Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns

Autores
Lujea, Noelia Carolina; Chiarini, Franco Ezequiel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Within Solanaceae, Nolana L. f. and Sclerophylax Miers are two genera that have deserved special attention because of their rare anatomical characteristics. However, they are poorly known from the cytogenetic perspective. In order to discuss their chromosome features in an evolutionary context, classical staining, chromomycin A3/4′ -6-diamidino-2-phenylindole (CMA/ DAPI) fluorescent banding and in situ hybridisation (FISH) with probes for the 18-5.8-26S and the 5S rDNA loci were applied to root tips of germinating seeds. All the species presented the chromosome number 2n = 24. Karyotypes were highly symmetric, with most chromosomes being metacentric and with a maximum of three submetacentric pairs in N. divaricata. The CMA/DAPI banding technique, assayed for the first time in Sclerophylax, showed CMA+ /DAPI− bands associated with nucleolar organiser regions (NORs) in the first metacentric chromosome pair in each species. The FISH technique (applied to four species of Sclerophylax and, for the first time, in one species of Nolana) showed that the 18-5.8-26S loci coincide with CMA+ /DAPI− bands. Three Sclerophylax species presented two pairs of 5S signals, whereas S. adnatifolia showed three. The rDNA loci resulted as asyntenic in Sclerophylax, but were localised in the same chromosome in N. divaricata. Despite the morphological peculiarities of Nolana and Sclerophylax, the chromosome number and karyotype features are consistent with the position of the two genera within the ‘x = 12 clade’, while the number and position of rDNA loci established the chromosome rearrangements, suggesting different evolutionary pathways with respect to their closest relatives, Lycium and Jaborosa.
Fil: Lujea, Noelia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
Heterochromatin
Karyotype
Nolana
Rdna
Sclerophyllax
South America
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24302
Acceder
id CONICETDig_8e7e4d4b08ae667b402f662e237344e7
oai_identifier_str oai:ri.conicet.gov.ar:11336/24302
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patternsLujea, Noelia CarolinaChiarini, Franco EzequielHeterochromatinKaryotypeNolanaRdnaSclerophyllaxSouth Americahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Within Solanaceae, Nolana L. f. and Sclerophylax Miers are two genera that have deserved special attention because of their rare anatomical characteristics. However, they are poorly known from the cytogenetic perspective. In order to discuss their chromosome features in an evolutionary context, classical staining, chromomycin A3/4′ -6-diamidino-2-phenylindole (CMA/ DAPI) fluorescent banding and in situ hybridisation (FISH) with probes for the 18-5.8-26S and the 5S rDNA loci were applied to root tips of germinating seeds. All the species presented the chromosome number 2n = 24. Karyotypes were highly symmetric, with most chromosomes being metacentric and with a maximum of three submetacentric pairs in N. divaricata. The CMA/DAPI banding technique, assayed for the first time in Sclerophylax, showed CMA+ /DAPI− bands associated with nucleolar organiser regions (NORs) in the first metacentric chromosome pair in each species. The FISH technique (applied to four species of Sclerophylax and, for the first time, in one species of Nolana) showed that the 18-5.8-26S loci coincide with CMA+ /DAPI− bands. Three Sclerophylax species presented two pairs of 5S signals, whereas S. adnatifolia showed three. The rDNA loci resulted as asyntenic in Sclerophylax, but were localised in the same chromosome in N. divaricata. Despite the morphological peculiarities of Nolana and Sclerophylax, the chromosome number and karyotype features are consistent with the position of the two genera within the ‘x = 12 clade’, while the number and position of rDNA loci established the chromosome rearrangements, suggesting different evolutionary pathways with respect to their closest relatives, Lycium and Jaborosa.Fil: Lujea, Noelia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaTaylor & Francis2017-01-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24302Lujea, Noelia Carolina; Chiarini, Franco Ezequiel; Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns; Taylor & Francis; New Zealand Journal of Botany; 55; 2; 5-1-2017; 163-1770028-825X1175-8643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2016.1269812info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/0028825X.2016.1269812info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:56Zoai:ri.conicet.gov.ar:11336/24302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:56.829CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
title Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
spellingShingle Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
Lujea, Noelia Carolina
Heterochromatin
Karyotype
Nolana
Rdna
Sclerophyllax
South America
title_short Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
