Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)

Autores: Barros, Ana Luisa A. N.; Silva, Vladimir C.; Ribeiro Junior, Atvaldo F.; Cardozo, Miguel G.; Costa, Samuel R.; Moraes, Carolina B.; Barbosa, Cecilia G.; Coleone, Alex P.; Simões, Rafael P.; Cabral, Wanessa F.; Falcão, Raul M.; Vasconcelos, Andreanne G.; Rocha, Jefferson A.; Arcanjo, Daniel D. R.; Batagin Neto, Augusto; Borges, Tatiana K. S.; Gonçalves, João; Brand, Guilherme D.; Freitas Junior, Lucio H.G.; Eaton, Peter; Marani, Mariela Mirta; Kato, Massuo J.; Plácido, Alexandra; Leite, José Roberto S. A.
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are strategically relevant to the research and development of innovative drugs for viral and bacterial treatment and disease management. Amphibian species possess a diverse array of compounds, including antimicrobial peptides. This study identified the first bioactive peptide from Salamandra salamandra in a transcriptome analysis. The synthetic peptide sequence, which belongs to the defensin family, was characterized through MALDI TOF/TOF mass spectrometry. Molecular docking assays hypothesized the interaction between the identified peptide and the active binding site of the spike WT RBD/hACE2 complex. Although additional studies are required, the preliminary evaluation of the antiviral potential of synthetic SS-I was conducted through an in vitro cell-based SARS-CoV-2 infection assay. Additionally, the cytotoxic and hemolytic effects of the synthesized peptide were assessed. These preliminary findings highlighted the potential of SS-I as a chemical scaffold for drug development against COVID-19, hindering viral infection. The peptide demonstrated hemolytic activity while not exhibiting cytotoxicity at the antiviral concentration.
Fil: Barros, Ana Luisa A. N.. Universidade do Brasília; Brasil
Fil: Silva, Vladimir C.. Universidade do Brasília; Brasil
Fil: Ribeiro Junior, Atvaldo F.. Universidade do Brasília; Brasil
Fil: Cardozo, Miguel G.. Universidade do Brasília; Brasil. Universidade Nova de Lisboa; Portugal
Fil: Costa, Samuel R.. Universidade do Brasília; Brasil
Fil: Moraes, Carolina B.. Universidade de Sao Paulo; Brasil
Fil: Barbosa, Cecilia G.. Universidade de Sao Paulo; Brasil
Fil: Coleone, Alex P.. Universidade de Sao Paulo; Brasil
Fil: Simões, Rafael P.. Universidade de Sao Paulo; Brasil
Fil: Cabral, Wanessa F.. Universidade do Brasília; Brasil
Fil: Falcão, Raul M.. Universidade Federal do Rio Grande do Norte; Brasil
Fil: Vasconcelos, Andreanne G.. Universidade do Brasília; Brasil
Fil: Rocha, Jefferson A.. Universidade Federal Do Maranhao.; Brasil
Fil: Arcanjo, Daniel D. R.. Universidade Federal Do Piaui.; Brasil
Fil: Batagin Neto, Augusto. Universidade de Sao Paulo; Brasil
Fil: Borges, Tatiana K. S.. Universidade do Brasília; Brasil
Fil: Gonçalves, João. Universidade Nova de Lisboa; Portugal
Fil: Brand, Guilherme D.. Universidade do Brasília; Brasil
Fil: Freitas Junior, Lucio H.G.. Universidade de Sao Paulo; Brasil
Fil: Eaton, Peter. Universidad de Porto; Portugal
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Kato, Massuo J.. Universidade de Sao Paulo; Brasil
Fil: Plácido, Alexandra. Universidad de Porto; Portugal
