RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells

Autores
Shan, B.; Gerez, Juan Atilio; Haedo, Mariana Raquel; Fuertes, Mariana; Theodoropoulou, M.; Buchfelder, M.; Losa, M.; Stalla, G.; Arzt, Eduardo Simon; Renner, U.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1a (HIF-1a). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is also a major regulator of vascular endothelial growth factor-A (VEGF-A), which is critically involved in the complex process of tumour neovascularisation. In this study, the expression and role of RSUME in pituitary tumours was studied. We found that RSUME mRNA was up-regulated in pituitary adenomas and significantly correlated with HIF-1a mRNA levels. Hypoxia (1% O2) or treatment with hypoxia-mimicking CoCl2 enhanced RSUME and HIF-1a expression, induced translocation of HIF-1a to the nuclei and stimulated VEGF-A production both in pituitary tumour cell lines and primary human pituitary adenoma cell cultures. When RSUME expression was specifically down-regulated by siRNA, the CoCl2-induced increase VEGF-A secretion was strongly reduced which was shown to be a consequence of the RSUME knockdown-associated reduction of HIF-1a synthesis. Thus, RSUME plays an important role in initiating pituitary tumour neovascularisation through regulating HIF-1a levels and subsequent VEGF-A production and may therefore be critically involved in pituitary adenoma progression.
Fil: Shan, B.. Max-Planck-Institut für Psychiatrie; Alemania
Fil: Gerez, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Haedo, Mariana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Fuertes, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Theodoropoulou, M.. Max-Planck-Institut für Psychiatrie; Alemania
Fil: Buchfelder, M.. Universitat Erlangen-Nuremberg; Alemania
Fil: Losa, M.. Istituto San Raffaele; Italia
Fil: Stalla, G.. Max-Planck-Institut für Psychiatrie; Alemania
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Renner, U.. Max-Planck-Institut für Psychiatrie; Alemania
Materia
Rsume
Pituitary Tumour
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16897
Acceder
id CONICETDig_8dada1a72ad453279b88da95ba56e46f
oai_identifier_str oai:ri.conicet.gov.ar:11336/16897
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cellsShan, B.Gerez, Juan AtilioHaedo, Mariana RaquelFuertes, MarianaTheodoropoulou, M.Buchfelder, M.Losa, M.Stalla, G.Arzt, Eduardo SimonRenner, U.RsumePituitary Tumourhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1a (HIF-1a). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is also a major regulator of vascular endothelial growth factor-A (VEGF-A), which is critically involved in the complex process of tumour neovascularisation. In this study, the expression and role of RSUME in pituitary tumours was studied. We found that RSUME mRNA was up-regulated in pituitary adenomas and significantly correlated with HIF-1a mRNA levels. Hypoxia (1% O2) or treatment with hypoxia-mimicking CoCl2 enhanced RSUME and HIF-1a expression, induced translocation of HIF-1a to the nuclei and stimulated VEGF-A production both in pituitary tumour cell lines and primary human pituitary adenoma cell cultures. When RSUME expression was specifically down-regulated by siRNA, the CoCl2-induced increase VEGF-A secretion was strongly reduced which was shown to be a consequence of the RSUME knockdown-associated reduction of HIF-1a synthesis. Thus, RSUME plays an important role in initiating pituitary tumour neovascularisation through regulating HIF-1a levels and subsequent VEGF-A production and may therefore be critically involved in pituitary adenoma progression.Fil: Shan, B.. Max-Planck-Institut für Psychiatrie; AlemaniaFil: Gerez, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Haedo, Mariana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Fuertes, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Theodoropoulou, M.. Max-Planck-Institut für Psychiatrie; AlemaniaFil: Buchfelder, M.. Universitat Erlangen-Nuremberg; AlemaniaFil: Losa, M.. Istituto San Raffaele; ItaliaFil: Stalla, G.. Max-Planck-Institut für Psychiatrie; AlemaniaFil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Renner, U.. Max-Planck-Institut für Psychiatrie; AlemaniaBioscientifica2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16897Shan, B.; Gerez, Juan Atilio; Haedo, Mariana Raquel; Fuertes, Mariana; Theodoropoulou, M.; et al.; RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells; Bioscientifica; Endocrine - Related Cancer; 19; 2-2012; 13-271351-0088enginfo:eu-repo/semantics/altIdentifier/doi/10.1530/ERC-11-0211info:eu-repo/semantics/altIdentifier/url/http://erc.endocrinology-journals.org/content/19/1/13info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:42Zoai:ri.conicet.gov.ar:11336/16897instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:43.185CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
spellingShingle RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
Shan, B.
