Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts

Autores
Nally, Maria Cristina; Pesce, Virginia Mercedes; Toro, Maria Eugenia; Castellanos, Lucia Ines; Vazquez, Fabio
Año de publicación
2009
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Botrytis cinerea is a major pathogen of grape. At present, control in conventional agriculture is mainly achieved through chemical strategies, which can also have many disadvantages, such as the public‟s growing concern for the human health conditions and the environmental pollution. One promising alternative to pesticides is the biological control, numerous studies indicated that some yeast species are ideal biocontrol agents, as they are natural plant epiphytic colonizers, nonpathogenic to plants and human beings in most cases and can rapidly proliferate. It has been reported that biological control was only effective when high concentrations of antagonist yeasts were applied. Objective: The aim of the present work was to study the efficacy of different concentrations of antagonistic yeasts in reducing the development of B.cinerea. Materials and Methods: A- Microorganisms: The pathogen B.cinerea was isolated from infected grapes. All yeast antagonists (15 strains of Saccharomyces cerevisiae and 1 of Schizosaccharomyces pombe) were originally isolated from grape surfaces and fermenting musts. They were selected because of their ability to control B.cinerea on grapes, screening them in vitro and in vivo. B- Tests on fruit: Biocontrol effectiveness was assessed on Red Globe grapes. The fruits were artificially wounded and inoculated with yeasts (10 5, 106 and 107 UFC/ml) and conidial suspension of B.cinerea (104 conidia/ml). Each sample, constituted by 9 berries and reproduced with three replicates for each yeast isolate, was incubated for 5 days at 25°C in a plastic box under high relative humidity (100%). After storage, the incidence of disease was analyzed in percentage and these were arcsin- transformed to angular data prior to ANOVA. Results: There were significant negative relationships between concentration of the antagonists and disease incidence (R2: range on 0.75 to 0.99). The efficacy was higher when a concentration 107 CFU/ml of antagonist was used. When yeast cell suspensions of 8 strains of S.cerevisiae (BSc5, BSc49, BSc81, BSc92, BSc121, BSc140, BSc175 and BSc203) and S.pombe BSc167 reached a concentration of 107 CFU/ml, no infection by B.cinerea was found in fruits treated. Two strains of S.cerevisiae: BSc49 and BSc140 were able to inhibit mycelial growth of grey mould when a concentration of 10 6 CFU/ml of yeasts was inoculated. Conclusions: The concentrations of antagonist had significant effects on biocontrol effectiveness: the higher the concentration of yeast the better biocontrol activity of the antagonist had. When yeast was at 10 7 CFU/ml, the best control was obtained and this concentration was lower than those reported by other investigators.
Fil: Nally, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
VI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
Materia
Grey mould
Grapes
San Juan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195005
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195005
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeastsNally, Maria CristinaPesce, Virginia MercedesToro, Maria EugeniaCastellanos, Lucia InesVazquez, FabioGrey mouldGrapesSan Juanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Botrytis cinerea is a major pathogen of grape. At present, control in conventional agriculture is mainly achieved through chemical strategies, which can also have many disadvantages, such as the public‟s growing concern for the human health conditions and the environmental pollution. One promising alternative to pesticides is the biological control, numerous studies indicated that some yeast species are ideal biocontrol agents, as they are natural plant epiphytic colonizers, nonpathogenic to plants and human beings in most cases and can rapidly proliferate. It has been reported that biological control was only effective when high concentrations of antagonist yeasts were applied. Objective: The aim of the present work was to study the efficacy of different concentrations of antagonistic yeasts in reducing the development of B.cinerea. Materials and Methods: A- Microorganisms: The pathogen B.cinerea was isolated from infected grapes. All yeast antagonists (15 strains of Saccharomyces cerevisiae and 1 of Schizosaccharomyces pombe) were originally isolated from grape surfaces and fermenting musts. They were selected because of their ability to control B.cinerea on grapes, screening them in vitro and in vivo. B- Tests on fruit: Biocontrol effectiveness was assessed on Red Globe grapes. The fruits were artificially wounded and inoculated with yeasts (10 5, 106 and 107 UFC/ml) and conidial suspension of B.cinerea (104 conidia/ml). Each sample, constituted by 9 berries and reproduced with three replicates for each yeast isolate, was incubated for 5 days at 25°C in a plastic box under high relative humidity (100%). After storage, the incidence of disease was analyzed in percentage and these were arcsin- transformed to angular data prior to ANOVA. Results: There were significant negative relationships between concentration of the antagonists and disease incidence (R2: range on 0.75 to 0.99). The efficacy was higher when a concentration 107 CFU/ml of antagonist was used. When yeast cell suspensions of 8 strains of S.cerevisiae (BSc5, BSc49, BSc81, BSc92, BSc121, BSc140, BSc175 and BSc203) and S.pombe BSc167 reached a concentration of 107 CFU/ml, no infection by B.cinerea was found in fruits treated. Two strains of S.cerevisiae: BSc49 and BSc140 were able to inhibit mycelial growth of grey mould when a concentration of 10 6 CFU/ml of yeasts was inoculated. Conclusions: The concentrations of antagonist had significant effects on biocontrol effectiveness: the higher the concentration of yeast the better biocontrol activity of the antagonist had. When yeast was at 10 7 CFU/ml, the best control was obtained and this concentration was lower than those reported by other investigators.Fil: Nally, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaVI Congreso Argentino de Microbiología GeneralCórdobaArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195005Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts; VI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2009; 63-63CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2009.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:12Zoai:ri.conicet.gov.ar:11336/195005instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:13.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
title Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
spellingShingle Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
Nally, Maria Cristina
Grey mould
Grapes
San Juan
title_short Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
title_full Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