Fil: Leite, José Roberto S. A.. Universidade do Brasília; Brasil
Materia: AMPHIBIANS
TRANSCRIPTOMICS
ANTIMICROBIAL PEPTIDES
BIOINFORMATICS
ANTIVIRAL ACTION
SARS-CoV-2 INFECTION
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/233645

Acceder

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network_name_str	CONICET Digital (CONICET)
spelling	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)Barros, Ana Luisa A. N.Silva, Vladimir C.Ribeiro Junior, Atvaldo F.Cardozo, Miguel G.Costa, Samuel R.Moraes, Carolina B.Barbosa, Cecilia G.Coleone, Alex P.Simões, Rafael P.Cabral, Wanessa F.Falcão, Raul M.Vasconcelos, Andreanne G.Rocha, Jefferson A.Arcanjo, Daniel D. R.Batagin Neto, AugustoBorges, Tatiana K. S.Gonçalves, JoãoBrand, Guilherme D.Freitas Junior, Lucio H.G.Eaton, PeterMarani, Mariela MirtaKato, Massuo J.Plácido, AlexandraLeite, José Roberto S. A.AMPHIBIANSTRANSCRIPTOMICSANTIMICROBIAL PEPTIDESBIOINFORMATICSANTIVIRAL ACTIONSARS-CoV-2 INFECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are strategically relevant to the research and development of innovative drugs for viral and bacterial treatment and disease management. Amphibian species possess a diverse array of compounds, including antimicrobial peptides. This study identified the first bioactive peptide from Salamandra salamandra in a transcriptome analysis. The synthetic peptide sequence, which belongs to the defensin family, was characterized through MALDI TOF/TOF mass spectrometry. Molecular docking assays hypothesized the interaction between the identified peptide and the active binding site of the spike WT RBD/hACE2 complex. Although additional studies are required, the preliminary evaluation of the antiviral potential of synthetic SS-I was conducted through an in vitro cell-based SARS-CoV-2 infection assay. Additionally, the cytotoxic and hemolytic effects of the synthesized peptide were assessed. These preliminary findings highlighted the potential of SS-I as a chemical scaffold for drug development against COVID-19, hindering viral infection. The peptide demonstrated hemolytic activity while not exhibiting cytotoxicity at the antiviral concentration.Fil: Barros, Ana Luisa A. N.. Universidade do Brasília; BrasilFil: Silva, Vladimir C.. Universidade do Brasília; BrasilFil: Ribeiro Junior, Atvaldo F.. Universidade do Brasília; BrasilFil: Cardozo, Miguel G.. Universidade do Brasília; Brasil. Universidade Nova de Lisboa; PortugalFil: Costa, Samuel R.. Universidade do Brasília; BrasilFil: Moraes, Carolina B.. Universidade de Sao Paulo; BrasilFil: Barbosa, Cecilia G.. Universidade de Sao Paulo; BrasilFil: Coleone, Alex P.. Universidade de Sao Paulo; BrasilFil: Simões, Rafael P.. Universidade de Sao Paulo; BrasilFil: Cabral, Wanessa F.. Universidade do Brasília; BrasilFil: Falcão, Raul M.. Universidade Federal do Rio Grande do Norte; BrasilFil: Vasconcelos, Andreanne G.. Universidade do Brasília; BrasilFil: Rocha, Jefferson A.. Universidade Federal Do Maranhao.; BrasilFil: Arcanjo, Daniel D. R.. Universidade Federal Do Piaui.; BrasilFil: Batagin Neto, Augusto. Universidade de Sao Paulo; BrasilFil: Borges, Tatiana K. S.. Universidade do Brasília; BrasilFil: Gonçalves, João. Universidade