Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots

Autores
Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Rodríguez Assaf, Leticia Anahí; Toro, Maria Eugenia; Castellanos, Lucia Ines; Vazquez, Fabio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to determine the putative modes of action of 59 viticultural yeasts (31 Saccharomyces and 28 non- Saccharomyces) that inhibited fungi isolated from sour and grey rot in grapes. Inhibition of fungal mycelial growth by metabolites, enzyme activities (laminarinases, chitinases), antifungal volatiles, competition for nutrients (siderophores, Niche Overlap Index (NOI)), inhibition of fungal spore germination and decreased germinal tube length and induction of resistance were assayed. Biofungicide yeasts were classified into "antifungal patterns", according to their mechanisms of action. Thirty isolates presented at least two of the mechanisms assayed. We propose that inhibition of fungal mycelial growth by metabolites, laminarinases, competition for nutrients, inhibition of fungal spore germination and decreased germinal tube length, and antifungal volatiles by Saccharomyces and non- Saccharomyces viticultural yeasts is used as putative biocontrol mechanisms against phytopathogenic fungi. Twenty-four different antifungal patterns were identified. Siderophore production (N)and a combination of siderophore production and NOI > 0.92 (M)were the most frequent antifungal patterns observed in the biofungicide yeasts assayed. Elucidation of these mechanisms could be useful for optimization of an inoculum formulation, resulting in a more consistent control of grey and sour rot with Saccharomyces and non- Saccharomyces biocontrol yeasts.
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Maturano, Yolanda Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rodríguez Assaf, Leticia Anahí. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Materia
ANTIFUNGAL PATTERNS
GRAPE
POSSIBLE ACTION MECHANISMS
SACCHAROMYCES BIOFUNGICIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147670
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rotsNally, Maria CristinaPesce, Virginia MercedesMaturano, Yolanda PaolaRodríguez Assaf, Leticia AnahíToro, Maria EugeniaCastellanos, Lucia InesVazquez, FabioANTIFUNGAL PATTERNSGRAPEPOSSIBLE ACTION MECHANISMSSACCHAROMYCES BIOFUNGICIDEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to determine the putative modes of action of 59 viticultural yeasts (31 Saccharomyces and 28 non- Saccharomyces) that inhibited fungi isolated from sour and grey rot in grapes. Inhibition of fungal mycelial growth by metabolites, enzyme activities (laminarinases, chitinases), antifungal volatiles, competition for nutrients (siderophores, Niche Overlap Index (NOI)), inhibition of fungal spore germination and decreased germinal tube length and induction of resistance were assayed. Biofungicide yeasts were classified into "antifungal patterns", according to their mechanisms of action. Thirty isolates presented at least two of the mechanisms assayed. We propose that inhibition of fungal mycelial growth by metabolites, laminarinases, competition for nutrients, inhibition of fungal spore germination and decreased germinal tube length, and antifungal volatiles by Saccharomyces and non- Saccharomyces viticultural yeasts is used as putative biocontrol mechanisms against phytopathogenic fungi. Twenty-four different antifungal patterns were identified. Siderophore production (N)and a combination of siderophore production and NOI > 0.92 (M)were the most frequent antifungal patterns observed in the biofungicide yeasts assayed. Elucidation of these mechanisms could be useful for optimization of an inoculum formulation, resulting in a more consistent control of grey and sour rot with Saccharomyces and non- Saccharomyces biocontrol yeasts.Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Maturano, Yolanda Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Rodríguez Assaf, Leticia Anahí. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaElsevier Science2015-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147670Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Rodríguez Assaf, Leticia Anahí; Toro, Maria Eugenia; et al.; Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots; Elsevier Science; International Journal of Food Microbiology; 204; 2-7-2015; 91-1000168-16051879-3460CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160515001634info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.ijfoodmicro.2015.03.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:37Zoai:ri.conicet.gov.ar:11336/147670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:37.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
title Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
spellingShingle Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
Nally, Maria Cristina
ANTIFUNGAL PATTERNS
GRAPE
POSSIBLE ACTION MECHANISMS
SACCHAROMYCES BIOFUNGICIDES
title_short Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
title_full Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
title_fullStr Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