title_fullStr Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
title_full_unstemmed Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
title_sort Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts
dc.creator.none.fl_str_mv Nally, Maria Cristina
Pesce, Virginia Mercedes
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author Nally, Maria Cristina
author_facet Nally, Maria Cristina
Pesce, Virginia Mercedes
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author_role author
author2 Pesce, Virginia Mercedes
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Grey mould
Grapes
San Juan
topic Grey mould
Grapes
San Juan
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Botrytis cinerea is a major pathogen of grape. At present, control in conventional agriculture is mainly achieved through chemical strategies, which can also have many disadvantages, such as the public‟s growing concern for the human health conditions and the environmental pollution. One promising alternative to pesticides is the biological control, numerous studies indicated that some yeast species are ideal biocontrol agents, as they are natural plant epiphytic colonizers, nonpathogenic to plants and human beings in most cases and can rapidly proliferate. It has been reported that biological control was only effective when high concentrations of antagonist yeasts were applied. Objective: The aim of the present work was to study the efficacy of different concentrations of antagonistic yeasts in reducing the development of B.cinerea. Materials and Methods: A- Microorganisms: The pathogen B.cinerea was isolated from infected grapes. All yeast antagonists (15 strains of Saccharomyces cerevisiae and 1 of Schizosaccharomyces pombe) were originally isolated from grape surfaces and fermenting musts. They were selected because of their ability to control B.cinerea on grapes, screening them in vitro and in vivo. B- Tests on fruit: Biocontrol effectiveness was assessed on Red Globe grapes. The fruits were artificially wounded and inoculated with yeasts (10 5, 106 and 107 UFC/ml) and conidial suspension of B.cinerea (104 conidia/ml). Each sample, constituted by 9 berries and reproduced with three replicates for each yeast isolate, was incubated for 5 days at 25°C in a plastic box under high relative humidity (100%). After storage, the incidence of disease was analyzed in percentage and these were arcsin- transformed to angular data prior to ANOVA. Results: There were significant negative relationships between concentration of the antagonists and disease incidence (R2: range on 0.75 to 0.99). The efficacy was higher when a concentration 107 CFU/ml of antagonist was used. When yeast cell suspensions of 8 strains of S.cerevisiae (BSc5, BSc49, BSc81, BSc92, BSc121, BSc140, BSc175 and BSc203) and S.pombe BSc167 reached a concentration of 107 CFU/ml, no infection by B.cinerea was found in fruits treated. Two strains of S.cerevisiae: BSc49 and BSc140 were able to inhibit mycelial growth of grey mould when a concentration of 10 6 CFU/ml of yeasts was inoculated. Conclusions: The concentrations of antagonist had significant effects on biocontrol effectiveness: the higher the concentration of yeast the better biocontrol activity of the antagonist had. When yeast was at 10 7 CFU/ml, the best control was obtained and this concentration was lower than those reported by other investigators.
Fil: Nally, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Pesce, Virginia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
VI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
description Introduction: Botrytis cinerea is a major pathogen of grape. At present, control in conventional agriculture is mainly achieved through chemical strategies, which can also have many disadvantages, such as the public‟s growing concern for the human health conditions and the environmental pollution. One promising alternative to pesticides is the biological control, numerous studies indicated that some yeast species are ideal biocontrol agents, as they are natural plant epiphytic colonizers, nonpathogenic to plants and human beings in most cases and can rapidly proliferate. It has been reported that biological control was only effective when high concentrations of antagonist yeasts were applied. Objective: The aim of the present work was to study the efficacy of different concentrations of antagonistic yeasts in reducing the development of B.cinerea. Materials and Methods: A- Microorganisms: The pathogen B.cinerea was isolated from infected grapes. All yeast antagonists (15 strains of Saccharomyces cerevisiae and 1 of Schizosaccharomyces pombe) were originally isolated from grape surfaces and fermenting musts. They were selected because of their ability to control B.cinerea on grapes, screening them in vitro and in vivo. B- Tests on fruit: Biocontrol effectiveness was assessed on Red Globe grapes. The fruits were artificially wounded and inoculated with yeasts (10 5, 106 and 107 UFC/ml) and conidial suspension of B.cinerea (104 conidia/ml). Each sample, constituted by 9 berries and reproduced with three replicates for each yeast isolate, was incubated for 5 days at 25°C in a plastic box under high relative humidity (100%). After storage, the incidence of disease was analyzed in percentage and these were arcsin- transformed to angular data prior to ANOVA. Results: There were significant negative relationships between concentration of the antagonists and disease incidence (R2: range on 0.75 to 0.99). The efficacy was higher when a concentration 107 CFU/ml of antagonist was used. When yeast cell suspensions of 8 strains of S.cerevisiae (BSc5, BSc49, BSc81, BSc92, BSc121, BSc140, BSc175 and BSc203) and S.pombe BSc167 reached a concentration of 107 CFU/ml, no infection by B.cinerea was found in fruits treated. Two strains of S.cerevisiae: BSc49 and BSc140 were able to inhibit mycelial growth of grey mould when a concentration of 10 6 CFU/ml of yeasts was inoculated. Conclusions: The concentrations of antagonist had significant effects on biocontrol effectiveness: the higher the concentration of yeast the better biocontrol activity of the antagonist had. When yeast was at 10 7 CFU/ml, the best control was obtained and this concentration was lower than those reported by other investigators.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195005
Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts; VI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2009; 63-63
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195005
identifier_str_mv Determination of the incidence of grey mould on grapes of San Juan, Argentina after applying different concentrations of naturally occurring antagonist yeasts; VI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2009; 63-63
CONICET Digital
CONICET
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language spa
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dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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