Nova de Lisboa; PortugalFil: Brand, Guilherme D.. Universidade do Brasília; BrasilFil: Freitas Junior, Lucio H.G.. Universidade de Sao Paulo; BrasilFil: Eaton, Peter. Universidad de Porto; PortugalFil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Kato, Massuo J.. Universidade de Sao Paulo; BrasilFil: Plácido, Alexandra. Universidad de Porto; PortugalFil: Leite, José Roberto S. A.. Universidade do Brasília; BrasilMultidisciplinary Digital Publishing Institute2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/233645Barros, Ana Luisa A. N.; Silva, Vladimir C.; Ribeiro Junior, Atvaldo F.; Cardozo, Miguel G.; Costa, Samuel R.; et al.; Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 16; 2; 1-2024; 1-191999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics16020190info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/16/2/190info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:09Zoai:ri.conicet.gov.ar:11336/233645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:09.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
title	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
spellingShingle	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra) Barros, Ana Luisa A. N. AMPHIBIANS TRANSCRIPTOMICS ANTIMICROBIAL PEPTIDES BIOINFORMATICS ANTIVIRAL ACTION SARS-CoV-2 INFECTION
title_short	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
title_full	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
title_fullStr	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
title_full_unstemmed	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
title_sort	Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)
dc.creator.none.fl_str_mv	Barros, Ana Luisa A. N. Silva, Vladimir C. Ribeiro Junior, Atvaldo F. Cardozo, Miguel G. Costa, Samuel R. Moraes, Carolina B. Barbosa, Cecilia G. Coleone, Alex P. Simões, Rafael P. Cabral, Wanessa F. Falcão, Raul M. Vasconcelos, Andreanne G. Rocha, Jefferson A. Arcanjo, Daniel D. R. Batagin Neto, Augusto Borges, Tatiana K. S. Gonçalves, João Brand, Guilherme D. Freitas Junior, Lucio H.G. Eaton, Peter Marani, Mariela Mirta Kato, Massuo J. Plácido, Alexandra Leite, José Roberto S. A.
author	Barros, Ana Luisa A. N.
author_facet	Barros, Ana Luisa A. N. Silva, Vladimir C. Ribeiro Junior, Atvaldo F. Cardozo, Miguel G. Costa, Samuel R. Moraes, Carolina B. Barbosa, Cecilia G. Coleone, Alex P. Simões, Rafael P. Cabral, Wanessa F. Falcão, Raul M. Vasconcelos, Andreanne G. Rocha, Jefferson A. Arcanjo, Daniel D. R. Batagin Neto, Augusto Borges, Tatiana K. S. Gonçalves, João Brand, Guilherme D. Freitas Junior, Lucio H.G. Eaton, Peter Marani, Mariela Mirta Kato, Massuo J. Plácido, Alexandra Leite, José Roberto S. A.
author_role	author
author2	Silva, Vladimir C. Ribeiro Junior, Atvaldo F. Cardozo, Miguel G. Costa, Samuel R. Moraes, Carolina B. Barbosa, Cecilia G. Coleone, Alex P. Simões, Rafael P. Cabral, Wanessa F. Falcão, Raul M. Vasconcelos, Andreanne G. Rocha, Jefferson A. Arcanjo, Daniel D. R. Batagin Neto, Augusto Borges, Tatiana K. S. Gonçalves, João Brand, Guilherme D. Freitas Junior, Lucio H.G. Eaton, Peter Marani, Mariela Mirta Kato, Massuo J. Plácido, Alexandra Leite, José Roberto S. A.
