Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages

Autores
Pascal, Stefanía Belén; Lorenzo Lopez, Juan Ramiro; Lucchesi, Paula Maria Alejandra; Krüger, Alejandra
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Shiga toxin (Stx)-producing Escherichia coli strains are foodborne pathogens that cancause severe human diseases, such as haemorrhagic colitis and haemolytic-uraemic syndrome. Stxsare encoded by bacteriophages (Stx phages) which show remarkable variations in genomecomposition and harbour several genes of unknown function. Recently, a gene encoding a sialateO-acetylesterase (NanS-p) was identified in some relevant Stx2a phages and it was suggested that itcould provide advantages for bacterial growth in the gut. The aim of this study was to analyse thepresence and sequence of nanS-p genes in available Stx2a phage genomes. A total of 59 DNAsequences of Stx2a phages were extracted from the NCBI GenBank database with the BLASTprogram using the stx2a sequence from phage 933W as query sequence, either as complete phagegenomes (45) or from bacterial genomes by subsequent analysis with PHASTER web server (14).Comparative analysis revealed that nanS-p was located downsteam stx2a in all genomes. Twentydifferent amino acid sequences of NanS-p were identified. Specifically, catalytic esterase domainsshowed only 11 possible sequences, with differences mainly observed in nine amino acid positions.Sequences corresponding to the N-terminal domain (DUF1737) showed three possible sequences,two of them closely related, while C-terminal domain was highly variable giving place to fourgroups with structural differences. Since sialate O-acetylesterase activity has been determined fromparticular Stx2a phages, new studies are necessary to evaluate if the NanS-p subtypes identified inthe present study also differ in their biological activity.
Fil: Pascal, Stefanía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lorenzo Lopez, Juan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Krüger, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
1st International Electronic Conference on Microbiology
Suiza
MDPI
Materia
STX2A BACTERIOPHAGES
SILIATE O-ACETYLESTERASE
SUBTYPES
SIALIC ACIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153956
Acceder
id CONICETDig_8e935afbdb1ad2076b2a4f86397da0b2
oai_identifier_str oai:ri.conicet.gov.ar:11336/153956
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophagesPascal, Stefanía BelénLorenzo Lopez, Juan RamiroLucchesi, Paula Maria AlejandraKrüger, AlejandraSTX2A BACTERIOPHAGESSILIATE O-ACETYLESTERASESUBTYPESSIALIC ACIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Shiga toxin (Stx)-producing Escherichia coli strains are foodborne pathogens that cancause severe human diseases, such as haemorrhagic colitis and haemolytic-uraemic syndrome. Stxsare encoded by bacteriophages (Stx phages) which show remarkable variations in genomecomposition and harbour several genes of unknown function. Recently, a gene encoding a sialateO-acetylesterase (NanS-p) was identified in some relevant Stx2a phages and it was suggested that itcould provide advantages for bacterial growth in the gut. The aim of this study was to analyse thepresence and sequence of nanS-p genes in available Stx2a phage genomes. A total of 59 DNAsequences of Stx2a phages were extracted from the NCBI GenBank database with the BLASTprogram using the stx2a sequence from phage 933W as query sequence, either as complete phagegenomes (45) or from bacterial genomes by subsequent analysis with PHASTER web server (14).Comparative analysis revealed that nanS-p was located downsteam stx2a in all genomes. Twentydifferent amino acid sequences of NanS-p were identified. Specifically, catalytic esterase domainsshowed only 11 possible sequences, with differences mainly observed in nine amino acid positions.Sequences corresponding to the N-terminal domain (DUF1737) showed three possible sequences,two of them closely related, while C-terminal domain was highly variable giving place to fourgroups with structural differences. Since sialate O-acetylesterase activity has been determined fromparticular Stx2a phages, new studies are necessary to evaluate if the NanS-p subtypes identified inthe present study also differ in their biological activity.Fil: Pascal, Stefanía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lorenzo Lopez, Juan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Krüger, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina1st International Electronic Conference on MicrobiologySuizaMDPIMDPI2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153956Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages; 1st International Electronic Conference on Microbiology; Suiza; 2020; 1-52504-3900CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2504-3900/66/1/15info:eu-repo/semantics/altIdentifier/doi/10.3390/proceedings2020066015Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:52Zoai:ri.conicet.gov.ar:11336/153956instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:53.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
title Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
spellingShingle Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
