Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection

Autores
Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; Vargas, Jorge; Torrico, Faustino; Diosque, Patricio; Valente, Vera; Valente, Sebastiao A.; Gaunt, Michael W.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed.
Fil: Llewellyn, Martin S.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Miles, Michael A.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Carrasco, Hector Jeronimo. Universidad Central de Venezuela; Venezuela
Fil: Lewis, Michael D.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Yeo, Matthew. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Vargas, Jorge. Centro Nacional de Enfermedades Tropicales; Bolivia
Fil: Torrico, Faustino. Universidad Mayor de San Simón; Bolivia
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Valente, Vera. Instituto Evandro Chagas; Brasil
Fil: Valente, Sebastiao A.. Instituto Evandro Chagas; Brasil
Fil: Gaunt, Michael W.. London School of Hgyene and Tropical Medicine; Reino Unido
Materia
Trypanosoma cruzi
Microsatellite
Typing
Phylogeography
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29958
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29958
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human InfectionLlewellyn, Martin S.Miles, Michael A.Carrasco, Hector JeronimoLewis, Michael D.Yeo, MatthewVargas, JorgeTorrico, FaustinoDiosque, PatricioValente, VeraValente, Sebastiao A.Gaunt, Michael W.Trypanosoma cruziMicrosatelliteTypingPhylogeographyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed.Fil: Llewellyn, Martin S.. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Miles, Michael A.. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Carrasco, Hector Jeronimo. Universidad Central de Venezuela; VenezuelaFil: Lewis, Michael D.. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Yeo, Matthew. London School of Hgyene and Tropical Medicine; Reino UnidoFil: Vargas, Jorge. Centro Nacional de Enfermedades Tropicales; BoliviaFil: Torrico, Faustino. Universidad Mayor de San Simón; BoliviaFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Valente, Vera. Instituto Evandro Chagas; BrasilFil: Valente, Sebastiao A.. Instituto Evandro Chagas; BrasilFil: Gaunt, Michael W.. London School of Hgyene and Tropical Medicine; Reino UnidoPublic Library of Science2009-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29958Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; et al.; Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection; Public Library of Science; Plos Pathogens; 5; 5; 1-5-2009; 1-9; e10004101553-7366CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1000410info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:17:27Zoai:ri.conicet.gov.ar:11336/29958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:17:27.968CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
title Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
spellingShingle Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
Llewellyn, Martin S.
Trypanosoma cruzi
Microsatellite
Typing
Phylogeography
title_short Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
title_full Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
title_fullStr Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
title_full_unstemmed Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
title_sort Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection
dc.creator.none.fl_str_mv Llewellyn, Martin S.
Miles, Michael A.
Carrasco, Hector Jeronimo
Lewis, Michael D.
Yeo, Matthew
Vargas, Jorge
Torrico, Faustino
Diosque, Patricio
Valente, Vera
Valente, Sebastiao A.
Gaunt, Michael W.
author Llewellyn, Martin S.
author_facet Llewellyn, Martin S.
Miles, Michael A.
Carrasco, Hector Jeronimo
Lewis, Michael D.
Yeo, Matthew
Vargas, Jorge
Torrico, Faustino
Diosque, Patricio
Valente, Vera
Valente, Sebastiao A.
Gaunt, Michael W.
author_role author
author2 Miles, Michael A.
Carrasco, Hector Jeronimo
Lewis, Michael D.
Yeo, Matthew
Vargas, Jorge
Torrico, Faustino
Diosque, Patricio
Valente, Vera
Valente, Sebastiao A.
Gaunt, Michael W.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma cruzi
Microsatellite
Typing
Phylogeography
topic Trypanosoma cruzi
Microsatellite
Typing
Phylogeography
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed.
Fil: Llewellyn, Martin S.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Miles, Michael A.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Carrasco, Hector Jeronimo. Universidad Central de Venezuela; Venezuela
Fil: Lewis, Michael D.. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Yeo, Matthew. London School of Hgyene and Tropical Medicine; Reino Unido
Fil: Vargas, Jorge. Centro Nacional de Enfermedades Tropicales; Bolivia
Fil: Torrico, Faustino. Universidad Mayor de San Simón; Bolivia
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Valente, Vera. Instituto Evandro Chagas; Brasil
Fil: Valente, Sebastiao A.. Instituto Evandro Chagas; Brasil
Fil: Gaunt, Michael W.. London School of Hgyene and Tropical Medicine; Reino Unido
description Trypanosoma cruzi is the most important parasitic infection in Latin America and is also genetically highly diverse, with at least six discrete typing units (DTUs) reported: Tc I, IIa, IIb, IIc, IId, and IIe. However, the current six-genotype classification is likely to be a poor reflection of the total genetic diversity present in this undeniably ancient parasite. To determine whether epidemiologically important information is “hidden” at the sub-DTU level, we developed a 48-marker panel of polymorphic microsatellite loci to investigate population structure among 135 samples from across the geographic distribution of TcI. This DTU is the major cause of resurgent human disease in northern South America but also occurs in silvatic triatomine vectors and mammalian reservoir hosts throughout the continent. Based on a total dataset of 12,329 alleles, we demonstrate that silvatic TcI populations are extraordinarily genetically diverse, show spatial structuring on a continental scale, and have undergone recent biogeographic expansion into the southern United States of America. Conversely, the majority of human strains sampled are restricted to two distinct groups characterised by a considerable reduction in genetic diversity with respect to isolates from silvatic sources. In Venezuela, most human isolates showed little identity with known local silvatic strains, despite frequent invasion of the domestic setting by infected adult vectors. Multilocus linkage indices indicate predominantly clonal parasite propagation among all populations. However, excess homozygosity among silvatic strains and raised heterozygosity among domestic populations suggest that some level of genetic recombination cannot be ruled out. The epidemiological significance of these findings is discussed.
publishDate 2009
dc.date.none.fl_str_mv 2009-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29958
Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; et al.; Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection; Public Library of Science; Plos Pathogens; 5; 5; 1-5-2009; 1-9; e1000410
1553-7366
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29958
identifier_str_mv Llewellyn, Martin S.; Miles, Michael A.; Carrasco, Hector Jeronimo; Lewis, Michael D.; Yeo, Matthew; et al.; Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection; Public Library of Science; Plos Pathogens; 5; 5; 1-5-2009; 1-9; e1000410
1553-7366
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1000410
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000410
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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