author2_role	author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	AMPHIBIANS TRANSCRIPTOMICS ANTIMICROBIAL PEPTIDES BIOINFORMATICS ANTIVIRAL ACTION SARS-CoV-2 INFECTION
topic	AMPHIBIANS TRANSCRIPTOMICS ANTIMICROBIAL PEPTIDES BIOINFORMATICS ANTIVIRAL ACTION SARS-CoV-2 INFECTION
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are strategically relevant to the research and development of innovative drugs for viral and bacterial treatment and disease management. Amphibian species possess a diverse array of compounds, including antimicrobial peptides. This study identified the first bioactive peptide from Salamandra salamandra in a transcriptome analysis. The synthetic peptide sequence, which belongs to the defensin family, was characterized through MALDI TOF/TOF mass spectrometry. Molecular docking assays hypothesized the interaction between the identified peptide and the active binding site of the spike WT RBD/hACE2 complex. Although additional studies are required, the preliminary evaluation of the antiviral potential of synthetic SS-I was conducted through an in vitro cell-based SARS-CoV-2 infection assay. Additionally, the cytotoxic and hemolytic effects of the synthesized peptide were assessed. These preliminary findings highlighted the potential of SS-I as a chemical scaffold for drug development against COVID-19, hindering viral infection. The peptide demonstrated hemolytic activity while not exhibiting cytotoxicity at the antiviral concentration. Fil: Barros, Ana Luisa A. N.. Universidade do Brasília; Brasil Fil: Silva, Vladimir C.. Universidade do Brasília; Brasil Fil: Ribeiro Junior, Atvaldo F.. Universidade do Brasília; Brasil Fil: Cardozo, Miguel G.. Universidade do Brasília; Brasil. Universidade Nova de Lisboa; Portugal Fil: Costa, Samuel R.. Universidade do Brasília; Brasil Fil: Moraes, Carolina B.. Universidade de Sao Paulo; Brasil Fil: Barbosa, Cecilia G.. Universidade de Sao Paulo; Brasil Fil: Coleone, Alex P.. Universidade de Sao Paulo; Brasil Fil: Simões, Rafael P.. Universidade de Sao Paulo; Brasil Fil: Cabral, Wanessa F.. Universidade do Brasília; Brasil Fil: Falcão, Raul M.. Universidade Federal do Rio Grande do Norte; Brasil Fil: Vasconcelos, Andreanne G.. Universidade do Brasília; Brasil Fil: Rocha, Jefferson A.. Universidade Federal Do Maranhao.; Brasil Fil: Arcanjo, Daniel D. R.. Universidade Federal Do Piaui.; Brasil Fil: Batagin Neto, Augusto. Universidade de Sao Paulo; Brasil Fil: Borges, Tatiana K. S.. Universidade do Brasília; Brasil Fil: Gonçalves, João. Universidade Nova de Lisboa; Portugal Fil: Brand, Guilherme D.. Universidade do Brasília; Brasil Fil: Freitas Junior, Lucio H.G.. Universidade de Sao Paulo; Brasil Fil: Eaton, Peter. Universidad de Porto; Portugal Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina Fil: Kato, Massuo J.. Universidade de Sao Paulo; Brasil Fil: Plácido, Alexandra. Universidad de Porto; Portugal Fil: Leite, José Roberto S. A.. Universidade do Brasília; Brasil
description	The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are strategically relevant to the research and development of innovative drugs for viral and bacterial treatment and disease management. Amphibian species possess a diverse array of compounds, including antimicrobial peptides. This study identified the first bioactive peptide from Salamandra salamandra in a transcriptome analysis. The synthetic peptide sequence, which belongs to the defensin family, was characterized through MALDI TOF/TOF mass spectrometry. Molecular docking assays hypothesized the interaction between the identified peptide and the active binding site of the spike WT RBD/hACE2 complex. Although additional studies are required, the preliminary evaluation of the antiviral potential of synthetic SS-I was conducted through an in vitro cell-based SARS-CoV-2 infection assay. Additionally, the cytotoxic and hemolytic effects of the synthesized peptide were assessed. These preliminary findings highlighted the potential of SS-I as a chemical scaffold for drug development against COVID-19, hindering viral infection. The peptide demonstrated hemolytic activity while not exhibiting cytotoxicity at the antiviral concentration.
publishDate	2024
dc.date.none.fl_str_mv	2024-01
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/233645 Barros, Ana Luisa A. N.; Silva, Vladimir C.; Ribeiro Junior, Atvaldo F.; Cardozo, Miguel G.; Costa, Samuel R.; et al.; Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 16; 2; 1-2024; 1-19 1999-4923 CONICET Digital CONICET
url	http://hdl.handle.net/11336/233645
identifier_str_mv	Barros, Ana Luisa A. N.; Silva, Vladimir C.; Ribeiro Junior, Atvaldo F.; Cardozo, Miguel G.; Costa, Samuel R.; et al.; Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 16; 2; 1-2024; 1-19 1999-4923 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics16020190 info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/16/2/190
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv	Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.069144

Antiviral action against SARS-CoV-2 of a synthetic peptide based on a Novel defensin present in the transcriptome of the fire salamander (Salamandra salamandra)

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