Rsume
Pituitary Tumour
title_short RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_full RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_fullStr RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_full_unstemmed RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_sort RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
dc.creator.none.fl_str_mv Shan, B.
Gerez, Juan Atilio
Haedo, Mariana Raquel
Fuertes, Mariana
Theodoropoulou, M.
Buchfelder, M.
Losa, M.
Stalla, G.
Arzt, Eduardo Simon
Renner, U.
author Shan, B.
author_facet Shan, B.
Gerez, Juan Atilio
Haedo, Mariana Raquel
Fuertes, Mariana
Theodoropoulou, M.
Buchfelder, M.
Losa, M.
Stalla, G.
Arzt, Eduardo Simon
Renner, U.
author_role author
author2 Gerez, Juan Atilio
Haedo, Mariana Raquel
Fuertes, Mariana
Theodoropoulou, M.
Buchfelder, M.
Losa, M.
Stalla, G.
Arzt, Eduardo Simon
Renner, U.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rsume
Pituitary Tumour
topic Rsume
Pituitary Tumour
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1a (HIF-1a). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is also a major regulator of vascular endothelial growth factor-A (VEGF-A), which is critically involved in the complex process of tumour neovascularisation. In this study, the expression and role of RSUME in pituitary tumours was studied. We found that RSUME mRNA was up-regulated in pituitary adenomas and significantly correlated with HIF-1a mRNA levels. Hypoxia (1% O2) or treatment with hypoxia-mimicking CoCl2 enhanced RSUME and HIF-1a expression, induced translocation of HIF-1a to the nuclei and stimulated VEGF-A production both in pituitary tumour cell lines and primary human pituitary adenoma cell cultures. When RSUME expression was specifically down-regulated by siRNA, the CoCl2-induced increase VEGF-A secretion was strongly reduced which was shown to be a consequence of the RSUME knockdown-associated reduction of HIF-1a synthesis. Thus, RSUME plays an important role in initiating pituitary tumour neovascularisation through regulating HIF-1a levels and subsequent VEGF-A production and may therefore be critically involved in pituitary adenoma progression.
Fil: Shan, B.. Max-Planck-Institut für Psychiatrie; Alemania
Fil: Gerez, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Haedo, Mariana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Fuertes, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Theodoropoulou, M.. Max-Planck-Institut für Psychiatrie; Alemania
Fil: Buchfelder, M.. Universitat Erlangen-Nuremberg; Alemania
Fil: Losa, M.. Istituto San Raffaele; Italia
Fil: Stalla, G.. Max-Planck-Institut für Psychiatrie; Alemania
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Investigación En Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Renner, U.. Max-Planck-Institut für Psychiatrie; Alemania
description The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1a (HIF-1a). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is also a major regulator of vascular endothelial growth factor-A (VEGF-A), which is critically involved in the complex process of tumour neovascularisation. In this study, the expression and role of RSUME in pituitary tumours was studied. We found that RSUME mRNA was up-regulated in pituitary adenomas and significantly correlated with HIF-1a mRNA levels. Hypoxia (1% O2) or treatment with hypoxia-mimicking CoCl2 enhanced RSUME and HIF-1a expression, induced translocation of HIF-1a to the nuclei and stimulated VEGF-A production both in pituitary tumour cell lines and primary human pituitary adenoma cell cultures. When RSUME expression was specifically down-regulated by siRNA, the CoCl2-induced increase VEGF-A secretion was strongly reduced which was shown to be a consequence of the RSUME knockdown-associated reduction of HIF-1a synthesis. Thus, RSUME plays an important role in initiating pituitary tumour neovascularisation through regulating HIF-1a levels and subsequent VEGF-A production and may therefore be critically involved in pituitary adenoma progression.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16897
Shan, B.; Gerez, Juan Atilio; Haedo, Mariana Raquel; Fuertes, Mariana; Theodoropoulou, M.; et al.; RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells; Bioscientifica; Endocrine - Related Cancer; 19; 2-2012; 13-27
1351-0088
url http://hdl.handle.net/11336/16897
identifier_str_mv Shan, B.; Gerez, Juan Atilio; Haedo, Mariana Raquel; Fuertes, Mariana; Theodoropoulou, M.; et al.; RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells; Bioscientifica; Endocrine - Related Cancer; 19; 2-2012; 13-27
1351-0088
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/ERC-11-0211
info:eu-repo/semantics/altIdentifier/url/http://erc.endocrinology-journals.org/content/19/1/13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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