Italia
Materia
MICROARRAYS
OSTEOSARCOMA
METASTASIS
L/B/K/ALP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244529
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244529
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell linesZucchini, CinziaRocchi, AnnaManara, María Cristinade Sanctis, PaolaCapanni, CristinaBianchini, MicheleCarinci, PaoloScotlandi, KatiaValvassori, LuisaMICROARRAYSOSTEOSARCOMAMETASTASISL/B/K/ALPhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Metastasis is the most frequent cause of death among patients with osteosarcoma. We have previously demonstrated in independent experiments that the forced expression of L/B/K ALP and CD99 in U-2 OS osteosarcoma cell lines markedly reduces the metastatic ability of these cancer cells. This behavior makes these cell lines a useful model to assess the intersection of multiple and independent gene expression signatures concerning the biological problem of dissemination. With the aim to characterize a common transcriptional profile reflecting the essential features of metastatic behavior, we employed cDNA microarrays to compare the gene expression profiles of L/B/K ALP- and CD99-transfected osteosarcoma clones showing low metastatic ability with those of osteosarcoma cell lines showing contrasting behavior. Changes in gene expression were validated by real-time PCR and immunohistochemistry in independent samples. In our study we identified several differentially expressed genes (GADD45á, VCP, DHX9, survivin, á-catulin, ARPC1B) related to growth arrest and apoptosis. Most of these genes are functionally related with the nuclear factor (NF)-êB cell survival pathway that appeared to be inhibited in the less malignant osteosarcoma cells. Hence, we propose the inhibition of the NF-êB pathway as a rational strategy for effective management of human osteosarcoma.Fil: Zucchini, Cinzia. Università di Bologna; ItaliaFil: Rocchi, Anna. Istituti Ortopedici Rizzoli; ItaliaFil: Manara, María Cristina. Istituti Ortopedici Rizzoli; ItaliaFil: de Sanctis, Paola. Università di Bologna; ItaliaFil: Capanni, Cristina. Università di Bologna; ItaliaFil: Bianchini, Michele. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Università di Bologna; ItaliaFil: Carinci, Paolo. Università di Bologna; ItaliaFil: Scotlandi, Katia. Istituti Ortopedici Rizzoli; ItaliaFil: Valvassori, Luisa. Università di Bologna; ItaliaSpandidos Publications2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244529Zucchini, Cinzia; Rocchi, Anna; Manara, María Cristina; de Sanctis, Paola; Capanni, Cristina; et al.; Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines; Spandidos Publications; International Journal of Oncology; 32; 1; 1-2008; 17-311019-6439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/ijo/32/1/17info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:27Zoai:ri.conicet.gov.ar:11336/244529instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:27.888CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
title Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
spellingShingle Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
Zucchini, Cinzia
MICROARRAYS
OSTEOSARCOMA
METASTASIS
L/B/K/ALP
title_short Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
title_full Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
title_fullStr Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
title_full_unstemmed Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
title_sort Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines
dc.creator.none.fl_str_mv Zucchini, Cinzia
Rocchi, Anna
Manara, María Cristina
de Sanctis, Paola
Capanni, Cristina
Bianchini, Michele
Carinci, Paolo
Scotlandi, Katia
Valvassori, Luisa
author Zucchini, Cinzia
author_facet Zucchini, Cinzia
Rocchi, Anna
Manara, María Cristina
de Sanctis, Paola
Capanni, Cristina
Bianchini, Michele
Carinci, Paolo
Scotlandi, Katia
Valvassori, Luisa
author_role author
author2 Rocchi, Anna
Manara, María Cristina
de Sanctis, Paola
Capanni, Cristina
Bianchini, Michele
Carinci, Paolo
Scotlandi, Katia
Valvassori, Luisa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROARRAYS
OSTEOSARCOMA
METASTASIS
L/B/K/ALP
topic MICROARRAYS
OSTEOSARCOMA
METASTASIS
L/B/K/ALP
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Metastasis is the most frequent cause of death among patients with osteosarcoma. We have previously demonstrated in independent experiments that the forced expression of L/B/K ALP and CD99 in U-2 OS osteosarcoma cell lines markedly reduces the metastatic ability of these cancer cells. This behavior makes these cell lines a useful model to assess the intersection of multiple and independent gene expression signatures concerning the biological problem of dissemination. With the aim to characterize a common transcriptional profile reflecting the essential features of metastatic behavior, we employed cDNA microarrays to compare the gene expression profiles of L/B/K ALP- and CD99-transfected osteosarcoma clones showing low metastatic ability with those of osteosarcoma cell lines showing contrasting behavior. Changes in gene expression were validated by real-time PCR and immunohistochemistry in independent samples. In our study we identified several differentially expressed genes (GADD45á, VCP, DHX9, survivin, á-catulin, ARPC1B) related to growth arrest and apoptosis. Most of these genes are functionally related with the nuclear factor (NF)-êB cell survival pathway that appeared to be inhibited in the less malignant osteosarcoma cells. Hence, we propose the inhibition of the NF-êB pathway as a rational strategy for effective management of human osteosarcoma.
Fil: Zucchini, Cinzia. Università di Bologna; Italia
Fil: Rocchi, Anna. Istituti Ortopedici Rizzoli; Italia
Fil: Manara, María Cristina. Istituti Ortopedici Rizzoli; Italia
Fil: de Sanctis, Paola. Università di Bologna; Italia
Fil: Capanni, Cristina. Università di Bologna; Italia
Fil: Bianchini, Michele. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Università di Bologna; Italia
Fil: Carinci, Paolo. Università di Bologna; Italia
Fil: Scotlandi, Katia. Istituti Ortopedici Rizzoli; Italia
Fil: Valvassori, Luisa. Università di Bologna; Italia
description Metastasis is the most frequent cause of death among patients with osteosarcoma. We have previously demonstrated in independent experiments that the forced expression of L/B/K ALP and CD99 in U-2 OS osteosarcoma cell lines markedly reduces the metastatic ability of these cancer cells. This behavior makes these cell lines a useful model to assess the intersection of multiple and independent gene expression signatures concerning the biological problem of dissemination. With the aim to characterize a common transcriptional profile reflecting the essential features of metastatic behavior, we employed cDNA microarrays to compare the gene expression profiles of L/B/K ALP- and CD99-transfected osteosarcoma clones showing low metastatic ability with those of osteosarcoma cell lines showing contrasting behavior. Changes in gene expression were validated by real-time PCR and immunohistochemistry in independent samples. In our study we identified several differentially expressed genes (GADD45á, VCP, DHX9, survivin, á-catulin, ARPC1B) related to growth arrest and apoptosis. Most of these genes are functionally related with the nuclear factor (NF)-êB cell survival pathway that appeared to be inhibited in the less malignant osteosarcoma cells. Hence, we propose the inhibition of the NF-êB pathway as a rational strategy for effective management of human osteosarcoma.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244529
Zucchini, Cinzia; Rocchi, Anna; Manara, María Cristina; de Sanctis, Paola; Capanni, Cristina; et al.; Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines; Spandidos Publications; International Journal of Oncology; 32; 1; 1-2008; 17-31
1019-6439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244529
identifier_str_mv Zucchini, Cinzia; Rocchi, Anna; Manara, María Cristina; de Sanctis, Paola; Capanni, Cristina; et al.; Apoptotic genes as potential markers of metastatic phenotype in human osteosarcoma cell lines; Spandidos Publications; International Journal of Oncology; 32; 1; 1-2008; 17-31
1019-6439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/ijo/32/1/17
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spandidos Publications
publisher.none.fl_str_mv Spandidos Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980333577830400
score 12.993085

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