Pascal, Stefanía Belén
STX2A BACTERIOPHAGES
SILIATE O-ACETYLESTERASE
SUBTYPES
SIALIC ACIDS
title_short Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
title_full Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
title_fullStr Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
title_full_unstemmed Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
title_sort Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages
dc.creator.none.fl_str_mv Pascal, Stefanía Belén
Lorenzo Lopez, Juan Ramiro
Lucchesi, Paula Maria Alejandra
Krüger, Alejandra
author Pascal, Stefanía Belén
author_facet Pascal, Stefanía Belén
Lorenzo Lopez, Juan Ramiro
Lucchesi, Paula Maria Alejandra
Krüger, Alejandra
author_role author
author2 Lorenzo Lopez, Juan Ramiro
Lucchesi, Paula Maria Alejandra
Krüger, Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv STX2A BACTERIOPHAGES
SILIATE O-ACETYLESTERASE
SUBTYPES
SIALIC ACIDS
topic STX2A BACTERIOPHAGES
SILIATE O-ACETYLESTERASE
SUBTYPES
SIALIC ACIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Shiga toxin (Stx)-producing Escherichia coli strains are foodborne pathogens that cancause severe human diseases, such as haemorrhagic colitis and haemolytic-uraemic syndrome. Stxsare encoded by bacteriophages (Stx phages) which show remarkable variations in genomecomposition and harbour several genes of unknown function. Recently, a gene encoding a sialateO-acetylesterase (NanS-p) was identified in some relevant Stx2a phages and it was suggested that itcould provide advantages for bacterial growth in the gut. The aim of this study was to analyse thepresence and sequence of nanS-p genes in available Stx2a phage genomes. A total of 59 DNAsequences of Stx2a phages were extracted from the NCBI GenBank database with the BLASTprogram using the stx2a sequence from phage 933W as query sequence, either as complete phagegenomes (45) or from bacterial genomes by subsequent analysis with PHASTER web server (14).Comparative analysis revealed that nanS-p was located downsteam stx2a in all genomes. Twentydifferent amino acid sequences of NanS-p were identified. Specifically, catalytic esterase domainsshowed only 11 possible sequences, with differences mainly observed in nine amino acid positions.Sequences corresponding to the N-terminal domain (DUF1737) showed three possible sequences,two of them closely related, while C-terminal domain was highly variable giving place to fourgroups with structural differences. Since sialate O-acetylesterase activity has been determined fromparticular Stx2a phages, new studies are necessary to evaluate if the NanS-p subtypes identified inthe present study also differ in their biological activity.
Fil: Pascal, Stefanía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lorenzo Lopez, Juan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Krüger, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
1st International Electronic Conference on Microbiology
Suiza
MDPI
description Shiga toxin (Stx)-producing Escherichia coli strains are foodborne pathogens that cancause severe human diseases, such as haemorrhagic colitis and haemolytic-uraemic syndrome. Stxsare encoded by bacteriophages (Stx phages) which show remarkable variations in genomecomposition and harbour several genes of unknown function. Recently, a gene encoding a sialateO-acetylesterase (NanS-p) was identified in some relevant Stx2a phages and it was suggested that itcould provide advantages for bacterial growth in the gut. The aim of this study was to analyse thepresence and sequence of nanS-p genes in available Stx2a phage genomes. A total of 59 DNAsequences of Stx2a phages were extracted from the NCBI GenBank database with the BLASTprogram using the stx2a sequence from phage 933W as query sequence, either as complete phagegenomes (45) or from bacterial genomes by subsequent analysis with PHASTER web server (14).Comparative analysis revealed that nanS-p was located downsteam stx2a in all genomes. Twentydifferent amino acid sequences of NanS-p were identified. Specifically, catalytic esterase domainsshowed only 11 possible sequences, with differences mainly observed in nine amino acid positions.Sequences corresponding to the N-terminal domain (DUF1737) showed three possible sequences,two of them closely related, while C-terminal domain was highly variable giving place to fourgroups with structural differences. Since sialate O-acetylesterase activity has been determined fromparticular Stx2a phages, new studies are necessary to evaluate if the NanS-p subtypes identified inthe present study also differ in their biological activity.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153956
Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages; 1st International Electronic Conference on Microbiology; Suiza; 2020; 1-5
2504-3900
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153956
identifier_str_mv Subtypes of NanS-p sialate O-acetylesterase encoded by Stx2a bacteriophages; 1st International Electronic Conference on Microbiology; Suiza; 2020; 1-5
2504-3900
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3390/proceedings2020066015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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