BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut

Autores
Guimarães, Patricia M; Garsmeur, Olivier; Proite, Karina; Leal Bertioli, Soraya C.M.; Seijo, José Guillermo; Chaine, Christian; Bertioli, David J; D'Hont, Angelique
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background. Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipansis with genome types AA and BB respectively. Results. We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC) vector, one for each of the diploid ancestral species. The libraries (AA and BB) are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes), and resistance gene analogues. Conclusion. These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.
Fil: Guimarães, Patricia M. Biotechnology Unit; Brasil
Fil: Garsmeur, Olivier. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Fil: Proite, Karina. Universidade do Brasília; Brasil. Biotechnology Unit; Brasil
Fil: Leal Bertioli, Soraya C.M.. Biotechnology Unit; Brasil
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Chaine, Christian. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Fil: Bertioli, David J. Genomic Sciences And Biotechnology Program; Brasil
Fil: D'Hont, Angelique. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Materia
PEANUT
GENOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36570
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36570
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanutGuimarães, Patricia MGarsmeur, OlivierProite, KarinaLeal Bertioli, Soraya C.M.Seijo, José GuillermoChaine, ChristianBertioli, David JD'Hont, AngeliquePEANUTGENOMEShttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background. Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipansis with genome types AA and BB respectively. Results. We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC) vector, one for each of the diploid ancestral species. The libraries (AA and BB) are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes), and resistance gene analogues. Conclusion. These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.Fil: Guimarães, Patricia M. Biotechnology Unit; BrasilFil: Garsmeur, Olivier. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; FranciaFil: Proite, Karina. Universidade do Brasília; Brasil. Biotechnology Unit; BrasilFil: Leal Bertioli, Soraya C.M.. Biotechnology Unit; BrasilFil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Chaine, Christian. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; FranciaFil: Bertioli, David J. Genomic Sciences And Biotechnology Program; BrasilFil: D'Hont, Angelique. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; FranciaBioMed Central2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36570Guimarães, Patricia M; Garsmeur, Olivier; Proite, Karina; Leal Bertioli, Soraya C.M.; Seijo, José Guillermo; et al.; BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut; BioMed Central; BMC Plant Biology; 8; 12-20081471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-8-14info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:02Zoai:ri.conicet.gov.ar:11336/36570instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:03.222CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
title BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
spellingShingle BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
Guimarães, Patricia M
PEANUT
GENOMES
title_short BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
title_full BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
title_fullStr BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
title_full_unstemmed BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
title_sort BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut
dc.creator.none.fl_str_mv Guimarães, Patricia M
Garsmeur, Olivier
Proite, Karina
Leal Bertioli, Soraya C.M.
Seijo, José Guillermo
Chaine, Christian
Bertioli, David J
D'Hont, Angelique
author Guimarães, Patricia M
author_facet Guimarães, Patricia M
Garsmeur, Olivier
Proite, Karina
Leal Bertioli, Soraya C.M.
Seijo, José Guillermo
Chaine, Christian
Bertioli, David J
D'Hont, Angelique
author_role author
author2 Garsmeur, Olivier
Proite, Karina
Leal Bertioli, Soraya C.M.
Seijo, José Guillermo
Chaine, Christian
Bertioli, David J
D'Hont, Angelique
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PEANUT
GENOMES
topic PEANUT
GENOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background. Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipansis with genome types AA and BB respectively. Results. We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC) vector, one for each of the diploid ancestral species. The libraries (AA and BB) are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes), and resistance gene analogues. Conclusion. These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.
Fil: Guimarães, Patricia M. Biotechnology Unit; Brasil
Fil: Garsmeur, Olivier. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Fil: Proite, Karina. Universidade do Brasília; Brasil. Biotechnology Unit; Brasil
Fil: Leal Bertioli, Soraya C.M.. Biotechnology Unit; Brasil
Fil: Seijo, José Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Chaine, Christian. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
Fil: Bertioli, David J. Genomic Sciences And Biotechnology Program; Brasil
Fil: D'Hont, Angelique. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
description Background. Cultivated peanut, Arachis hypogaea is an allotetraploid of recent origin, with an AABB genome. In common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. Therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. In contrast to cultivated peanut, most wild Arachis species are diploid with high genetic diversity. The study of diploid Arachis genomes is therefore attractive, both to simplify the construction of genetic and physical maps, and for the isolation and characterization of wild alleles. The most probable wild ancestors of cultivated peanut are A. duranensis and A. ipansis with genome types AA and BB respectively. Results. We constructed and characterized two large-insert libraries in Bacterial Artificial Chromosome (BAC) vector, one for each of the diploid ancestral species. The libraries (AA and BB) are respectively c. 7.4 and c. 5.3 genome equivalents with low organelle contamination and average insert sizes of 110 and 100 kb. Both libraries were used for the isolation of clones containing genetically mapped legume anchor markers (single copy genes), and resistance gene analogues. Conclusion. These diploid BAC libraries are important tools for the isolation of wild alleles conferring resistances to biotic stresses, comparisons of orthologous regions of the AA and BB genomes with each other and with other legume species, and will facilitate the construction of a physical map.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36570
Guimarães, Patricia M; Garsmeur, Olivier; Proite, Karina; Leal Bertioli, Soraya C.M.; Seijo, José Guillermo; et al.; BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut; BioMed Central; BMC Plant Biology; 8; 12-2008
1471-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36570
identifier_str_mv Guimarães, Patricia M; Garsmeur, Olivier; Proite, Karina; Leal Bertioli, Soraya C.M.; Seijo, José Guillermo; et al.; BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut; BioMed Central; BMC Plant Biology; 8; 12-2008
1471-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-8-14
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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