Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Hetero...

Autores
Ramirez, Maria Soledad; Xie, Gang; Traglia, German Matias; Johnson, Shannon L.; Davenport, Karen W.; Van Duin, David; Ramazani, Azam; Perez, Federico; Jacobs, Michael R.; Sherratt, David J.; Bonomo, Robert A.; Chain, Patrick S. G.; Tolmasky, Marcelo E.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.
Fil: Ramirez, Maria Soledad. California State University Fullerton; Estados Unidos
Fil: Xie, Gang. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Johnson, Shannon L.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Davenport, Karen W.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Van Duin, David. University of North Carolina; Estados Unidos
Fil: Ramazani, Azam. California State University Fullerton; Estados Unidos. University of Oxford; Reino Unido
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos
Fil: Jacobs, Michael R.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Sherratt, David J.. University of Oxford; Reino Unido
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Chain, Patrick S. G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Tolmasky, Marcelo E.. California State University Fullerton; Estados Unidos
Materia
Whole Genome Mapping
Optical Map
St-258
Carbapenem
Kpc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47880
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47880
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity ZonesRamirez, Maria SoledadXie, GangTraglia, German MatiasJohnson, Shannon L.Davenport, Karen W.Van Duin, DavidRamazani, AzamPerez, FedericoJacobs, Michael R.Sherratt, David J.Bonomo, Robert A.Chain, Patrick S. G.Tolmasky, Marcelo E.Whole Genome MappingOptical MapSt-258CarbapenemKpchttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.Fil: Ramirez, Maria Soledad. California State University Fullerton; Estados UnidosFil: Xie, Gang. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Johnson, Shannon L.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Davenport, Karen W.. Los Alamos National High Magnetic Field Laboratory; Estados UnidosFil: Van Duin, David. University of North Carolina; Estados UnidosFil: Ramazani, Azam. California State University Fullerton; Estados Unidos. University of Oxford; Reino UnidoFil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados UnidosFil: Jacobs, Michael R.. Case Western Reserve University School of Medicine; Estados UnidosFil: Sherratt, David J.. University of Oxford; Reino UnidoFil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados UnidosFil: Chain, Patrick S. G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Tolmasky, Marcelo E.. California State University Fullerton; Estados UnidosOxford Journal2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47880Ramirez, Maria Soledad; Xie, Gang; Traglia, German Matias; Johnson, Shannon L.; Davenport, Karen W.; et al.; Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones; Oxford Journal; Genome Biology and Evolution; 8; 6; 6-2016; 2036-20431759-6653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evw135info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/8/6/2036/2574118info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:36Zoai:ri.conicet.gov.ar:11336/47880instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:37.012CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
title Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
spellingShingle Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
Ramirez, Maria Soledad
Whole Genome Mapping
Optical Map
St-258
Carbapenem
Kpc
title_short Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
title_full Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
title_fullStr Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
title_full_unstemmed Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
title_sort Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones
dc.creator.none.fl_str_mv Ramirez, Maria Soledad
Xie, Gang
Traglia, German Matias
Johnson, Shannon L.
Davenport, Karen W.
Van Duin, David
Ramazani, Azam
Perez, Federico
Jacobs, Michael R.
Sherratt, David J.
Bonomo, Robert A.
Chain, Patrick S. G.
Tolmasky, Marcelo E.
author Ramirez, Maria Soledad
author_facet Ramirez, Maria Soledad
Xie, Gang
Traglia, German Matias
Johnson, Shannon L.
Davenport, Karen W.
Van Duin, David
Ramazani, Azam
Perez, Federico
Jacobs, Michael R.
Sherratt, David J.
Bonomo, Robert A.
Chain, Patrick S. G.
Tolmasky, Marcelo E.
author_role author
author2 Xie, Gang
Traglia, German Matias
Johnson, Shannon L.
Davenport, Karen W.
Van Duin, David
Ramazani, Azam
Perez, Federico
Jacobs, Michael R.
Sherratt, David J.
Bonomo, Robert A.
Chain, Patrick S. G.
Tolmasky, Marcelo E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Whole Genome Mapping
Optical Map
St-258
Carbapenem
Kpc
topic Whole Genome Mapping
Optical Map
St-258
Carbapenem
Kpc
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.
Fil: Ramirez, Maria Soledad. California State University Fullerton; Estados Unidos
Fil: Xie, Gang. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Johnson, Shannon L.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Davenport, Karen W.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Van Duin, David. University of North Carolina; Estados Unidos
Fil: Ramazani, Azam. California State University Fullerton; Estados Unidos. University of Oxford; Reino Unido
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos
Fil: Jacobs, Michael R.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Sherratt, David J.. University of Oxford; Reino Unido
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Chain, Patrick S. G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Tolmasky, Marcelo E.. California State University Fullerton; Estados Unidos
description Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47880
Ramirez, Maria Soledad; Xie, Gang; Traglia, German Matias; Johnson, Shannon L.; Davenport, Karen W.; et al.; Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones; Oxford Journal; Genome Biology and Evolution; 8; 6; 6-2016; 2036-2043
1759-6653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47880
identifier_str_mv Ramirez, Maria Soledad; Xie, Gang; Traglia, German Matias; Johnson, Shannon L.; Davenport, Karen W.; et al.; Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones; Oxford Journal; Genome Biology and Evolution; 8; 6; 6-2016; 2036-2043
1759-6653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evw135
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/8/6/2036/2574118
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford Journal
publisher.none.fl_str_mv Oxford Journal
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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