Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
- Autores
- Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; Vargas, Jorge; Torrico, Faustino; Diosque, Patricio; Valente, Vera; Valente, Sebastiao A.; Gaunt, Michael W.
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed.
Fil: Llewellyn, Martin S.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Miles, Michael A.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Carrasco, Hector Jeronimo. Universidad Central de Venezuela; Venezuela
Fil: Lewis, Michael D.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Yeo, Matthew. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Vargas, Jorge. Centro Nacional de Enfermedades Tropicales; Bolivia
Fil: Torrico, Faustino. Universidad Mayor de San Simón; Bolivia
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Valente, Vera. Instituto Evandro Chagas; Brasil
Fil: Valente, Sebastiao A.. Instituto Evandro Chagas; Brasil
Fil: Gaunt, Michael W.. London School of Hgyene and Tropical Medicine; Reino Unido - Materia
-
Trypanosoma cruzi
Microsatellite
Typing
Phylogeography - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/29958
Ver los metadatos del registro completo
id |
CONICETDig_3cbef9fa766630342448d4789914e8aa |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/29958 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human InfectionLlewellyn, Martin S.Miles, Michael A.Carrasco, Hector JeronimoLewis, Michael D.Yeo, MatthewVargas, JorgeTorrico, FaustinoDiosque, PatricioValente, VeraValente, Sebastiao A.Gaunt, Michael W.Trypanosoma cruziMicrosatelliteTypingPhylogeographyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed.Fil: Llewellyn, Martin S.. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Miles, Michael A.. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Carrasco, Hector Jeronimo. Universidad Central de Venezuela; VenezuelaFil: Lewis, Michael D.. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Yeo, Matthew. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Vargas, Jorge. Centro Nacional de Enfermedades Tropicales; BoliviaFil: Torrico, Faustino. Universidad Mayor de San Simón; BoliviaFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Valente, Vera. Instituto Evandro Chagas; BrasilFil: Valente, Sebastiao A.. Instituto Evandro Chagas; BrasilFil: Gaunt, Michael W.. London School of Hgyene and Tropical Medicine; Reino UnidoPublic Library of Science2009-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29958Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; et al.; Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection; Public Library of Science; Plos Pathogens; 5; 5; 1-5-2009; 1-9; e10004101553-7366CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1000410info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:26Zoai:ri.conicet.gov.ar:11336/29958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:27.168CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
title |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
spellingShingle |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection Llewellyn, Martin S. Trypanosoma cruzi Microsatellite Typing Phylogeography |
title_short |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
title_full |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
title_fullStr |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
title_full_unstemmed |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
title_sort |
Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection |
dc.creator.none.fl_str_mv |
Llewellyn, Martin S. Miles, Michael A. Carrasco, Hector Jeronimo Lewis, Michael D. Yeo, Matthew Vargas, Jorge Torrico, Faustino Diosque, Patricio Valente, Vera Valente, Sebastiao A. Gaunt, Michael W. |
author |
Llewellyn, Martin S. |
author_facet |
Llewellyn, Martin S. Miles, Michael A. Carrasco, Hector Jeronimo Lewis, Michael D. Yeo, Matthew Vargas, Jorge Torrico, Faustino Diosque, Patricio Valente, Vera Valente, Sebastiao A. Gaunt, Michael W. |
author_role |
author |
author2 |
Miles, Michael A. Carrasco, Hector Jeronimo Lewis, Michael D. Yeo, Matthew Vargas, Jorge Torrico, Faustino Diosque, Patricio Valente, Vera Valente, Sebastiao A. Gaunt, Michael W. |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Trypanosoma cruzi Microsatellite Typing Phylogeography |
topic |
Trypanosoma cruzi Microsatellite Typing Phylogeography |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed. Fil: Llewellyn, Martin S.. London School of Hgyene and Tropical Medicine; Reino Unido Fil: Miles, Michael A.. London School of Hgyene and Tropical Medicine; Reino Unido Fil: Carrasco, Hector Jeronimo. Universidad Central de Venezuela; Venezuela Fil: Lewis, Michael D.. London School of Hgyene and Tropical Medicine; Reino Unido Fil: Yeo, Matthew. London School of Hgyene and Tropical Medicine; Reino Unido Fil: Vargas, Jorge. Centro Nacional de Enfermedades Tropicales; Bolivia Fil: Torrico, Faustino. Universidad Mayor de San Simón; Bolivia Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Valente, Vera. Instituto Evandro Chagas; Brasil Fil: Valente, Sebastiao A.. Instituto Evandro Chagas; Brasil Fil: Gaunt, Michael W.. London School of Hgyene and Tropical Medicine; Reino Unido |
description |
Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/29958 Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; et al.; Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection; Public Library of Science; Plos Pathogens; 5; 5; 1-5-2009; 1-9; e1000410 1553-7366 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/29958 |
identifier_str_mv |
Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; et al.; Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection; Public Library of Science; Plos Pathogens; 5; 5; 1-5-2009; 1-9; e1000410 1553-7366 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/reference/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1000410 info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613505143013376 |
score |
13.070432 |