title_full Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
title_fullStr Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
title_full_unstemmed Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
title_sort Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns
dc.creator.none.fl_str_mv Lujea, Noelia Carolina
Chiarini, Franco Ezequiel
author Lujea, Noelia Carolina
author_facet Lujea, Noelia Carolina
Chiarini, Franco Ezequiel
author_role author
author2 Chiarini, Franco Ezequiel
author2_role author
dc.subject.none.fl_str_mv Heterochromatin
Karyotype
Nolana
Rdna
Sclerophyllax
South America
topic Heterochromatin
Karyotype
Nolana
Rdna
Sclerophyllax
South America
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Within Solanaceae, Nolana L. f. and Sclerophylax Miers are two genera that have deserved special attention because of their rare anatomical characteristics. However, they are poorly known from the cytogenetic perspective. In order to discuss their chromosome features in an evolutionary context, classical staining, chromomycin A3/4′ -6-diamidino-2-phenylindole (CMA/ DAPI) fluorescent banding and in situ hybridisation (FISH) with probes for the 18-5.8-26S and the 5S rDNA loci were applied to root tips of germinating seeds. All the species presented the chromosome number 2n = 24. Karyotypes were highly symmetric, with most chromosomes being metacentric and with a maximum of three submetacentric pairs in N. divaricata. The CMA/DAPI banding technique, assayed for the first time in Sclerophylax, showed CMA+ /DAPI− bands associated with nucleolar organiser regions (NORs) in the first metacentric chromosome pair in each species. The FISH technique (applied to four species of Sclerophylax and, for the first time, in one species of Nolana) showed that the 18-5.8-26S loci coincide with CMA+ /DAPI− bands. Three Sclerophylax species presented two pairs of 5S signals, whereas S. adnatifolia showed three. The rDNA loci resulted as asyntenic in Sclerophylax, but were localised in the same chromosome in N. divaricata. Despite the morphological peculiarities of Nolana and Sclerophylax, the chromosome number and karyotype features are consistent with the position of the two genera within the ‘x = 12 clade’, while the number and position of rDNA loci established the chromosome rearrangements, suggesting different evolutionary pathways with respect to their closest relatives, Lycium and Jaborosa.
Fil: Lujea, Noelia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description Within Solanaceae, Nolana L. f. and Sclerophylax Miers are two genera that have deserved special attention because of their rare anatomical characteristics. However, they are poorly known from the cytogenetic perspective. In order to discuss their chromosome features in an evolutionary context, classical staining, chromomycin A3/4′ -6-diamidino-2-phenylindole (CMA/ DAPI) fluorescent banding and in situ hybridisation (FISH) with probes for the 18-5.8-26S and the 5S rDNA loci were applied to root tips of germinating seeds. All the species presented the chromosome number 2n = 24. Karyotypes were highly symmetric, with most chromosomes being metacentric and with a maximum of three submetacentric pairs in N. divaricata. The CMA/DAPI banding technique, assayed for the first time in Sclerophylax, showed CMA+ /DAPI− bands associated with nucleolar organiser regions (NORs) in the first metacentric chromosome pair in each species. The FISH technique (applied to four species of Sclerophylax and, for the first time, in one species of Nolana) showed that the 18-5.8-26S loci coincide with CMA+ /DAPI− bands. Three Sclerophylax species presented two pairs of 5S signals, whereas S. adnatifolia showed three. The rDNA loci resulted as asyntenic in Sclerophylax, but were localised in the same chromosome in N. divaricata. Despite the morphological peculiarities of Nolana and Sclerophylax, the chromosome number and karyotype features are consistent with the position of the two genera within the ‘x = 12 clade’, while the number and position of rDNA loci established the chromosome rearrangements, suggesting different evolutionary pathways with respect to their closest relatives, Lycium and Jaborosa.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24302
Lujea, Noelia Carolina; Chiarini, Franco Ezequiel; Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns; Taylor & Francis; New Zealand Journal of Botany; 55; 2; 5-1-2017; 163-177
0028-825X
1175-8643
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24302
identifier_str_mv Lujea, Noelia Carolina; Chiarini, Franco Ezequiel; Differentiation of Nolana and Sclerophylax (Solanaceae) by means of heterochromatin and rDNA patterns; Taylor & Francis; New Zealand Journal of Botany; 55; 2; 5-1-2017; 163-177
0028-825X
1175-8643
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2016.1269812
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/0028825X.2016.1269812
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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