title_full_unstemmed Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
title_sort Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots
dc.creator.none.fl_str_mv Nally, Maria Cristina
Pesce, Virginia Mercedes
Maturano, Yolanda Paola
Rodríguez Assaf, Leticia Anahí
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author Nally, Maria Cristina
author_facet Nally, Maria Cristina
Pesce, Virginia Mercedes
Maturano, Yolanda Paola
Rodríguez Assaf, Leticia Anahí
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author_role author
author2 Pesce, Virginia Mercedes
Maturano, Yolanda Paola
Rodríguez Assaf, Leticia Anahí
Toro, Maria Eugenia
Castellanos, Lucia Ines
Vazquez, Fabio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIFUNGAL PATTERNS
GRAPE
POSSIBLE ACTION MECHANISMS
SACCHAROMYCES BIOFUNGICIDES
topic ANTIFUNGAL PATTERNS
GRAPE
POSSIBLE ACTION MECHANISMS
SACCHAROMYCES BIOFUNGICIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to determine the putative modes of action of 59 viticultural yeasts (31 Saccharomyces and 28 non- Saccharomyces) that inhibited fungi isolated from sour and grey rot in grapes. Inhibition of fungal mycelial growth by metabolites, enzyme activities (laminarinases, chitinases), antifungal volatiles, competition for nutrients (siderophores, Niche Overlap Index (NOI)), inhibition of fungal spore germination and decreased germinal tube length and induction of resistance were assayed. Biofungicide yeasts were classified into "antifungal patterns", according to their mechanisms of action. Thirty isolates presented at least two of the mechanisms assayed. We propose that inhibition of fungal mycelial growth by metabolites, laminarinases, competition for nutrients, inhibition of fungal spore germination and decreased germinal tube length, and antifungal volatiles by Saccharomyces and non- Saccharomyces viticultural yeasts is used as putative biocontrol mechanisms against phytopathogenic fungi. Twenty-four different antifungal patterns were identified. Siderophore production (N)and a combination of siderophore production and NOI > 0.92 (M)were the most frequent antifungal patterns observed in the biofungicide yeasts assayed. Elucidation of these mechanisms could be useful for optimization of an inoculum formulation, resulting in a more consistent control of grey and sour rot with Saccharomyces and non- Saccharomyces biocontrol yeasts.
Fil: Nally, Maria Cristina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Pesce, Virginia Mercedes. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Maturano, Yolanda Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rodríguez Assaf, Leticia Anahí. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Toro, Maria Eugenia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
description The aim of this study was to determine the putative modes of action of 59 viticultural yeasts (31 Saccharomyces and 28 non- Saccharomyces) that inhibited fungi isolated from sour and grey rot in grapes. Inhibition of fungal mycelial growth by metabolites, enzyme activities (laminarinases, chitinases), antifungal volatiles, competition for nutrients (siderophores, Niche Overlap Index (NOI)), inhibition of fungal spore germination and decreased germinal tube length and induction of resistance were assayed. Biofungicide yeasts were classified into "antifungal patterns", according to their mechanisms of action. Thirty isolates presented at least two of the mechanisms assayed. We propose that inhibition of fungal mycelial growth by metabolites, laminarinases, competition for nutrients, inhibition of fungal spore germination and decreased germinal tube length, and antifungal volatiles by Saccharomyces and non- Saccharomyces viticultural yeasts is used as putative biocontrol mechanisms against phytopathogenic fungi. Twenty-four different antifungal patterns were identified. Siderophore production (N)and a combination of siderophore production and NOI > 0.92 (M)were the most frequent antifungal patterns observed in the biofungicide yeasts assayed. Elucidation of these mechanisms could be useful for optimization of an inoculum formulation, resulting in a more consistent control of grey and sour rot with Saccharomyces and non- Saccharomyces biocontrol yeasts.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147670
Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Rodríguez Assaf, Leticia Anahí; Toro, Maria Eugenia; et al.; Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots; Elsevier Science; International Journal of Food Microbiology; 204; 2-7-2015; 91-100
0168-1605
1879-3460
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147670
identifier_str_mv Nally, Maria Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Rodríguez Assaf, Leticia Anahí; Toro, Maria Eugenia; et al.; Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots; Elsevier Science; International Journal of Food Microbiology; 204; 2-7-2015; 91-100
0168-1605
1879-3460
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160515001634
info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.ijfoodmicro.2015.03.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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